Addressing Heterogeneity in Direct Analysis of Extracellular Vesicles and Their Analogs by Membrane Sensing Peptides as Pan‐Vesicular Affinity Probes
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(29)
Published: May 31, 2024
Abstract
Extracellular
vesicles
(EVs),
crucial
mediators
of
cell‐to‐cell
communication,
hold
significant
diagnostic
potential
due
to
their
ability
concentrate
protein
biomarkers
in
bodily
fluids.
However,
challenges
isolating
EVs
from
biological
specimens
hinder
widespread
use.
The
preferred
strategy
involves
direct
analysis,
integrating
isolation
and
analysis
solutions,
with
immunoaffinity
methods
currently
dominating.
Yet,
the
heterogeneous
nature
poses
challenges,
as
proposed
markers
may
not
be
universally
present
thought,
raising
concerns
about
biomarker
screening
reliability.
This
issue
extends
EV‐mimics,
where
conventional
lack
applicability.
Addressing
these
study
reports
on
Membrane
Sensing
Peptides
(MSP)
pan‐vesicular
affinity
ligands
for
both
non‐canonical
analogs,
streamlining
capture
phenotyping
through
Single
Molecule
Array
(SiMoA).
MSP
enable
circulating
EVs,
eliminating
need
prior
isolation.
Demonstrating
clinical
translation,
technology
detects
an
EV‐associated
epitope
signature
serum
plasma,
distinguishing
myocardial
infarction
stable
angina.
Additionally,
allow
tetraspanin‐lacking
Red
Blood
Cell‐derived
overcoming
limitations
associated
antibody‐based
methods.
Overall,
work
underlines
value
complementary
tools
antibodies,
advancing
EV
diagnostics
beyond,
marking
first‐ever
peptide‐based
application
SiMoA
technology.
Language: Английский
Isolation and detection strategies for decoding the heterogeneity of extracellular vesicles
Chemical Engineering Journal,
Journal Year:
2025,
Volume and Issue:
unknown, P. 160234 - 160234
Published: Feb. 1, 2025
Language: Английский
Microfluidic Innovations for Enhanced Extracellular Vesicle Isolation and Analysis: A Comprehensive Review
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 28, 2025
Language: Английский
Design and Optimization of a Silicon-Based Electrokinetic Microchip for Sensitive Detection of Small Extracellular Vesicles
ACS Sensors,
Journal Year:
2024,
Volume and Issue:
9(6), P. 2935 - 2945
Published: June 7, 2024
Detection
of
analytes
using
streaming
current
has
previously
been
explored
both
experimental
approaches
and
theoretical
analyses
such
data.
However,
further
developments
are
needed
for
establishing
a
viable
microchip
that
can
be
exploited
to
deliver
sensitive,
robust,
scalable
biosensor
device.
In
this
study,
we
demonstrated
the
fabrication
device
on
silicon
wafer
microfabrication
technology
followed
by
characterization
optimization
sensor
detection
small
extracellular
vesicles
(sEVs)
with
sizes
in
range
30
200
nm,
as
determined
nanoparticle
tracking
analyses.
We
showed
sensitivity
devices,
assessed
common
protein-ligand
pair
sEVs,
significantly
outperforms
previous
same
principle.
Two
versions
microchips,
denoted
enclosed
removable-top
were
developed
compared,
aiming
discern
importance
high-pressure
measurement
versus
easier
better
surface
preparation
capacity.
A
custom-built
chip
manifold
allowing
easy
interfacing
standard
microfluidic
connections
was
also
constructed.
By
investigating
different
electrical,
fluidic,
morphological,
fluorescence
measurements,
show
while
its
robust
glass-silicon
bonding
withstand
higher
pressure
thus
generate
current,
configuration
offers
several
practical
benefits,
including
preparation,
uniform
probe
conjugation,
improvement
limit
(LoD).
compared
two
functionalization
strategies
achieve
high
membrane
protein
profiling
low
LoD
sEV
detection.
At
optimum
working
condition,
could
detect
sEVs
reaching
an
10
Language: Английский
Emerging phospholipid-targeted affinity materials for extracellular vesicle isolation and molecular profiling
Journal of Chromatography A,
Journal Year:
2024,
Volume and Issue:
1741, P. 465639 - 465639
Published: Dec. 28, 2024
Language: Английский
Extracellular vesicle analytical science loses a touch of creativity and kindness
Journal of Extracellular Vesicles,
Journal Year:
2024,
Volume and Issue:
13(9)
Published: Sept. 1, 2024
Language: Английский
Age- and sex-related variations in extracellular vesicle profiling for the assessment of cardiovascular risk: the EVaging index
npj Aging,
Journal Year:
2024,
Volume and Issue:
10(1)
Published: Dec. 19, 2024
Abstract
Extracellular
vesicles
(EVs)
offer
valuable
diagnostic
and
prognostic
insights
for
cardiovascular
(CV)
diseases,
but
the
influence
of
age-related
chronic
inflammation
(“inflammaging”)
sex
differences
on
EV
profiles
linked
to
CV
risk
remains
unclear.
This
study
aimed
use
profiling
predict
age
stratify
patients
by
risk.
We
developed
an
EVaging
index
analyzing
surface
antigen
serum
EVs
from
625
participants,
aged
20
94
years,
across
varying
groups.
The
was
associated
with
in
healthy
individuals
distinguished
patients,
correlating
outcomes
likelihood
fatal
events
according
European
Society
Cardiology
(ESC)
SCORE,
reflecting
age-associated
comorbidities.
While
changes
disease-related
fingerprint
adds
complexity
may
help
assess
biological
aging
risk,
emphasizing
EVs’
roles
inflammaging.
Language: Английский