Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review DOI Creative Commons
Marcelly Santana Mascarenhas, Fernanda dos Santos Nascimento, Anelita de Jesus Rocha

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(10), P. 11086 - 11123

Published: Oct. 2, 2024

The objective of this systematic review (SR) was to select studies on the use gene editing by CRISPR technology related plant resistance biotic stresses. We sought evaluate articles deposited in six electronic databases, using pre-defined inclusion and exclusion criteria. This SR demonstrates that countries such as China United States America stand out with CRISPR/Cas. Among most studied crops are rice, tomatoes model

Language: Английский

GbPP2C80 Interacts with GbWAKL14 to Negatively Co‐Regulate Resistance to Fusarium and Verticillium wilt via MPK3 and ROS Signaling in Sea Island Cotton DOI Creative Commons
Nan Zhao, Anhui Guo, Weiran Wang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 18, 2024

Fusarium wilt (FW) is widespread in global cotton production, but the mechanism underlying FW resistance superior-fiber-quality Sea Island unclear. This study reveals that has been target of genetic improvement China since 2010s. The key nonsynonymous single nucleotide polymorphism (SNP, T/C) gene Gbar_D03G001670 encoding protein phosphatase 2C 80 (PP2C80) results an amino acid shift (L/S), which significantly associated with cotton. Silencing GbPP2C80 increases cotton, whereas overexpressing reduces Arabidopsis. and GbWAKL14 exist synergistically accessions haplotype forms "susceptible-susceptible" (TA) "resistant-resistant" (CC), interact each other. CRISPR/Cas9-mediated knockout enhances Verticillium (VW) upland overexpression weakens VW respond to by modulating reactive oxygen species (ROS) content via affecting MPK3 expression. In summary, two tandem genes on chromosome D03, GbPP2C80, GbWAKL14, functions as cooperative negative regulators disease defense, providing novel resources molecular markers for development resistant cultivars.

Language: Английский

Citations

4
Recognition of a Fungal Effector Potentiates Pathogen‐Associated Molecular Pattern‐Triggered Immunity in Cotton DOI Creative Commons
Lifan Sun, Xiang‐Guo Li,

Jiajie Zhong

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract Plants are equipped with multi‐layered immune systems that recognize pathogen‐derived elicitors to activate immunity. Verticillium dahliae is a soil‐borne fungus infects broad range of plants and causes devastating wilt disease. The mechanisms underlying recognition between V. remain elusive. Here, secretory protein, elicitor plant defense gene ( VdEPD1 ), acts as an triggers responses in both Nicotiana benthamiana cotton identified. Targeted deletion enhances virulence plants. Expression the accumulation reactive oxygen species (ROS) activation cell death Gossypium barbadense EPD1‐interacting receptor‐like cytoplasmic kinase (GbEIR5A) GbEIR5D interact VdEPD1. Silencing GbEIR5A/D significantly impairs VdEPD1‐triggered plants, indicating contribution VdEPD1‐activated effector‐triggered immunity (ETI). stimulates expression GbEIR5A Interestingly, silenced genes exhibit compromised pathogen‐associated molecular patterns (PAMPs)‐triggered ROS accumulation, whereas overexpression or PAMP‐induced ROS. These findings indicate potentiates GbEIRs enhance PAMP‐triggered (PTI), uncovering cooperative interplay PTI ETI cotton.

Language: Английский

Citations

0
Integrated Transcriptomic and Metabolomic Analysis of G. hirsutum and G. barbadense Responses to Verticillium Wilt Infection DOI Open Access

Baoguang Xing,

Pengtao Li,

Yanfang Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 28 - 28

Published: Dec. 24, 2024

Verticillium wilt (VW) caused by dahliae (Vd) is a devastating fungal cotton disease characterized high pathogenicity, widespread distribution, and frequent variation. It leads to significant losses in both the yield quality of cotton. Identifying key non-synonymous single nucleotide polymorphism (SNP) markers crucial genes associated with VW resistance Gossypium hirsutum barbadense, subsequently breeding new disease-resistant varieties, are essential for management. Here, we sequenced transcriptome metabolome roots TM-1 (G. hirsutum) Hai7124 barbadense) after 0, 1, 2 days V991 inoculation. Transcriptome analysis identified total 72,752 genes, 5814 differentially expressed (DEGs) determined through multiple group comparisons. KEGG enrichment revealed that pathways enriched DEGs obtained from longitudinal transverse comparisons contained glutathione metabolism pathway. Metabolome 995 metabolites, 22 accumulated metabolites (DAMs), which were correlated including metabolism, degradation valine, leucine, isoleucine, biosynthesis terpenoids, alkaloids, pyridine, piperidine. The conjoint transcriptomic metabolomic sequencing DAMs pathway, candidate gene GH_D11G2329 (glutathione S-transferase, GSTF8) potentially response infection was selected. These findings establish basis investigating mechanisms underlying plant’s VW.

Language: Английский

Citations

0
Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review DOI Creative Commons
Marcelly Santana Mascarenhas, Fernanda dos Santos Nascimento, Anelita de Jesus Rocha

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(10), P. 11086 - 11123

Published: Oct. 2, 2024

The objective of this systematic review (SR) was to select studies on the use gene editing by CRISPR technology related plant resistance biotic stresses. We sought evaluate articles deposited in six electronic databases, using pre-defined inclusion and exclusion criteria. This SR demonstrates that countries such as China United States America stand out with CRISPR/Cas. Among most studied crops are rice, tomatoes model

Language: Английский

Citations

0