Molecular Imprinting of Phospholipids for Targeted Cell and Exosome Recognition

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Phospholipids, integral to the composition of cell membranes and extracellular vesicles, play a pivotal role in numerous biological processes. The precise identification, localization, isolation these membrane phospholipids are crucial for sophisticated imaging liquid biopsy. However, structural diversity amphiphilic nature present significant challenges. In this study, we have developed novel molecular imprinting strategy that integrates reversed-phase microemulsions with phosphoryl group-directed epitopes prepare molecularly imprinted polymers (MIPs). Titanium dioxide is employed as core material orient groups, thereby facilitating efficient alignment hydrophilic polar heads hydrophobic fatty acid tails at oil-water interface enabling specific phospholipids. Utilizing various types template molecules, including phosphatidylserine (PS), sphingomyelin (SM), phosphatidylethanolamine (PE), phosphatidylcholine (PC), synthesized MIPs exhibit high efficiency specificity recognition. These hold great potential selective recognition plasma membranes, offering an innovative detection low-abundance, Furthermore, PS-MIP demonstrates targeting particular tumor cells, making it suitable targeted drug delivery. application phospholipid-imprinted enables capture exosomes from body fluids, analysis lipid metabolites via mass spectrometry liver disease samples stages disease. This approach holds promise wide range applications exosome-based biopsies, method early monitoring diseases.

Language: Английский

Optogenetic Strategies for Optimizing the Performance of Phospholipids Biosensors

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: July 29, 2024

High-performance biosensors play a crucial role in elucidating the intricate spatiotemporal regulatory roles and dynamics of membrane phospholipids. However, enhancing sensitivity imaging performance remains significant challenge. Here, optogenetic-based strategies are presented to optimize phospholipid biosensors. These involves presequestering unbound cell nucleus regulating their cytosolic levels with blue light minimize background signal interference detection, particularly under conditions high expression biosensor. Furthermore, optically controlled phase separation SunTag system employed generate punctate probes for substrate thereby amplifying biosensor signals visualization detection process. improved hold great potential understanding lipids live cells methodological insights this study might be valuable developing other high-performance

Language: Английский