Physiochemically and Genetically Engineered Bacteria: Instructive Design Principles and Diverse Applications
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(30)
Published: June 12, 2024
With
the
comprehensive
understanding
of
microorganisms
and
rapid
advances
physiochemical
engineering
bioengineering
technologies,
scientists
are
advancing
rationally-engineered
bacteria
as
emerging
drugs
for
treating
various
diseases
in
clinical
disease
management.
Engineered
specifically
refer
to
advanced
or
genetic
technologies
combination
with
cutting
edge
nanotechnology
physical
which
have
been
validated
play
significant
roles
lysing
tumors,
regulating
immunity,
influencing
metabolic
pathways,
etc.
However,
there
has
no
specific
reviews
that
concurrently
cover
physiochemically-
genetically-engineered
their
derivatives
yet,
let
alone
distinctive
design
principles
functions
applications.
Herein,
applications
physiochemically
bacteria,
classify
discuss
breakthroughs
an
emphasis
on
methods
objective
different
uses
beyond
cancer
is
described.
The
combined
strategies
developing
vivo
biotherapeutic
agents
based
these
bacterial
derivatives,
elucidated
how
they
repress
other
also
underlined.
Additionally,
challenges
faced
by
translation
future
development
directions
discussed.
This
review
expected
provide
overall
impression
enlighten
more
researchers.
Language: Английский
Genetically Engineered Bacteria as A Living Bioreactor for Monitoring and Elevating Hypoxia‐Activated Prodrug Tumor Therapy
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 14, 2024
Abstract
Tirapazamine
(TPZ),
an
antitumor
prodrug,
can
be
activated
in
hypoxic
environment.
It
specifically
targets
the
microenvironment
of
tumors
and
produces
toxic
free
radicals.
However,
due
to
tumor
is
not
completely
hypoxic,
TPZ
often
fails
effectively
treat
entire
tissue,
resulting
suboptimal
therapeutic
outcomes.
Herein,
a
low
pathogenic
Escherichia
coli
TOP10
utilized
selectively
colonize
tissues,
disrupt
blood
vessels,
induce
thrombus
formation,
leading
expansion
region
improving
effect
TPZ.
Additionally,
thermosensitive
hydrogel
constructed
by
Pluronic
F‐127
(F127),
which
undergoes
gelation
situ
at
site,
sustained
release
To
monitor
process,
it
genetically
modified
integrating
bioluminescent
system
luxCDABE
(TOP10‐Lux).
The
signal
associated
with
hypoxia
enhancement
beneficial
for
monitoring
bioluminescence
imaging.
In
murine
colon
cancer
model,
TOP10‐Lux
combined
TPZ‐loaded
F127
suppressed
growth,
treatment
process
efficiently
monitored.
Together,
this
work
employs
enhance
efficacy
providing
effective
strategy
bacteria‐based
tumor‐targeted
chemotherapy
monitoring.
Language: Английский