TaDL interacts with TaB3 and TaNF‐YB1 to synergistically regulate the starch synthesis and grain quality in bread wheat DOI Creative Commons

Guoyu Liu,

Runqi Zhang, Zhenlong Wu

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

ABSTRACT Starch biosynthesis is a critical factor in wheat ( Triticum aestivum L.) quality and yield. However, the full scope of its regulation not fully understood. Here we report that TaDL interacts with TaB3 TaNF‐YB1 to synergistically regulate starch wheat. Genome‐edited tadl mutant lines had smaller lighter grains lower total amylose contents compared wild type (WT). Correspondingly, transcript levels biosynthesis‐related genes, including TaSUS1 , TaSUS2 TaAGPL2 TaSBEIIa TaGBSSII TaSWEET2a were markedly at 15 d after flowering (DAF) mutants. physically interacted activated transcription through direct binding their promoter regions. A null also affected grain filling, phenotypes similar those mutants, whereas overexpression promoted filling. Our study demonstrated plays an essential role identified elite allele TaDL‐BI ) associated content, providing insights into underlying molecular mechanism which may be useful breeding high‐yielding improvement.

Language: Английский

Integrative Omics reveals genetic basis and TaMYB7-A1's function in wheat WUE and drought resilience DOI Creative Commons
Jun Xiao, Yuxin Zhou, H. M. Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Improving water use efficiency (WUE) and drought resistance in wheat is critical for ensuring global food security under changing climate conditions. Here, we integrated multi-omic data, including population-scale phenotyping, transcriptomics, genomics, to dissect the genetic molecular mechanisms underlying WUE resilience wheat. Genome-wide association studies (GWAS) revealed 8,135 SNPs associated with WUE-related traits, identifying 258 conditional non-conditional QTLs, many of which co-localized known drought-resistance genes. Pan-transcriptome analysis uncovered tissue-specific expression patterns, core unique gene functions, dynamic sub-genomic biases response drought. eQTL mapping pinpointed 146,966 regulatory loci, condition-specific hotspots enriched genes involved regulation, osmoregulation, photosynthesis. Integration Weighted co-expression network (WGCNA), Summary-data-based Mendelian Randomization (SMR) GWAS, eQTLs identified 207 candidate causal as key regulators traits wheat, such TaMYB7-A1. Functional analyses found that TaMYB7-A1 enhances tolerance by promoting root growth, reducing oxidative stress, improving osmotic enabling better access survival stress. It also increases photosynthesis WUE, boosting yield without compromising performance well-watered conditions, making it ideal target breeding. Our findings provide a comprehensive omic framework understanding architecture resistance, offering valuable targets breeding resilient varieties.

Language: Английский

Citations

0
Reshaping epigenomic landscapes in facilitating the speciation of bread wheat DOI Creative Commons
Zhaoheng Zhang, Xuelei Lin, Jingjing Yue

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Summary Polyploidization is a driving force of wheat evolution and speciation, yet its impact on epigenetic regulation gene expression remains unclear. Here, we constructed high-resolution landscape across leaves, spikes, roots hexaploidy tetraploid diploid relatives. Inter-species stable-expression genes exhibited conserved amino acid sequences under strong purifying selection, while dynamic-expression were linked to species-specific adaptation. During hexaploidization, dominant D-subgenome homoeolog was suppressed via reduced activating signals, converging with the A B subgenomes. Proximal chromatin regions near more stable, whereas distal regions, particularly enhancer-like elements mediated by H3K27ac H3K4me3, exhibit higher dynamism. Sequence variations in these enhancers lead differential regulation, influencing traits such as spike development. For instance, two haplotypes dCRE region TaDEP-B1 resulted significant differences spikelet numbers. We also observed coevolution transcription factors their binding sites, within expanded ERF family, which regulates morphology. This study highlights interplay between sequence variation modifications shaping transcriptional during offering valuable insights for genetic improvement.

Language: Английский

Citations

0
Integrated Transcriptomic and Proteomic Analyses Revealed Molecular Mechanism Underlying Nutritional Changes During Seed Development of Chenopodium Quinoa DOI
Nazer Manzoor,

Jiahong Yuan,

Wenhua Dongcheng

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
TaDL interacts with TaB3 and TaNF‐YB1 to synergistically regulate the starch synthesis and grain quality in bread wheat DOI Creative Commons

Guoyu Liu,

Runqi Zhang, Zhenlong Wu

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

ABSTRACT Starch biosynthesis is a critical factor in wheat ( Triticum aestivum L.) quality and yield. However, the full scope of its regulation not fully understood. Here we report that TaDL interacts with TaB3 TaNF‐YB1 to synergistically regulate starch wheat. Genome‐edited tadl mutant lines had smaller lighter grains lower total amylose contents compared wild type (WT). Correspondingly, transcript levels biosynthesis‐related genes, including TaSUS1 , TaSUS2 TaAGPL2 TaSBEIIa TaGBSSII TaSWEET2a were markedly at 15 d after flowering (DAF) mutants. physically interacted activated transcription through direct binding their promoter regions. A null also affected grain filling, phenotypes similar those mutants, whereas overexpression promoted filling. Our study demonstrated plays an essential role identified elite allele TaDL‐BI ) associated content, providing insights into underlying molecular mechanism which may be useful breeding high‐yielding improvement.

Language: Английский

Citations

0