FoxO1 mediates odontoblast differentiation of hDPSCs via B cell‐derived ANGPTL1 in dental caries: A laboratory investigation

International Endodontic Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Abstract Aim Clinical and in vitro evidence indicates that chronic inflammatory responses initiated by dental caries can persist the pulp even after treatment, necessitating formation of reparative dentin to restore tissue homeostasis health. Human stem cells (hDPSCs) serve as crucial precursors this process. This study explores role B their secreted factor, Angiopoietin Like 1 (ANGPTL1), promoting hDPSCs differentiation into odontoblasts under carious conditions, with a particular focus on activation Forkhead box O1 (FoxO1). Methodology Single‐cell RNA sequencing (scRNA‐Seq) data from GEO database were analysed explore cellular interactions molecular mechanisms pulp. Immunofluorescence staining was used investigate expression patterns or hydroxyapatite/tricalcium phosphate (HA/TCP) scaffolds. The levels ANGPTL1 quantified using enzyme‐linked immunosorbent assay (ELISA). Odontoblast capacity assessed alkaline phosphatase activity, alizarin red S staining, western blotting analysis. overexpressed knocked down FoxO1 lentiviruses. regulatory interaction between DSPP promoter evaluated through dual‐luciferase reporter chromatin immunoprecipitation assay. Statistical analyses conducted Student's t ‐test one‐way analysis variance ( anova ) p ‐value <.05 considered statistically significant. Results scRNA‐Seq indicated significant increase Functional confirmed activated hDPSCs, enhancing odontoblast‐like cells. Blocking signalling specific antibody reduced expression, indicating link FoxO1. Overexpression promoted facilitated mineralized matrix formation. Mechanistic studies revealed directly binds promoter, thereby inducing its expression. Conclusions Our reveals novel mechanism which environment promotes odontoblast upregulating finding highlights potential therapeutic target for repair regeneration.

Language: Английский

3D visualization of neurovascular networks in pulp-exposed rat molars using tissue clearing techniques

Odontology, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Language: Английский