Simulator of the Human Intestinal Microbial Ecosystem (SHIME®): Current Developments, Applications, and Future Prospects

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1639 - 1639

Published: Dec. 6, 2024

The human gastrointestinal microbiota plays a vital role in maintaining host health and preventing diseases, prompting the creation of simulators to replicate this intricate system. Simulator Human Intestinal Microbial Ecosystem (SHIME®), multicompartment dynamic simulator, has emerged as pivotal vitro model for studying interactions interferences within gut microbiota. continuous real-time monitoring hallmarks, along with programmatically flexible setup, bestow SHIME® ability mimic entire intestinal ecosystem high dynamics stability, allowing evaluation various treatments on bowel controlled environment. This review outlines recent developments systems, including M-SHIME®, Twin-SHIME®, Triple-SHIME®, Toddle models, highlighting their applications fields food nutritional science, drug development, research, traditional Chinese medicine. Additionally, prospect integrating other advanced technologies is also discussed. findings underscore versatility technology, demonstrating its significant contributions current research potential future innovation microbiome-related fields.

Language: Английский

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Blueberry-derived exosome like nanovesicles carry RNA cargo into HIEC-6 cells and down-regulate LPS-induced inflammatory gene expression: a proof-of-concept study

Archives of Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown, P. 110266 - 110266

Published: Dec. 1, 2024

Language: Английский

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Roles of breast cancer resistance protein and organic anion transporting polypeptide 2B1 in gastrointestinal toxicity induced by SN-38 under inflammatory conditions

Toxicology Letters, Journal Year: 2024, Volume and Issue: 394, P. 57 - 65

Published: Feb. 27, 2024

Language: Английский

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Cell-Based In Vitro Models: Emerging Technologies for Enhanced Drug Permeability Prediction

International Research Journal of Modernization in Engineering Technology and Science, Journal Year: 2024, Volume and Issue: unknown

Published: April 21, 2024

Early drug development gets a boost with cell-based in vitro models for permeation.These cost-effective tools track absorption rates and mechanisms, using immortalized epithelial cells that mimic various barriers (gut, lung, skin, etc.).This review explores key the intriguing challenge of bridging gap between real-world human absorption.Refining these holds to optimizing discovery bringing promising candidates fruition.

Language: Английский

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A mitochondria-targeted nitric oxide probe with large Stokes shift for real-time imaging and evaluation of inflammatory bowel disease in situ

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1332, P. 343372 - 343372

Published: Oct. 24, 2024

Language: Английский

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Navigating the neuroinflammatory network: insights from diverse cell models

In vitro models, Journal Year: 2024, Volume and Issue: 3(1), P. 1 - 4

Published: March 12, 2024

In this Highlights article, we present insights into the use of simple cell lines in neuroinflammation research, highlighting key findings from our recent investigations. Simple lines, including HEK, PC12, SHSY5Y, and N2a cells, provide valuable critical signaling pathways hidden facets neuroinflammatory landscape. Focusing on specific outcomes, impact interleukin-6 (IL-6) acid-sensing ion channels (ASIC1a), study sheds light processes. Results showcase potential diverse models identifying molecules offer a multidimensional perspective intricate network, paving way for therapeutic interventions.

Language: Английский

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Recent advances in in vitro models simulating the female genital tract toward more effective intravaginal therapeutic delivery

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: 21(7), P. 1007 - 1027

Published: July 2, 2024

Intravaginal drug delivery has emerged as a promising avenue for treating spectrum of systemic and local female genital tract (FGT) conditions, using biomaterials carriers or scaffolds targeted efficient administration. Much effort been made to understand the natural barriers this route improve system achieve an therapeutic response.

Language: Английский

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Mini-colons predict drug toxicity in vitro

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(8), P. 1095 - 1096

Published: Aug. 1, 2024

Language: Английский

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Modulation of Photosensitizing Responses in Cell Culture Environments by Different Medium Components

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10016 - 10016

Published: Sept. 17, 2024

Many cell culture experiments are performed under light to evaluate the photodynamic or photosensitizing efficacy of various agents. In this study, modulation responses and phototoxicity conditions by different medium components was investigated. The significant levels reactive oxygen species (ROS) generated from DMEM, RPMI 1640, MEM were observed irradiation fluorescent (FL) white blue LEDs, indicating that these media have their own properties; DMEM showed most potent property. Phenol red-free (Pf-D) exhibited a stronger property than normal 1.31 1.25 times FL respectively; phenol red riboflavin-free (PRbf-D) did not show any properties. inhibitory effect on transmission more pronounced in RPMI, interference green LED greatest at 57.8 27.4%, disappeared Pf-D. containing riboflavin induced strong HaCaT keratinocytes generating H2O2 irradiation, which quenched sodium pyruvate media. presence serum also reduced phototoxicity; decreased content dependently. erythrosine B protoporphyrin IX sensitively PRbf-D DMEM. present results indicate several components, including riboflavin, red, pyruvate, serum, could modulate system inducing activation interfering with ROS generation.

Language: Английский

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An Easy-to-Use Arrayed Brain–Heart Chip

Biosensors, Journal Year: 2024, Volume and Issue: 14(11), P. 517 - 517

Published: Oct. 22, 2024

Multi-organ chips are effective at emulating human tissue and organ functions replicating the interactions among tissues organs. An arrayed brain-heart chip was introduced whose configuration comprises open culture chambers closed biomimetic vascular channels distributed in a horizontal pattern, separated from each other by an endothelial barrier based on fibrin matrix. A 300 μm-high 13.2 mm-long surrounded organoid chamber, thereby satisfying material transport requirements. Numerical simulations were used to analyze construction process of barriers order optimize structural design experimental manipulation, which exhibited high degree correlation with experiment results. In interconnective unit, cerebral organoid, cardiac cells co-cultured stably for minimum one week. The permeability recirculating perfusion enabled cross talk between organoids organoids, as well cells. This corroborated presence troponin I (cTnI) chamber observation invading matrix after week co-culture. simple manipulate, clearly visible under microscope, compatible automated pipetting devices, therefore had significant potential application.

Language: Английский

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