An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non‐Excitable Cells

Advanced Science, Journal Year: 2024, Volume and Issue: 11(20)

Published: March 25, 2024

Abstract Most animal cell types are classified as non‐excitable because they do not generate action potentials observed in excitable cells, such neurons and muscle cells. Thus, resolving voltage signals cells demands sensors with exceptionally high sensitivity. In this study, the ultrabright, ultrasensitive, calibratable genetically encoded sensor rEstus is developed using structure‐guided engineering. most sensitive resting range of offers a 3.6‐fold improvement brightness change for fast spikes over its precursor ASAP3. Using rEstus, it uncovered that membrane several lines (A375, HEK293T, MCF7) undergoes spontaneous endogenous alterations on second to millisecond timescale. Correlation analysis these optically recorded provides direct, real‐time readout electrical cell–cell coupling, showing visually connected A375 HEK293T also largely electrically connected, while MCF7 only weakly coupled. The presented work enhanced tools methods non‐invasive imaging living demonstrates limited but occur variety types.

Language: Английский

Oscillatory phenomena in electrophysiological networks: The coupling between cell bioelectricity and transcription

Computers in Biology and Medicine, Journal Year: 2024, Volume and Issue: 180, P. 108964 - 108964

Published: Aug. 5, 2024

Morphogenetic regulation during embryogenesis and regeneration rely on information transfer coordination between different regions. Here, we explore theoretically the coupling bioelectrical transcriptional oscillations at individual cell multicellular levels. The simulations, based a set of ion channels intercellular gap junctions, show that waves can electrophysiologically couple distant regions model network in phase antiphase oscillatory states include synchronization phenomena. In this way, regionalizations be encoded by potentials oscillate depolarized polarized states, thus allowing spatio-temporal coding. Because electric potential patterns characteristic development are correlated with spatial distributions signaling ions molecules, bioelectricity act as template for slow biochemical signals following hierarchy experimental times. particular, gradients to transcription rates give each single rough idea its location ensemble, controlling local differentiation processes switch off crucial parts genome.

Language: Английский

Multicellular adaptation to electrophysiological perturbations analyzed by deterministic and stochastic bioelectrical models

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 11, 2024

Cells can compensate a disruptive change in one ion channel by compensatory changes other channels. We have simulated the adaptation of multicellular aggregate non-excitable cells to electrophysiological perturbation produced external blocking cation channel. In biophysical model employed, we consider that this provokes cell depolarization opens voltage-gated calcium channel, thus allowing toxic Ca

Language: Английский