Research progress of nano drug delivery systems in the anti-tumor treatment of traditional Chinese medicine monomers DOI Creative Commons
Bocui Song, Shuang Li, Shuang Zhang

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19332 - e19332

Published: April 24, 2025

Tumors pose a serious threat to global public health and are usually treated from two aspects: tumor cells microenvironment. Compared with traditional chemotherapy drugs, Chinese medicine (TCM) monomers have advantages in treatment, such as multiple targets, levels synergistic intervention. However, most TCM active ingredients disadvantages poor water solubility stability, which restrict their clinical application. Nano drug delivery systems the functions of improving bioavailability anti-tumor ingredients, enhancing tissue targeting, achieving controlled release, inhibiting multidrug resistance. free monomers, they higher therapeutic effects fewer side effects. This article summarizes five commonly used monomer nanocarriers, including lipid nanomaterials, exosomes, polymer micelles, carbon nanotubes, dendrimers, explains mechanisms after combining TCM, cell proliferation metastasis, regulating microenvironment, etc . At same time, potential nano combined radiotherapy immunotherapy is discussed, well current problems toxicity, long-term complex amplification process, future development directions, aiming provide reference for promoting application ingredients.

Language: Английский

Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

Citations

42
Platelet-derived extracellular vesicles: emerging players in hemostasis and thrombosis DOI

Aziz Kubaev,

Fadhil Faez Sead, Mohammad Pirouzbakht

et al.

Journal of Liposome Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: April 25, 2025

Platelets, long recognized for their role in hemostasis and thrombosis, have emerged as key players a wide array of physiological pathological processes through the release platelet-derived extracellular vesicles (PEVs). These nanoscale vesicles, rich bioactive molecules such proteins, lipids, nucleic acids, facilitate intercellular communication influence ranging from angiogenesis inflammation to immune modulation tissue repair. PEVs, most abundant circulation, display procoagulant activity 50-100 times greater than activated platelets, underscoring pivotal thrombosis. Recent research has unveiled dual health disease, highlighting potential diagnostic biomarkers therapeutic vehicles. PEVs are implicated cancer progression, autoimmune diseases, infectious where they modulate tumor microenvironments, responses, inflammatory pathways. Moreover, ability deliver agents with high specificity biocompatibility positions them promising tools regenerative medicine, drug delivery, targeted therapies. This review comprehensively explores PEV biogenesis, cargo composition, multifaceted roles well broader implications disease. It also markers innovative strategies, offering insights into application treating thrombotic disorders, cancer, diseases. Despite significant advancements, challenges remain standardizing isolation protocols translating preclinical findings clinical applications. Unlocking full promises revolutionize diagnostics therapeutics, paving way novel approaches managing complex

Language: Английский

Citations

4
Effect of Extracellular Vesicles Derived From Tumor Cells on Immune Evasion DOI Creative Commons

Xixi Liu,

Kenneth K.W. To,

Qinsong Zeng

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

The crosstalk between immunity and cancer in the regulation of tumor growth is considered a hallmark cancer. Antitumor refers to innate adaptive immune responses that regulate development proliferation. Tumor evasion represents major hindrance effective anticancer treatment. Extracellular vesicles (EVs) are nano-sized lipid-bilayer-enclosed particles secreted extracellular space by all cell types. They critically involved numerous biological functions including intercellular communication. Tumor-derived (TEVs) can transport variety cargo modulate cells microenvironment (TME). This review provides latest update about how evade surveillance exploiting TEVs. First, biogenesis EVs cargo-sorting machinery discussed. Second, differentiation, activation, function via TEVs illustrated. Last but not least, novel antitumor strategies reverse escape summarized.

Language: Английский

Citations

3
Nanoscopic Profiling of Small Extracellular Vesicles via High‐Speed Atomic Force Microscopy (HS‐AFM) Videography DOI Creative Commons

Muhammad Isman Sandira,

Keesiang Lim, Takeshi Yoshida

et al.

Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(4)

Published: March 26, 2025

Small extracellular vesicles (sEVs), which carry lipids, proteins and RNAs from their parent cells, serve as biomarkers for specific cell types biological states. These vesicles, including exosomes microvesicles, facilitate intercellular communication by transferring cellular components between cells. Current methods, such ultracentrifugation Tim-4 affinity method, yield high-purity sEVs. However, despite small size, purified sEVs remain heterogeneous due to varied intracellular origins. In this technical note, we used high-speed atomic force microscopy (HS-AFM) in conjunction with exosome markers (IgGCD63 IgGCD81) explore the origins of at single-sEV resolution. Our results first revealed nanotopology HEK293T-derived under physiological conditions. Larger (diameter > 100 nm) exhibited greater height fluctuations compared smaller ≤ nm). Next, found that mouse-origin IgGCD63, rabbit-origin IgGcontrol IgGCD81, iconic 'Y' conformation, similar structural dynamics properties. Last, marker antibodies predominantly co-localised sEVd nm but not nm, demonstrating CD63-CD81-enriched sEV CD63-CD81-depleted subpopulations. summary, demonstrate nanoscopic profiling surface on using HS-AFM is feasible characterising distinct subpopulations a mixture.

Language: Английский

Citations

3
Exosome: an overview on enhanced biogenesis by small molecules DOI

Amir Bavafa,

Maryam Izadpanahi,

Elham Hosseini

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 25, 2025

Language: Английский

Citations

1
Emerging Frontiers in acute kidney injury: The role of extracellular vesicles DOI
Sirui Li, Lan Zhou, Yu Huang

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 149 - 170

Published: Feb. 18, 2025

Language: Английский

Citations

1
Biosensor-based methods for exosome detection with applications to disease diagnosis DOI

Weikang Ge,

Zheying Mu,

Shiao Yang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117362 - 117362

Published: March 1, 2025

Language: Английский

Citations

1
Small extracellular vesicles: the origins, current status, future prospects, and applications DOI Creative Commons
Xinyi Zhou, Jin Huang,

Dianqi Zhang

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 17, 2025

Small extracellular vesicles (sEVs) are membrane-bound with a size of less than 200 nm, released by cells. Due to their relatively small molecular weight and ability participate in intercellular communication, sEVs can serve not only as carriers biomarkers for disease diagnosis but also effective drug delivery agents. Furthermore, these involved regulating the onset progression various diseases, reflecting physiological functional states This paper introduces classification vesicles, focus on extraction identification significant role repair, diagnosis, communication. Additionally, addresses engineering modification provide reference enhanced understanding application.

Language: Английский

Citations

1
Toward Clarity in Single Extracellular Vesicle Research: Defining the Field and Correcting Missteps DOI Creative Commons
Yun Su,

Wanzhuo He,

Lei Zheng

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Single extracellular vesicle (EV) research holds the potential to revolutionize our understanding of cellular communication and enable breakthroughs in diagnostics therapeutics. However, lack a clear, consensus-driven definition single EV has led methodological inconsistencies, overgeneralized interpretations, and, some cases, misleading claims. In this perspective, we propose framework for defining research, critique current challenges misconceptions field, discuss its implications biomedical applications. We argue that precise experimental design, rigorous validation, interdisciplinary collaboration approaches are needed establish as cornerstone precision medicine.

Language: Английский

Citations

1
Harnessing engineered extracellular vesicles for enhanced therapeutic efficacy: advancements in cancer immunotherapy DOI Creative Commons
Zheng Gong, Cheng Cheng, Chaonan Sun

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2025, Volume and Issue: 44(1)

Published: May 2, 2025

Abstract Extracellular vesicles (EVs), particularly engineered variants, have emerged as promising tools in cancer immunotherapy due to their inherent ability modulate immune responses and deliver therapeutic agents with high specificity minimal toxicity. These nanometer-sized vesicles, which include exosomes (Exos) other subtypes, naturally participate intercellular communication are capable of carrying a diverse range bioactive molecules, including proteins, lipids, RNAs, metabolites. Recent advancements the biogenesis EVs, such strategies modify surface characteristics cargo, significantly expanded potential effective vehicles for targeted therapies. Tailoring contents incorporating immunomodulatory molecules or gene-editing (GETs), has shown outcomes enhancing anti-tumor immunity overcoming immunosuppressive tumor microenvironment (TME). Moreover, optimizing delivery mechanisms, through both passive active targeting strategies, is crucial improving clinical efficacy EV-based This review provides an overview recent developments engineering EVs immunotherapy, focusing on biogenesis, methods content customization, innovations cargo delivery. Additionally, addresses challenges associated translation therapies, issues related scalability, safety, By offering insights into current state field identifying key areas future research, this aims advance application treatment.

Language: Английский

Citations

1