High-throughput bioprinting of spheroids for scalable tissue fabrication

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 21, 2024

Tissue biofabrication mimicking organ-specific architecture and function requires physiologically-relevant cell densities. Bioprinting using spheroids can achieve this, but is limited due to the lack of practical, scalable techniques. This study presents HITS-Bio (High-throughput Integrated Fabrication System for Bioprinting), a multiarray bioprinting technique rapidly positioning multiple simultaneously digitally-controlled nozzle array (DCNA). achieves an unprecedented speed, ten times faster compared existing techniques while maintaining high viability ( > 90%). The utility was exemplified in applications, including intraoperative with microRNA transfected human adipose-derived stem calvarial bone regeneration ~ 30 mm3) rat model achieving near-complete defect closure (bone coverage area 91% 3 weeks ~96% 6 weeks). Additionally, successful fabrication cartilage constructs (1 cm3) containing ~600 chondrogenic highlights its high-throughput efficiency (under 40 min per construct) potential repairing volumetric defects. HITS-Bio, platform, assembles mimic native tissue architecture. Its application shows repair rats cartilage.

Language: Английский

High-Throughput Bioprinting of Spheroids for Scalable Tissue Fabrication

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 2, 2024

Tissue biofabrication that replicates an organ-specific architecture and function requires physiologically-relevant cell densities. Bioprinting using spheroids has the potential to create constructs with native densities, but its application is limited due lack of practical, scalable techniques. This study presents HITS-Bio (High-throughput Integrated Fabrication System for Bioprinting), a novel multiarray spheroid bioprinting technology enabling tissue fabrication by rapidly positioning number simultaneously digitally-controlled nozzle array (DCNA) platform. achieves unprecedented speed, order magnitude faster compared existing techniques while maintaining high viability (>90%). The platform's ability pattern multiple enhances rates proportionally size DCNA used. utility was exemplified in applications, including intraoperative microRNA transfected calvarial bone regeneration (∼30 mm

Language: Английский

3D Printed Organisms Enabled by Aspiration‐Assisted Adaptive Strategies

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 21, 2024

Abstract Devising an approach to deterministically position organisms can impact various fields such as bioimaging, cybernetics, cryopreservation, and organism‐integrated devices. This requires continuously assessing the locations of randomly distributed collect transfer them target spaces without harm. Here, aspiration‐assisted adaptive printing system is developed that tracks, harvests, relocates living moving on via a pick‐and‐place mechanism adapts updated visual spatial information about spaces. These strategies successfully positioned single static organism, multiple in droplets, organism Their capabilities are exemplified by vitrification‐ready cryoprotectant sorting live from dead ones, positioning curved surfaces, organizing organism‐powered displays, integrating with materials devices customizable shapes. ultimately lead autonomous biomanufacturing methods evaluate assemble for variety multi‐organism‐based applications.

Language: Английский