Bacteriophages as Targeted Therapeutic Vehicles: Challenges and Opportunities DOI Creative Commons

Srividhya Venkataraman,

Mehdi Shahgolzari,

Afagh Yavari

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(5), P. 469 - 469

Published: April 29, 2025

Bacteriophages, with their distinctive ability to selectively target host bacteria, stand out as a compelling tool in the realm of drug and gene delivery. Their assembly from proteins nucleic acids, coupled modifiable biologically unique properties, enables them serve efficient safe delivery systems. Unlike conventional nanocarriers, which face limitations such non-specific targeting, cytotoxicity, reduced transfection efficiency vivo, engineered phages exhibit promising potential overcome these hurdles improve outcomes. This review highlights bacteriophage-based systems innovative for delivering therapeutic agents. It explores strategies engineering bacteriophage, categorizes principal types employed delivery, evaluates applications disease therapy. provides intriguing details use natural therapy diseases cancer, bacterial viral infections, veterinary diseases, neurological disorders, well phage display technology generating monoclonal antibodies against various human diseases. Additionally, CRISPR-Cas9 genetically is elucidated. Furthermore, it critical analysis challenges associated phage-based systems, offering insights overcoming obstacles. By showcasing advancements integration into nanotechnology, this study underscores revolutionize approaches inspire future innovations medicine.

Language: Английский

Advances in Engineered Phages for Disease Treatment DOI Open Access
Xiaolin Hou,

Zhai Lin,

Laiying Fu

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

Abstract Phage therapy presents a promising solution for combating multidrug‐resistant (MDR) bacterial infections and other bacteria‐related diseases, attributed to their innate ability target lyse bacteria. Recent clinical successes, particularly in treating MDR‐related respiratory post‐surgical infections, validated the therapeutic potential of phage therapy. However, complex microenvironment within human body poses significant challenges activity efficacy vivo. To overcome these barriers, recent advances engineering have aimed enhance targeting accuracy, improve stability survivability, explore synergistic combinations with modalities. This review provides comprehensive overview therapy, emphasizing application engineered phages antibacterial tumor vaccine development. Furthermore, highlights current future trends advancing toward broader applications.

Language: Английский

Citations

1
Engineering Phage Nanocarriers Integrated with Bio‐Intelligent Plasmids for Personalized and Tunable Enzyme Delivery to Enhance Chemodynamic Therapy DOI Creative Commons
Xiaolin Hou, Bin Zhang, Kai Cheng

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(24)

Published: April 6, 2024

Abstract Customizable and number‐tunable enzyme delivery nanocarriers will be useful in tumor therapy. Herein, a phage vehicle, T4‐Lox‐DNA‐Fe (TLDF), which adeptly modulates numbers using display technology to remodel the microenvironment (TME) is presented. Regarding demand for lactic acid tumors, each engineered 720 lactate oxidase (Lox), contributing depletion of restructure tumor's energy metabolism. The vehicle incorporated dextran iron (Fe) with Fenton reaction capabilities. H 2 O generated through Lox catalytic reaction, amplifying supply iron‐based chemodynamic therapy (CDT). Drawing inspiration from erythropoietin (EPO) biosynthetic process, an EPO enhancer constructed impart EPO‐Keap1 plasmid (DNA) hypoxia‐activated functionality, disrupting redox homeostasis TME. consumes local oxygen, positive feedback between promotes expression kelch ECH Associated Protein 1 (Keap1). Consequently, downregulation antioxidant transcription factor Nrf2, synergy CDT, amplifies oxidative killing effect, leading suppression up 78%. This study seamlessly integrates adaptable T4 vehicles bio‐intelligent plasmids, presenting promising approach

Language: Английский

Citations

4
NIR-II photo- and sono-responsive hyaluronic acid-capped nanozymes for glioblastoma-targeting theranostics DOI

Huanran Qu,

Yuanyuan Zhang, Minghao Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141737 - 141737

Published: March 4, 2025

Language: Английский

Citations

0
Advances in nanotechnology for targeting cancer-associated fibroblasts: A review of multi-strategy drug delivery and preclinical insights DOI Creative Commons

Zhongsong Zhang,

Yujie Tang,

Dan Luo

et al.

APL Bioengineering, Journal Year: 2025, Volume and Issue: 9(1)

Published: March 1, 2025

Cancer-associated fibroblasts (CAFs) play a crucial role in the tumor microenvironment by promoting growth, immune evasion, and metastasis. Recently, drug delivery systems targeting CAFs have emerged as promising long-term effective approach to cancer treatment. Advances nanotechnology, particular, led development of nanomedicine designed specifically target CAFs, offering new possibilities for precise personalized therapies. This article reviews recent progress using nanocarriers that CAFs. Additionally, we explore potential combining multiple therapies, such chemotherapy immunotherapy, with enhance efficacy overcome resistance. Although many preclinical studies show promise, clinical application still faces considerable challenges, especially terms penetration large-scale production. Therefore, this review aims provide fresh perspective on CAF-targeted highlight future research directions applications.

Language: Английский

Citations

0
Bacteriophages as Targeted Therapeutic Vehicles: Challenges and Opportunities DOI Creative Commons

Srividhya Venkataraman,

Mehdi Shahgolzari,

Afagh Yavari

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(5), P. 469 - 469

Published: April 29, 2025

Bacteriophages, with their distinctive ability to selectively target host bacteria, stand out as a compelling tool in the realm of drug and gene delivery. Their assembly from proteins nucleic acids, coupled modifiable biologically unique properties, enables them serve efficient safe delivery systems. Unlike conventional nanocarriers, which face limitations such non-specific targeting, cytotoxicity, reduced transfection efficiency vivo, engineered phages exhibit promising potential overcome these hurdles improve outcomes. This review highlights bacteriophage-based systems innovative for delivering therapeutic agents. It explores strategies engineering bacteriophage, categorizes principal types employed delivery, evaluates applications disease therapy. provides intriguing details use natural therapy diseases cancer, bacterial viral infections, veterinary diseases, neurological disorders, well phage display technology generating monoclonal antibodies against various human diseases. Additionally, CRISPR-Cas9 genetically is elucidated. Furthermore, it critical analysis challenges associated phage-based systems, offering insights overcoming obstacles. By showcasing advancements integration into nanotechnology, this study underscores revolutionize approaches inspire future innovations medicine.

Language: Английский

Citations

0