Tryptophan As a New Member of RNA‐Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis

Advanced Science, Journal Year: 2024, Volume and Issue: 11(31)

Published: June 20, 2024

Abstract Essential amino acids (EAA) and microRNAs (miRs) control biological activity of a cell. Whether EAA regulates the miR has never been demonstrated. Here, as proof‐of‐concept, tryptophan (Trp, an EAA) complex containing Argonaute 2 (Ago2) miRs including miR‐193a (Trp/Ago2/miR‐193a) is identified. Trp binds miR‐193a‐3p interacts with Ago2. Trp/Ago2/miR‐193a increases via enhancing RNase activity. Other miR‐103 miR‐107 in enhance by targeting same genes. Mechanistically, Trp‐binding pockets PIWI domain Ago2 to mediated This newly formed Ago2/Trp/miR‐193a‐3p more efficient than alone inhibiting expression targeted genes colon cancer liver metastasis. The findings show that through communication RNA‐induced silencing complexes (RISC), which provides basis for based therapy.

Language: Английский

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The potential mechanism of MicroRNA involvement in the regulation of muscle development in weaned piglets by tryptophan and its metabolites

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 1, 2025

Muscle development is a key factor influencing the growth performance of piglets. Optimizing this developmental process crucial for enhancing breeding efficiency and economic profitability. Tryptophan (Trp) considered one limiting amino acids weaned piglets, plays an essential role in regulating feed intake, growth, muscle development. However, regulatory mechanisms by which Trp its derivatives influence piglets remain unclear. The aim study was to investigate pathways potential metabolites on In study, 10 healthy castrated male 28 days old weaned, were selected randomly assigned control group (CON, 0.14% Trp) high tryptophan (HT, 0.35% Trp), with 5 each group. After 7-day pre-feeding period, formal feeding began, after days, pigs slaughtered longissimus dorsi muscles collected transcriptome sequencing. results indicated that different dietary levels led identification sixteen differentially expressed microRNAs (DE miRNAs) Target gene functional enrichment analysis showed these DE miRNAs are involved cell proliferation, differentiation, protein deposition, through multiple biological pathways. Furthermore, we constructed protein-protein interaction (PPI) network target genes, enriched core cluster functions associated cellular signaling pathways, hormone release, Finally, qRT-PCR validated reliability accuracy RNA-seq results, revealing correlation coefficient 0.97 between two methods. This uncovers participate regulation mediated metabolites, providing theoretical basis practical guidance optimizing piglet management health improvement.

Language: Английский

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Integrated single cell and bulk RNA sequencing analyses reveal the impact of tryptophan metabolism on prognosis and immunotherapy in colon cancer

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 11, 2025

Language: Английский

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Comprehension of PTEN-Regulated MicroRNA Profiling in Oral Premalignant Lesions: A Critical Link to Early Detection of Oral Squamous Cell Carcinoma

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский

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