Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 1, 2024
Language: Английский
The EMBO Journal, Journal Year: 2024, Volume and Issue: 43(11), P. 2127 - 2165
Published: April 5, 2024
The in vitro oxygen microenvironment profoundly affects the capacity of cell cultures to model physiological and pathophysiological states. Cell culture is often considered be hyperoxic, but pericellular levels, which are affected by diffusivity consumption, rarely reported. Here, we provide evidence that several types actually experience local hypoxia, with important implications for metabolism function. We focused initially on adipocytes, as adipose tissue hypoxia frequently observed obesity precedes diminished adipocyte Under standard conditions, cultured adipocytes highly glycolytic exhibit a transcriptional profile indicative hypoxia. Increasing diverted glucose flux toward mitochondria, lowered HIF1α activity, resulted widespread rewiring. Functionally, increased adipokine secretion sensitivity insulin lipolytic stimuli, recapitulating healthier model. functional benefits increasing were also macrophages, hPSC-derived hepatocytes cardiac organoids. Our findings demonstrate limiting many terminally-differentiated types, considering improves quality, reproducibility translatability models.
Language: Английский
Citations
14
Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)
Published: June 14, 2024
In oxygen (O
Language: Английский
Citations
7
Cancer Communications, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 3, 2025
Abstract Despite significant advancements in cancer treatment, current therapies often fail to completely eradicate malignant cells. This shortfall underscores the urgent need explore alternative approaches such as vaccines. Leveraging immune system's natural ability target and kill cells holds great therapeutic potential. However, development of vaccines is hindered by several challenges, including low stability, inadequate response activation, immunosuppressive tumor microenvironment, which limit their efficacy. Recent progress various fields, click chemistry, nanotechnology, exosome engineering, neoantigen design, offer innovative solutions these challenges. These achievements have led emergence smart vaccine platforms (SVPs), integrate protective carriers for messenger ribonucleic acid (mRNA) with functionalization strategies optimize targeted delivery. Click chemistry further enhances SVP performance improving encapsulation mRNA antigens facilitating precise delivery review highlights latest developments technologies therapy, exploring both opportunities challenges advancing transformative approaches.
Language: Английский
Citations
0
Medical Oncology, Journal Year: 2024, Volume and Issue: 41(11)
Published: Oct. 14, 2024
Language: Английский
Citations
2
Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 226, P. 279 - 287
Published: Nov. 20, 2024
Language: Английский
Citations
1
AJP Cell Physiology, Journal Year: 2024, Volume and Issue: 327(4), P. C959 - C978
Published: Aug. 26, 2024
The tumor microenvironment is complex and dynamic, characterized by poor vascularization, limited nutrient availability, hypoxia, an acidic pH. This environment plays a critical role in driving cancer progression. However, standard cell culture conditions used to study biology vitro fail replicate the vivo of tumors. Recently, "physiological" media that closely resemble human plasma have been developed (e.g., Plasmax, HPLM), along with more frequent adoption physiological oxygen (1%-8% O
Language: Английский
Citations
1
Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 1, 2024
Language: Английский
Citations
1