Structural Stabilization of Clinically Oriented Oligomeric Proteins During their Transit through Synthetic Secretory Amyloids
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(21)
Published: March 19, 2024
Developing
time-sustained
drug
delivery
systems
is
a
main
goal
in
innovative
medicines.
Inspired
by
the
architecture
of
secretory
granules
from
mammalian
endocrine
system
it
has
generated
non-toxic
microscale
amyloid
materials
through
coordination
between
divalent
metals
and
poly-histidine
stretches.
Like
their
natural
counterparts
that
keep
functionalities
assembled
protein,
those
synthetic
structures
release
biologically
active
proteins
during
slow
self-disintegration
process
occurring
vitro
upon
vivo
administration.
Being
these
formed
single
pure
protein
species
therefore,
chemically
homogenous,
they
act
as
highly
promising
systems.
Despite
enormous
clinical
potential,
nature
clustering
quality
released
have
been
so
far
neglected
issues.
By
using
diverse
polypeptide
protein-only
oligomeric
nanoscale
versions
convenient
models,
conformational
rearrangement
stabilization
building
blocks
transit
granules,
being
material
structurally
distinguishable
original
source
proved
here.
This
fact
indicates
dynamic
amyloids
arrangers
rather
than
plain,
inert
protein-recruiting/protein-releasing
granular
depots.
Language: Английский
Surpassing protein specificity in biomimetics of bacterial amyloids
International Journal of Biological Macromolecules,
Journal Year:
2025,
Volume and Issue:
unknown, P. 139635 - 139635
Published: Jan. 1, 2025
Language: Английский
Lyophilization of biomimetic amyloids preserves their regulatable, endocrine-like functions for nanoparticle release
Applied Materials Today,
Journal Year:
2024,
Volume and Issue:
39, P. 102348 - 102348
Published: July 25, 2024
The
secretory
granules
from
the
mammalian
endocrine
system
are
functional
amyloids
that
act
as
dynamic
depots
to
store
and
release
protein
hormones
into
bloodstream.
controlled
in
vitro
coordination
between
divalent
cations
solvent-exposed
histidine
residues
triggers
reversible,
cross-molecular
interactions
result
granular
aggregates
with
protein-leaking
properties.
While
these
synthetic
particles
mechanically
stable,
they
progressively
disintegrate
their
building
blocks,
mimicking
performance
of
granules.
Envisaged
delivery
systems
for
endocrine-like,
time-sustained
release,
clinical
applicability
should
be
supported
by
robust
storage
procedures,
so
far
unset.
Being
lyophilization
a
desirable
method
drugs,
how
this
procedure
could
preserve
clinically
oriented
is
neglected
issue.
We
have
here
explored,
tailored
validated
an
industrially
friendly,
fully
scalable
approach
aimed
at
secretion
protein-only
nanoparticles.
By
doing
so,
protein-protein
such
materials
been
characterized,
citrate
identified
efficient
modulator
temporal
profile,
through
which
sustainability
leaking
process
can
finely
regulated.
Language: Английский
Investigation of the Mechanism of Action of AMPs from Amphibians to Identify Bacterial Protein Targets for Therapeutic Applications
Carolina Canè,
No information about this author
Lidia Tammaro,
No information about this author
Angela Duilio
No information about this author
et al.
Antibiotics,
Journal Year:
2024,
Volume and Issue:
13(11), P. 1076 - 1076
Published: Nov. 12, 2024
Antimicrobial
peptides
(AMPs)
from
amphibians
represent
a
promising
source
of
novel
antibacterial
agents
due
to
their
potent
and
broad-spectrum
antimicrobial
activity,
which
positions
them
as
valid
alternatives
conventional
antibiotics.
This
review
provides
comprehensive
analysis
the
mechanisms
through
amphibian-derived
AMPs
exert
effects
against
bacterial
pathogens.
We
focus
on
identification
protein
targets
implicated
in
action
these
biological
processes
altered
by
effect
AMPs.
By
examining
recent
advances
countering
multidrug-resistant
bacteria
multi-omics
approaches,
we
elucidate
how
interact
with
membranes,
enter
cells,
target
specific
protein.
discuss
implications
interactions
developing
targeted
therapies
overcoming
antibiotic
resistance
(ABR).
aims
integrate
current
knowledge
AMPs’
mechanisms,
identify
gaps
our
understanding,
propose
future
directions
for
research
harness
amphibian
clinical
applications.
Language: Английский
Hybrid Micro-/Nanoprotein Platform Provides Endocrine-like and Extracellular Matrix-like Cell Delivery of Growth Factors
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(26), P. 32930 - 32944
Published: June 18, 2024
Protein
materials
are
versatile
tools
in
diverse
biomedical
fields.
Among
them,
artificial
secretory
granules
(SGs),
mimicking
those
from
the
endocrine
system,
act
as
mechanically
stable
reservoirs
for
sustained
release
of
proteins
oligomeric
functional
nanoparticles.
Only
validated
oncology,
physicochemical
properties
SGs,
along
with
their
combined
drug-releasing
and
scaffolding
abilities,
make
them
suitable
smart
topographies
regenerative
medicine
prolonged
delivery
growth
factors
(GFs).
Thus,
considering
need
novel,
safe,
cost-effective
to
present
GFs,
this
study,
we
aimed
biofabricate
a
protein
platform
combining
both
endocrine-like
extracellular
matrix
fibronectin-derived
(ECM-FN)
systems.
This
approach
is
based
on
nanostructured
histidine-tagged
version
human
fibroblast
factor
2.
The
GF
presented
onto
polymeric
surfaces,
interacting
FN
spontaneously
generate
nanonetworks
that
absorb
solid
state,
modulate
mesenchymal
stromal
cell
(MSC)
behavior.
results
show
SGs-based
trigger
high
rates
MSCs
proliferation
while
preventing
differentiation.
While
could
be
useful
therapy
manufacture
demanding
large
numbers
unspecialized
MSCs,
it
fully
validates
hybrid
convenient
setup
design
biologically
active
surfaces
tissue
engineering
controlled
manipulation
mammalian
growth.
Language: Английский
Citrate-Assisted Regulation of Protein Stability and Secretability from Synthetic Amyloids
ACS Applied Materials & Interfaces,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 26, 2025
The
mammalian
endocrine
system
uses
functional
amyloids
as
dynamic
depots
to
store
and
release
protein
hormones
into
the
bloodstream.
Such
depots,
acting
secretory
granules
within
microscale,
are
formed
in
specialized
cells
by
coordination
between
ionic,
divalent
form
of
zinc
(Zn2+)
imidazole
ring
from
accessible
His
residues.
reversibility
such
cross-linking
events
allows
for
monomeric
or
oligomeric
forms
biological
activity.
In
vitro,
mimicking
a
natural
process,
synthetic
amyloidal
with
properties
can
be
fabricated
using
selected
therapeutic
proteins
building
blocks.
Then,
these
microparticles
act
delivery
systems
endocrine-like,
sustained
release,
proven
applicability
vaccinology,
cancer
therapy,
regenerative
medicine,
antimicrobial
agents.
While
temporal
profile
which
is
leaked
material
might
highly
relevant
clinically
oriented
applications,
fine
control
parameters
remains
unclear.
We
have
explored
here
how
kinetics
regulated
intervening
storage
formulation
granules,
through
concentration
citrate
not
only
buffer
component
stabilizer
but
also
chelating
agent.
citrate-assisted,
time-prolonged
regulatable
proteins,
their
form,
opens
spectrum
possibilities
adjust
preparation
specific
clinical
needs.
Language: Английский
Subcutaneous Administration of an Endocrine-Mimetic, Slow-Release Protein Material Reduces the Severity of SARS-CoV-2 Infection
Journal of Drug Delivery Science and Technology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 106813 - 106813
Published: March 1, 2025
Language: Английский