Rational Design of a Red-Emitting AIE-ESIPT Probe for Real-Time Visualization of Lipid Droplet Dynamics in Metabolic Stress and Ferroptosis DOI

Haitao Song,

Zhuangzhuang Yang, Yile Zhao

et al.

Dyes and Pigments, Journal Year: 2025, Volume and Issue: unknown, P. 112823 - 112823

Published: April 1, 2025

Language: Английский

General Post‐Regulation Strategy of AIEgens’ Photophysical Properties for Intravital Two‐Photon Fluorescence Imaging DOI Creative Commons
Liyun Lin, Jiaxin Liu, Zhengyuan Pan

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(38)

Published: Aug. 9, 2024

Fluorogens with aggregation-induced emission (AIEgens) are promising agents for two-photon fluorescence (TPF) imaging. However, AIEgens' photophysical properties fixed and unoptimizable once synthesized. Therefore, it is urgent meaningful to explore an efficient post-regulation strategy optimize properties. Herein, a general reported. By simply tuning the ratio of inert AIEgens within binary nanoparticles (BNPs), quantum yield absorption cross-section functional enhanced by 8.7 5.4 times respectively, which not achievable conventional strategies, notorious phototoxicity almost eliminated. The experimental results, theoretical simulation, mechanism analysis demonstrated its feasibility generality. BNPs enabled deep cerebrovascular network imaging ≈1.10 mm depth metastatic cancer cell detection single-cell resolution. Furthermore, TPF quality improved self-supervised denoising algorithm. proposed molecular opened new avenue efficiently boost consequently quality.

Language: Английский

Citations

4
Fast In Vivo Two‐Photon Fluorescence Imaging via Lateral and Axial Resolution Restoration With Self‐Supervised Learning DOI Open Access
Zhengyuan Pan, Man Lei, Hongen Liao

et al.

Journal of Biophotonics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

ABSTRACT Two‐photon fluorescence (TPF) imaging opens a new avenue to achieve high resolution at extended penetration depths. However, it is difficult for conventional TPF systems simultaneously and speed. In this work, we develop an innovative deep learning framework of Lateral Axial Resolution Restoration (LARR) break the contradiction between LARR employs self‐supervised training scheme computationally restore sparsely sampled images isotropic by 4‐fold axial 16‐fold lateral enhancement. The simulation studies experimental results demonstrate excellent performance preserve fine structural features with improved signal‐to‐noise ratio structure similarity index. Moreover, system able 60‐fold speed comparable as compared system. outstanding makes potential tool fast resolution.

Language: Английский

Citations

0
Imaging technology in tracking the intravital fate of transplanted stem cells DOI Creative Commons

Liming Shen,

Chengze Li, Yulian Li

et al.

Pharmacological Research, Journal Year: 2025, Volume and Issue: unknown, P. 107752 - 107752

Published: April 1, 2025

Language: Английский

Citations

0
Rational Design of a Red-Emitting AIE-ESIPT Probe for Real-Time Visualization of Lipid Droplet Dynamics in Metabolic Stress and Ferroptosis DOI

Haitao Song,

Zhuangzhuang Yang, Yile Zhao

et al.

Dyes and Pigments, Journal Year: 2025, Volume and Issue: unknown, P. 112823 - 112823

Published: April 1, 2025

Language: Английский

Citations

0