Construction of an Efficient Engineered Strain for Chaetoglobosin A Bioresource Production from Potato Starch Industrial Waste

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 842 - 842

Published: Feb. 28, 2025

Chaetoglobosin A (CheA), a typical structure of the cytochalasin family, exhibits outstanding efficacy against variety tumor cells and plant pathogens. However, its low yield high production cost are major obstacles limiting wide application. In order to increase CheA yield, an engineered strain was established by overexpressing CgMfs, gene encoding MFS family's efflux pump, on chassis lacking CgXpp1, which have been shown act as negative regulator biosynthesis. As expected, significantly boosted from 63.19 265.93 mg/L after incubation in PDA medium for 10 d, whereas remarkably enhanced 2.93-fold compared with wild type, following d cultivation utilizing potato starch industrial waste. The addition metal ions had positive effect production, Cu2+ being most effective improving 176.92 mg/L. optimal fermentation conditions were determined response surface optimization, under conditions, could stably produce 197.58 This study provided reducing costs while increasing well new strategies insights target compound.

Language: Английский

Genome Mining of Epicoccum dendrobii Reveals Diverse Antimicrobial Natural Products

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Epicoccum dendrobii, a biocontrol fungus, produces bioactive metabolites with potential agricultural applications. Targeted genome mining revealed 13 compounds, including three novel polyketides (epiketides A-C, 1-3), new natural product (epiketide D, 4), diketopiperazine (boydine E, 5), and eight known analogues 6-13. Structures were elucidated via NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), computational methods. Compounds 1, 2 4 exhibited moderate antibacterial activity against Bacillus licheniformis (minimum inhibitory concentrations (MICs): 5.5-37.6 μM) Pseudomonas aeruginosa (MICs: 11.1-20.0 μM). A protoplast transformation system was developed to identify the biosynthetic gene cluster eda responsible for diketopiperazines 5-10. Antifungal assays that wild-type E. dendrobii inhibited plant pathogen Botrytis cinerea by 56.03%, while ΔedaP mutant (lacking diketopiperazines) showed reduced efficacy, implying role of in antifungal activity. This study underscores dendrobii's as agent provides insights into its

Language: Английский