Engineered macrophages: an “Intelligent Repair” cellular machine for heart injury DOI Creative Commons
Zhuo Zhang, Hong Du, Weijie Gao

et al.

Cell Regeneration, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 27, 2024

Abstract Macrophages are crucial in the heart’s development, function, and injury. As part of innate immune system, they act as first line defense during cardiac injury repair. After events such myocardial infarction or myocarditis, numerous macrophages recruited to affected areas heart clear dead cells facilitate tissue This review summarizes roles resident developing cardiovascular diseases. We also describe how macrophage phenotypes dynamically change within disease microenvironment, exhibiting distinct pro-inflammatory anti-inflammatory functions. Recent studies reveal values targeting diseases treatment novel bioengineering technologies engineered a promising therapeutic strategy. Engineered have strong natural tropism infiltration for aiming reduce inflammatory response, inhibit excessive fibrosis, restore function promote regeneration. discuss recent highlighting strategies new approaches macrophages, which can aid recovery.

Language: Английский

Engineered Macrophage Membrane‐Coated S100A9‐siRNA for Ameliorating Myocardial Ischemia‐Reperfusion Injury DOI Creative Commons
Lu H, Junzhuo Wang, Ziwei Chen

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(41)

Published: Sept. 12, 2024

Abstract Despite the widespread adoption of emergency coronary reperfusion therapy, reperfusion‐induced myocardial injury remains a challenging issue in clinical practice. Following reperfusion, S100A8/A9 molecules are considered pivotal initiating and regulating tissue inflammatory damage. Effectively reducing level ischemic holds significant therapeutic value salvaging damaged myocardium. In this study, HA (hemagglutinin)‐ RAGE (receptor for advanced glycation end products)‐ comodified macrophage membrane‐coated siRNA nanoparticles (MMM/RNA NPs) with targeting S100A9 (S100A9‐siRNA) successfully prepared. This nanocarrier system is able to target effectively injured myocardium an environment while evading digestive damage by lysosomes. vivo, migration MMM/RNA NPs lesions confirmed ischemia‐reperfusion (MIRI) mouse model. Intravenous injection significantly reduced levels serum tissues, further decreasing infarction area improving cardiac function. Targeted reduction genetically modified may represent new intervention MIRI.

Language: Английский

Citations

7
Engineered neutrophil membrane-camouflaged nanocomplexes for targeted siRNA delivery against myocardial ischemia reperfusion injury DOI Creative Commons

Yaohui Jiang,

Rong-Yan Jiang,

Zequn Xia

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 22, 2025

Small interfering RNA (siRNA) therapies hold great potential for treating myocardial ischemia-reperfusion injury (MIRI); while their practical application is limited by the low bioavailability, off-target effects, and poor therapeutic efficacy. Here, we present an innovative engineered neutrophil membrane-camouflaged nanocomplex targeted siRNA delivery effective MIRI therapy. A nanoparticle (NP)-based system, namely MNM/siRNA NPs, camouflaged with membranes modified hemagglutinin (HA) integrins. Our comprehensive in vitro studies show that NPs effectively facilitate endosomal escape through HA, achieve excellent targeting via integrins, significantly reduce integrin α9 expression. Furthermore, mice, identify as a target therapy demonstrate decrease infarction area improve cardiac function reducing recruitment, extracellular trap (NET) microthrombus formation. These findings highlight promising platform, offering treatment strategy MIRI.

Language: Английский

Citations

0
S100A8/A9 high-expression macrophages mediate renal tubular epithelial cell damage in acute kidney injury following acute type A aortic dissection surgery DOI Creative Commons

Xiujuan Cai,

Xin Li, Jian Shi

et al.

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: April 9, 2025

Acute kidney injury (AKI) is a major complication after acute type A aortic dissection (ATAAD), with an incidence rate of 20-66.7%. Many patients AKI ATAAD surgery show no clear signs ischemia-reperfusion injury. In our previous study, S100A8 and S100A9 were identified as predictive biomarkers surgery. These proteins are primarily expressed in neutrophils macrophages, where they contribute to cell damage immune activation. However, the roles S100A8/A9 ATAAD-associated remain unclear. this transcriptomics sequence was applied identify differentially genes renal tubular epithelial cells (TCMK-1), stimulated by culture supernatant overexpressed downregulated RAW264.7 cells. Single-cell sequencing data used clusters high expression. Cross-analysis between RNA datasets investigate common pathways enrichment both vitro vivo models. Molecular biology experiments explore downstream signaling S100A8/S100A9. We found that S100A8/S100A9 expression levels increased co-localized macrophages kidneys mice. Marker M1-type like Nos2 Il1b, upregulated while opposite observed group. transcription results, TCMK-1 from can activate TNF PPAR pathway respectively. revealed signaling, IL-17 other inflammatory enriched S100A8/A9-related impairment datasets. Finally, recombinant protein activated findings suggested promising for ATAAD. localized promoted transformation into M1 phenotype. through secretion direct interaction cells, highlighting critical role

Language: Английский

Citations

0
Immune in myocardial ischemia/reperfusion injury: potential mechanisms and therapeutic strategies DOI Creative Commons

Xiaoyu Xuan,

Jilin Fan, Jingyi Zhang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 8, 2025

Myocardial infarction (MI), which is characterized by high morbidity and mortality, a serious threat to human life health, timely reperfusion therapy save ischemic myocardium currently the most effective intervention. Although effectively restores coronary blood flow maximally limits infarct size, it triggers additional cell death tissue damage, known as myocardial ischemia/reperfusion injury (MIRI). Multiple immune cells are present in area, executing specific functions engaging crosstalk during diverse stages, constituting complex microenvironment involved repair regeneration after MIRI. Immunotherapy brings new hope for treating heart disease modulating microenvironment. In this paper, we explore regulatory roles of various MIRI close relationship between different deaths addition, current status research on targeting system intervene MIRI, with expectation providing basis achieving clinical translation.

Language: Английский

Citations

0
Macrophages after myocardial infarction: Mechanisms for repairing and potential as therapeutic approaches DOI
You Yang,

Wu Ai,

Anli Deng

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 143, P. 113562 - 113562

Published: Nov. 12, 2024

Language: Английский

Citations

2
Recent advances in biomimetic nanodelivery systems for the treatment of myocardial ischemia reperfusion injury DOI
Xiaojun Bi, Ze Wang,

Jingteng He

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 247, P. 114414 - 114414

Published: Nov. 28, 2024

Language: Английский

Citations

1
Engineered macrophages: an “Intelligent Repair” cellular machine for heart injury DOI Creative Commons
Zhuo Zhang, Hong Du, Weijie Gao

et al.

Cell Regeneration, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 27, 2024

Abstract Macrophages are crucial in the heart’s development, function, and injury. As part of innate immune system, they act as first line defense during cardiac injury repair. After events such myocardial infarction or myocarditis, numerous macrophages recruited to affected areas heart clear dead cells facilitate tissue This review summarizes roles resident developing cardiovascular diseases. We also describe how macrophage phenotypes dynamically change within disease microenvironment, exhibiting distinct pro-inflammatory anti-inflammatory functions. Recent studies reveal values targeting diseases treatment novel bioengineering technologies engineered a promising therapeutic strategy. Engineered have strong natural tropism infiltration for aiming reduce inflammatory response, inhibit excessive fibrosis, restore function promote regeneration. discuss recent highlighting strategies new approaches macrophages, which can aid recovery.

Language: Английский

Citations

0