Recent advances in brain organoids: a comprehensive review of the last eight years DOI
Yuan Xiong, Ting‐Hua Wang

New discovery., Journal Year: 2024, Volume and Issue: unknown, P. 1 - 14

Published: Oct. 31, 2024

Organoids are three-dimensional cellular structures grown in vitro that can self-organize and differentiate into cell types with organ-specific functions, closely mimicking the biological properties of tissues organs vivo. Brain organoids, which resembling brain function, serve as valuable models for medical research, including disease microenvironment simulation, mechanism exploration, drug evaluation. In this review, we analyzed 808 articles retrieved from PubMed, CNKI, Wanfang databases using keyword "brain organoids," 180 were included. We summarized research progress organoids over past eight years by categorizing refining findings. Our analysis shows have achieved significant success simulating development vitro, leading to establishment refinement 3D organoid research. been widely applied explore disease-related mechanisms, yielding promising results opening avenues further on human brain. summarize three areas: culture methods, exploration.

Language: Английский

Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids DOI Creative Commons
Hye Sung Kim, Yang Xiao, Xuejing Chen

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(21)

Published: March 28, 2024

Understanding the impact of long-term opioid exposure on embryonic brain is critical due to surging number pregnant mothers with dependency. However, this has been limited by human inaccessibility and cross-species differences in animal models. Here, a midbrain model established that uses hiPSC-derived organoids assess cell-type-specific responses acute chronic fentanyl treatment withdrawal. Single-cell mRNA sequencing 25,510 cells from different groups reveals arrests neuronal subtype specification during early development alters synaptic activity neuron projection. In contrast, increases dopamine release but does not significantly alter gene expression related cell lineage development. These results provide first examination effects at single-cell level.

Language: Английский

Citations

7
Investigating the neurobiology of maternal opioid use disorder and prenatal opioid exposure using brain organoid technology DOI Creative Commons

Ila Dwivedi,

Gabriel G. Haddad

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: May 15, 2024

Over the past two decades, Opioid Use Disorder (OUD) among pregnant women has become a major global public health concern. OUD been characterized as problematic pattern of opioid use despite adverse physical, psychological, behavioral, and or social consequences. Due to relapsing–remitting nature this disorder, mothers are chronically exposed exogenous opioids, resulting in neurological neuropsychiatric outcomes. Collateral fetal exposure opioids also precipitates severe neurodevelopmental neurocognitive sequelae. At present, much what is known regarding neurobiological consequences prenatal (POE) derived from preclinical studies animal models postnatal postmortem investigations humans. However, species-specific differences brain development, variations subject age/health/background, disparities sample collection storage have complicated interpretation findings produced by these explorations. The ethical logistical inaccessibility human tissue limited direct examinations drug effects. To circumvent confounding factors, recent groups begun employing induced pluripotent stem cell (iPSC)-derived organoid technology, which provides access key aspects cellular molecular structure, function vitro . In review, we endeavor encapsulate advancements culture that enabled scientists model dissect neural underpinnings effects POE. We hope not only emphasize utility organoids for investigating conditions, but highlight opportunities further technical conceptual progress. Although application critical field research still its nascent stages, understanding neurobiology POE via modality will provide insights improving maternal

Language: Английский

Citations

4
Cocaine perturbs neurodevelopment and increases neuroinflammation in a prenatal cerebral organoid model DOI Creative Commons
Jessica L. Davis, Ciarán Kennedy, C. McMahon

et al.

Translational Psychiatry, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 26, 2025

Prenatal exposure to cocaine causes abnormalities in foetal brain development, which are linked later development of anxiety, depression and cognitive dysfunction. Previous studies rodent models have indicated that prenatal affects proliferation, differentiation connectivity neural cell types. Here, using cerebral organoids derived from the human iPSC line HPSI1213i-babk_2, we investigated cocaine-induced changes gene expression regulatory landscape at an early developmental time point, leveraging recent advances single RNA-seq ATAC-seq. iPSC-cerebral replicated well-established responses observed vivo provided additional information about cell-type specific regulation following exposure. Cocaine altered patterns, part through epigenetic remodelling, revealed disordered plasticity mechanisms organoids. Perturbed neurodevelopmental cellular signalling inflammatory-like activation astrocyte populations were also evident The combination neuroplasticity, neurodevelopment neuroinflammatory suggests can mediate substantial disruption normal maturation brain. These findings offer new insights into mechanism underlying adverse effects on point possible pathomechanisms neuropsychiatric disturbances.

Language: Английский

Citations

0
A human iPSC-derived midbrain neural stem cell model of prenatal opioid exposure and withdrawal: A proof of concept study DOI Creative Commons
Rhea Sullivan,

Quinn Ahrens,

Sara Mills‐Huffnagle

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0319418 - e0319418

Published: April 1, 2025

A growing body of clinical literature has described neurodevelopmental delays in infants with chronic prenatal opioid exposure and withdrawal. Despite this, the mechanism how opioids impact developing brain remains unknown. Here, we developed an vitro model morphine withdrawal using healthy human induced pluripotent stem cell (iPSC)-derived midbrain neural progenitors monolayer. To optimize our model, identified that a longer induction regional patterning period increases expression canonical receptors mu kappa compared to shorter protocol ( OPRM1 , two-tailed t-test, p = 0.004; OPRK1 0.0003). Next, showed derived from iPSC also have scant toll-like receptor 4 (TLR4) expression, key player neonatal syndrome pathophysiology. During withdrawal, differentiating experience cyclic adenosine monophosphate overshoot exposed vehicle 0.0496) conditions 0.0136, 1-way ANOVA). Finally, alters proportions differentiated progenitor fates (2-way ANOVA, F 16.05, < 0.0001). Chronic increased nestin positive 0.0094), decreased neuronal nuclear antigen neurons (NEUN) 0.0047) those vehicle. Morphine glial fibrillary acidic protein cells astrocytic lineage 0.044), NEUN-positive 0.0001) only. Applications this paradigm include mechanistic studies underscoring fate commitments early neurodevelopment during

Language: Английский

Citations

0
Understanding the impact of the gut microbiome on opioid use disorder: Pathways, mechanisms, and treatment insights DOI Creative Commons
Negin Kazemian, Sepideh Pakpour

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(10)

Published: Oct. 1, 2024

The widespread use of opioids for chronic pain management not only poses a significant public health issue but also contributes to the risk tolerance, dependence, and addiction, leading opioid disorder (OUD), which affects millions globally each year. Recent research has highlighted potential bidirectional relationship between gut microbiome OUD. This emerging perspective is critical, especially as epidemic intensifies, emphasizing need investigate how OUD may alter dynamics vice versa. Understanding these interactions could reveal new insights into mechanisms addiction well provide novel approaches managing potentially mitigating impacts. comprehensive review explores intricate link through gut-brain axis, focusing on opiates influence microbial composition, functional changes, mucosal integrity. By synthesizing current findings, aims inspire strategies combat crisis leverage microbiome-centred interventions preventing treating

Language: Английский

Citations

2
Unraveling the Effects of Prenatal Anesthesia on Neurodevelopment: A Review of Current Evidence and Future Directions DOI
Qiu‐Xia Xiao,

Min‐Jian Geng,

Qiulin Wang

et al.

NeuroToxicology, Journal Year: 2024, Volume and Issue: 105, P. 96 - 110

Published: Sept. 12, 2024

Language: Английский

Citations

0
Recent advances in brain organoids: a comprehensive review of the last eight years DOI
Yuan Xiong, Ting‐Hua Wang

New discovery., Journal Year: 2024, Volume and Issue: unknown, P. 1 - 14

Published: Oct. 31, 2024

Organoids are three-dimensional cellular structures grown in vitro that can self-organize and differentiate into cell types with organ-specific functions, closely mimicking the biological properties of tissues organs vivo. Brain organoids, which resembling brain function, serve as valuable models for medical research, including disease microenvironment simulation, mechanism exploration, drug evaluation. In this review, we analyzed 808 articles retrieved from PubMed, CNKI, Wanfang databases using keyword "brain organoids," 180 were included. We summarized research progress organoids over past eight years by categorizing refining findings. Our analysis shows have achieved significant success simulating development vitro, leading to establishment refinement 3D organoid research. been widely applied explore disease-related mechanisms, yielding promising results opening avenues further on human brain. summarize three areas: culture methods, exploration.

Language: Английский

Citations

0