Radiation Medicine and Protection,
Journal Year:
2023,
Volume and Issue:
4(4), P. 214 - 220
Published: Oct. 24, 2023
To
investigate
the
impact
of
SWI/SNF
complex
on
heterochromatin
DNA
damage
repair
after
exposure
to
X-ray
irradiation,
in
order
explore
underlying
mechanism.
NIH3T3
and
MRC5
cells
were
treated
with
50
nmol/L
siRNA
targeting
subunits
(BRM,
ARID1A,
BRG1
SNF5),
YAP/TAZ.
At
24
h
transfection,
irradiated
0.5
1
Gy
X-rays.
20,
60
240
min
post-irradiation,
γH2AX
assay
was
performed
evaluate
radiation
response
total
or
heterochromatin.
Comet
used
determine
role
YAP/TAZ
when
4
BRM/BRG1
their
relationship
repair.
In
NIH3T3,
ARID1A
BRG1)
knock-down
increased
at
post-irradiation
(P
<
0.05),
while
SNF5
decreased
20
0.05).
MRC5,
BRM
Inconsistently,
did
not
affect
it,
Moreover,
Meanwhile,
Tail
Moment
comet
combining
significantly
compared
single
The
exhibited
varying
effects
promoted
accumulation
through
This
study
provides
a
novel
direction
for
sheds
light
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Oct. 10, 2024
Lung
cancer
is
one
of
the
most
common
types
malignant
worldwide,
causing
a
serious
social
and
economic
burden.
It
classified
into
non-small
cell
lung
(NSCLC)
small
cancer,
with
NSCLC
accounting
for
80-85%
cases.
Eukaryotic
translation
initiation
factor
4
gamma
1
(EIF4G1)
highly
expressed
in
NSCLC,
playing
an
important
role
regulating
tumor
growth,
angiogenesis,
transformation,
phagocytosis.
Ubiquitin-specific
protease
10
(USP10)
functions
as
deubiquitinating
enzyme
to
regulate
substrate
protein
deubiquitination
reverse
ubiquitin
proteasome
degradation
pathway.
Our
previous
study
identified
interaction
between
EIF4G1
USP10;
however,
their
regulatory
mechanism
remains
unclear.
Herein,
we
found
that
USP10
positively
regulates
cells.
An
vivo
ubiquitination
assay
demonstrated
by
USP10,
which
reversed
proteasomal
EIF4G1,
thereby
increasing
its
stability.
Upregulation
promoted
proliferation,
migration,
invasion
The
current
not
only
reveals
novel
through
but
also
demonstrates
potential
therapeutic
target
treat
NSCLC.
Life Science Alliance,
Journal Year:
2024,
Volume and Issue:
7(12), P. e202402935 - e202402935
Published: Oct. 4, 2024
Mucosal
healing
is
associated
with
better
clinical
outcomes
in
patients
inflammatory
bowel
disease.
But
the
epithelial-specific
contribution
to
mucosal
vivo
poorly
understood.
We
evaluated
an
acute
dextran
sulfate
sodium
mouse
model
that
shows
alleviated
colitis
response
after
loss
of
Smad4.
find
enhanced
epithelial
wound
alleviates
fibrotic
response.
Dextran
caused
increased
mesenchymal
collagen
deposition—indicative
fibrosis—within
a
week
WT
but
not
Smad4
KO
colon.
The
correlated
decreased
proliferation
WT,
whereas
uninterrupted
and
expanded
zone
were
observed
colon
epithelium.
Furthermore,
showed
extracellular
matrix
alterations
promote
regeneration.
Our
data
suggest
epithelium
key
determinant
vivo,
implicating
as
strategy
against
fibrosis
disease
patients.
Frontiers in Cardiovascular Medicine,
Journal Year:
2024,
Volume and Issue:
11
Published: Oct. 10, 2024
Inflammation
is
an
important
driver
of
hypertension
with
numerous
components,
and
there
a
paucity
research
on
the
specific
inflammatory
factors
that
induce
hypertension;
therefore,
we
wanted
to
investigate
relationship
between
hypertension.
Biomedicines,
Journal Year:
2024,
Volume and Issue:
12(12), P. 2771 - 2771
Published: Dec. 5, 2024
Gut
health
is
crucial
in
many
ways,
such
as
improving
human
general
and
enhancing
production
agricultural
animals.
To
maximize
the
effect
of
a
healthy
gastrointestinal
tract
(GIT),
an
understanding
regulation
intestinal
functions
needed.
Proper
depend
on
activity,
composition,
behavior
epithelial
cells
(IECs).
There
are
various
types
IECs,
including
enterocytes,
Paneth
cells,
enteroendocrine
(EECs),
goblet
tuft
M
stem
(IESCs),
each
with
unique
3D
structures
IEC
distributions.
Although
communication
between
IECs
other
cell
types,
immune
neurons,
has
been
intensively
reviewed,
different
rarely
addressed.
The
present
paper
overviews
networks
among
that
influence
functions.
Intestinal
absorption
regulated
by
incretins
derived
from
EECs
induce
nutrient
transporter
activity
enterocytes.
EECs,
enterocytes
release
signals
to
activate
Notch
signaling,
which
modulates
IESC
homeostasis,
proliferation
differentiation.
immunity
can
be
altered
via
cytokines
IECs.
Finally,
tools
for
investigating
have
discussed,
novel
model
utilizing
enteroids
considered
powerful
tool
research.
Overall,
importance
communication,
especially
cover
most
functional
regulating
pathways,
overviewed
this
paper.
Such
compilation
will
helpful
developing
strategies
maintaining
gut
health.
Radiation Medicine and Protection,
Journal Year:
2023,
Volume and Issue:
4(4), P. 214 - 220
Published: Oct. 24, 2023
To
investigate
the
impact
of
SWI/SNF
complex
on
heterochromatin
DNA
damage
repair
after
exposure
to
X-ray
irradiation,
in
order
explore
underlying
mechanism.
NIH3T3
and
MRC5
cells
were
treated
with
50
nmol/L
siRNA
targeting
subunits
(BRM,
ARID1A,
BRG1
SNF5),
YAP/TAZ.
At
24
h
transfection,
irradiated
0.5
1
Gy
X-rays.
20,
60
240
min
post-irradiation,
γH2AX
assay
was
performed
evaluate
radiation
response
total
or
heterochromatin.
Comet
used
determine
role
YAP/TAZ
when
4
BRM/BRG1
their
relationship
repair.
In
NIH3T3,
ARID1A
BRG1)
knock-down
increased
at
post-irradiation
(P
<
0.05),
while
SNF5
decreased
20
0.05).
MRC5,
BRM
Inconsistently,
did
not
affect
it,
Moreover,
Meanwhile,
Tail
Moment
comet
combining
significantly
compared
single
The
exhibited
varying
effects
promoted
accumulation
through
This
study
provides
a
novel
direction
for
sheds
light
