Making in vitro conditions more reflective of in vivo conditions for research on the teleost gastrointestinal tract DOI Creative Commons
Carol Bucking, Nicolas R. Bury, Henrik Sundh

et al.

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(19)

Published: Oct. 1, 2024

ABSTRACT To date, the majority of in vitro or ex vivo fish gastrointestinal research has been conducted under unrealistic conditions. In a living fish, ionic conditions, as well levels ammonia, pH, HCO3− and PCO2 differ considerably between different regions tract. These factors also from those saline often used gut research. Furthermore, oxygen gradient serosa to lumen is rarely considered: contrast serosa, hypoxic/anoxic environment. addition, microbiome plays significant role physiology, increasing complexity gut, but replicating microbial community for studies exceptionally difficult. However, there are ways which we can begin overcome these challenges. Firstly, luminal chemistry PO2 each compartment must be carefully considered. Secondly, although microbiological culture techniques improving, learn how maintain diversity seen vivo. Finally, studies, developing mucosal (luminal) solutions that more closely mimic conditions will better replicate physiological processes. Within field mammalian great advances ‘gut-on-chip’ devices providing tools conditions; adopting adapting this technology may assist initiatives. This Commentary aims make physiologists aware various issues identifies areas require further improvement.

Language: Английский

In Vitro Models for Investigating Intestinal Host–Pathogen Interactions DOI Creative Commons
Reece McCoy, Sophie Oldroyd,

Woojin Yang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 11(8)

Published: Dec. 28, 2023

Infectious diseases are increasingly recognized as a major threat worldwide due to the rise of antimicrobial resistance and emergence novel pathogens. In vitro models that can adequately mimic in vivo gastrointestinal physiology high demand elucidate mechanisms behind pathogen infectivity, aid design effective preventive therapeutic interventions. There exists trade-off between simple throughput those more complex physiologically relevant. The complexity model used shall be guided by biological question addressed. This review provides an overview structure function intestine developed emulate this. Conventional discussed addition emerging which employ engineering principles equip them with necessary advanced monitoring capabilities for intestinal host-pathogen interrogation. Limitations current future perspectives on field presented.

Language: Английский

Citations

12
Organoid models: applications and research advances in colorectal cancer DOI Creative Commons

Yijie Wu,

Sha Yu,

Xingpo Guo

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 7, 2025

This review summarizes the applications and research progress of organoid models in colorectal cancer research. First, high incidence mortality rates are introduced, emphasizing importance organoids as a model. Second, this provides detailed introduction to concept, biological properties, organoids, including their strengths mimicking structural functional aspects organs. article further analyzes adult stem cell-derived pluripotent discusses advancements for basic research, drug development, personalized treatment evaluation prediction, regenerative medicine. Finally, prospects applying technology its significant value improving patient survival rates. In conclusion, systematically explains highlighting tremendous potential promising

Language: Английский

Citations

0
Polarized Intestinal Cell Membrane‐on‐Chip for Bacterial Toxin Interaction Studies DOI Creative Commons
Reece McCoy,

Jeremy Treiber,

George G. Malliaras

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

The virulence of a pathogen is tied to the successful interaction between pathogen, or its toxins, and host cell. Polarized epithelial cells, constituting highly specialized cell monolayers, possess apical basolateral membrane regions with distinct functions structural compositions. Preserving these intricacies in membrane‐on‐a‐chip platforms important for retaining physiological relevance investigating host–pathogen interactions. Consequently, method obtaining populations vesicles representing membranes presented here, addition formation their respective supported lipid bilayers (SLBs) on PEDOT:PSS conducting polymer electrodes. localization A metalloprotease disintegrin (ADAM10) receptor Caco‐2 cells shown correlate increased response Staphylococcus aureus alpha hemolysin toxin compared model where ADAM10 absent. SLBs hemolysin‐containing extracellular (EVs) secreted by S. confirm direct effect toxin‐containing EVs reducing resistance plasma membrane. This technique could find use quantifying relative toxicity membrane, screening cognate receptors inhibitors, probing mechanism action.

Language: Английский

Citations

0
Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models DOI Creative Commons

Woojin Yang,

Eva Blahusova,

Reece McCoy

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

2D characterization of organoids by light microscopy with live cell imaging systems provides a powerful, rapid approach toward characterizing organoid growth patterns and behavior under different conditions high temporal resolution. However, current conventional analysis methods display critical flaws in their approximations, including inaccurate assumptions linear absorption kinetics inappropriate normalization darkness. Organoid darkness represents cellular shedding debris accumulation the lumen is thus proportional to surface area, rather than projection as conventionally used. This novel model image processing pipeline accounts for these shortcomings incorporating logarithmic parameters, noncumulative measure darkness, area‐normalized values which yield accurate highly reproducible representation kinetics.

Language: Английский

Citations

0
Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights DOI
Weifeng Hu, Yushen Wang,

Junlei Han

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review discusses advancements in organ-on-a-chip models, emphasizing gut, liver, and integrated gut–liver systems. It covers their applications disease modeling, toxicity testing, drug screening, as well future prospects.

Language: Английский

Citations

0
Dynamics of Spatial Organization of Bacterial Communities in a Tunable Flow Gut Microbiome‐on‐a‐Chip DOI Creative Commons
Jeeyeon Lee, Nishanth Venugopal Menon,

Hung Dong Truong

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Abstract The human intestine, a biomechanically active organ, generates cyclic mechanical forces crucial for maintaining its health and functions. Yet, the physiological impact of these on gut microbiota dynamics remains largely unexplored. In this study, we investigate how intestinal motility influences microbial communities within 3D gut‐like structure (µGut). To enable tunable flow Gut Microbiome‐on‐a‐Chip (tfGMoC) is developed that recapitulates expansion compression while allowing high‐magnification imaging stratified, biomimetic epithelium. Using deep learning‐based analysis, it found hydrodynamic organize by promoting distinct spatial exploration behaviors in microorganisms with varying characteristics. Empirical evidence demonstrates balanced composition, enhancing both diversity stability community ‐ key factors healthy microbiome. This leveraging new tfGMoC platform, uncovers previously unknown effects modulating organizations. will be critical deeper understanding host‐microbiome interactions emerging field microbiome therapeutics.

Language: Английский

Citations

0
Application of new approach methodologies for nonclinical safety assessment of drug candidates DOI
Mario Beilmann,

Karissa Adkins,

Harrie C. M. Boonen

et al.

Nature Reviews Drug Discovery, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Language: Английский

Citations

0
Engineered Tissue Models to Decode Host–Microbiota Interactions DOI Creative Commons
Miryam Adelfio, G. E. Callen, Xuesong He

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Abstract A mutualistic co‐evolution exists between the host and its associated microbiota in human body. Bacteria establish ecological niches various tissues of body, locally influencing their physiology functions, but also contributing to well‐being whole organism through systemic communication with other distant (axis). Emerging evidence indicates that when composition inhabiting niche changes toward a pathogenic state (dysbiosis) interactions become unbalanced, diseases may present. In addition, imbalances within single can cause dysbiosis organs. Current research efforts are focused on elucidating mechanisms leading dysbiosis, goal restoring tissue homeostasis. vitro models provide critical experimental platforms address this need, by reproducing cyto‐architecture high fidelity. This review surveys current host–microbiota strategies provides roadmap guide field further developing physiologically relevant niches, thus enabling investigation role health diseases. Lastly, given Food Drug Administration Modernization Act 2.0, highlights emerging support development validation new therapies market.

Language: Английский

Citations

0
In Situ Functionalization of Polar Polythiophene-Based Organic Electrochemical Transistor to Interface In Vitro Models DOI Creative Commons
Sebastian Buchmann,

P. Stoop,

Kim Roekevisch

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Organic mixed ionic-electronic conductors are promising materials for interfacing and monitoring biological systems, with the aim of overcoming current challenges based on mismatch between convectional inorganic conductors. The conjugated polymer/polyelectrolyte complex poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT/PSS) is, up to date, most widely used polymer in vitro or vivo measurements field organic bioelectronics. However, PEDOT/PSS electrochemical transistors (OECTs) limited by depletion mode operation lack chemical groups that enable synthetic modifications biointerfacing. Recently introduced thiophene-based polymers oligoether side chains can operate accumulation mode, their structure be tuned during synthesis, example, introduction hydroxylated chains. Here, we introduce a new polymer, p(g

Language: Английский

Citations

3
Mucus-on-a-chip: investigating the barrier properties of mucus with organic bioelectronics DOI Creative Commons
Reece McCoy,

Kaixin Wang,

Jeremy Treiber

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

A bioelectronic mucus-on-a-chip model is developed to monitor intestinal mucus barrier properties. The effects of a mucolytic agent and biofilm formation are investigated, offering insights into gastrointestinal research.

Language: Английский

Citations

3