A Machine Vision Perspective on Droplet‐Based Microfluidics

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Abstract Microfluidic droplets, with their unique properties and broad applications, are essential in chemical, biological, materials synthesis research. Despite the flourishing studies on artificial intelligence‐accelerated microfluidics, most research efforts have focused upstream design phase of microfluidic systems. Generating user‐desired droplets still remains laborious, inefficient, time‐consuming. To address long‐standing challenges associated accurate efficient identification, sorting, analysis morphology generation rate single double emulsion a novel machine vision approach utilizing deformable detection transformer (DETR) algorithm is proposed. This method enables rapid precise (detection relative error < 4% precision > 94%) across various scales scenarios, including real‐world simulated environments. identification (MDIA), web‐based tool powered by Deformable DETR, which supports transfer learning to enhance accuracy specific user scenarios developed. MDIA characterizes diameter, number, frequency, other parameters. As more training data added users, MDIA's capability universality expand, contributing comprehensive database for droplet microfluidics. The work highlights potential intelligence advancing regulation, fabrication, label‐free analysis, accelerating biochemical sciences engineering.

Language: Английский

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Anisotropic Microcarriers: Fabrication Strategies and Biomedical Applications

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Anisotropic microcarriers (AMs) have attracted increasing attention. Although significant efforts been made to explore AMs with various morphologies, their full potential is yet be realized, as most studies primarily focused on materials or fabrication methods. A thorough analysis of the interactional and interdependent relationships between these factors required, along proposed countermeasures tailored for researchers from backgrounds. These include specific strategies morphologies guidelines selecting suitable AM certain biomedical applications. In this review, a comprehensive summary AMs, ranging methods applications, based past two decades research, provided. The investigated using different corresponding By systematically examining morphology-dependent effects, better utilization diverse can achieved clear breakthroughs in field are established. Additionally, challenges identified, new frontiers opened, promising exciting opportunities provided fabricating functional broad implications across fields that must addressed biomaterials biotechnology.

Language: Английский

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Bioinspired spatially ordered multicellular lobules for liver regeneration

Research, Journal Year: 2025, Volume and Issue: 8

Published: Jan. 1, 2025

Cell therapy is a promising strategy for acute liver failure (ALF), while its therapeutic efficacy often limited by cell loss and poor arrangement. Here, inspired microunits, we propose novel spatially ordered multicellular lobules the ALF treatment using microfluidic continuous spinning technology. The microfluidics with multiple microchannels was constructed assembling parallel capillaries. Sodium alginate (Alg) solution encapsulating human umbilical vein endothelial cells (HUVECs), hepatocytes, mesenchymal stem (MSCs) are introduced into middle channel 6 outer channels of microfluidics, respectively. Simultaneously, Ca 2+ -loaded solutions pumped through innermost outermost channels, forming hollow microfiber hepatocytes MSCs alternately surrounding HUVECs. These microfibers could highly resemble cord-like structure lobules, bringing about outstanding liver-like functions. We have demonstrated that in rats, our biomimetic can effectively suppress excessive inflammatory responses, decrease necrosis, promote regenerative pathways, leading to satisfied efficacy. findings underscore potential treating related diseases improving traditional clinical methods.

Language: Английский

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Microfluidic Construction of Heterogeneous Micro–Nano-Structured Materials for Biomedical Applications

ACS Materials Letters, Journal Year: 2025, Volume and Issue: unknown, P. 1462 - 1487

Published: March 20, 2025

Language: Английский

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Microfluidic 3D printing hydrogels based on fish liver decellularized extracellular matrix for liver regeneration

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(4)

Published: Dec. 1, 2024

Abstract Liver tissue engineering offers potential in liver transplantation, while the development of hydrogels for scalable scaffolds incorporating natural components and effective functionalities is ongoing. Here, we propose a novel microfluidic 3D printing hydrogel derived from decellularized fish extracellular matrix regeneration. By decellularizing combining it with gelatin methacryloyl, scaffold retains essential endogenous growth factors such as collagen glycosaminoglycans. Additionally, microfluidic‐assisted technology enables precise modulation composition architecture to fulfill clinical requirements. Benefiting source materials, exhibit excellent biocompatibility cellular proliferation capacity induced pluripotent stem cell‐derived hepatocytes (iPSC‐heps). Furthermore, macroscopic biomechanical environment foster optimal functional expression iPSC‐heps. Importantly, post‐transplantation, significantly enhance survival rates function mice acute failure, promoting regeneration repair. These findings suggest that printed represent promising candidates transplantation recovery.

Language: Английский

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