An “all-in-one” therapeutic platform for programmed antibiosis, immunoregulation and neuroangiogenesis to accelerate diabetic wound healing

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123293 - 123293

Published: March 1, 2025

Language: Английский

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In vitro study on the promotion of osteogenic differentiation by mitochondrial-derived vesicles through activation of inflammation and reprogramming of metabolic pathways

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 17, 2025

The secretion of mitochondrial-derived vesicles (MDVs) has been found to increase during osteogenic differentiation, but their role in intercellular communication and promotion remains unclear. In this study, we extracted translocase outer mitochondrial membrane 20 (Tomm20) + MDVs from bone marrow stromal cells (BMSCs) at different culture days using differential centrifugation immunoprecipitation, then co-cultured them with BMSCs assess immune response metabolic levels. results showed that differentiation enhances MDVs' DNA (mtDNA) content. promoting ability, osteogenic-induced (MDV-OMs, especially MDV-OM14 MDV-OM21) significantly enhance mineralization OD values 1.37-fold 1.32-fold higher than those MDV-OM7 (p < 0.05) after 21 days, respectively. However, these containing mtDNA activate responses by upregulating cGas, Sting, Caspase-9, Il-6, Tnf-a mRNA levels, inducing cell apoptosis oxidative stress. addition, components also have regulatory functions. Metabolic level detection revealed MDV-OMs downregulate lactate, promote tricarboxylic acid cycle (TCA) enzyme expression, potential. Among MDVs, MDV-OM7, induced for 7 shows function without strong response, possibly related reprogramming. This study highlights the potential regeneration, cGAS-STING activation, enhancement, are expected be used treatment diseases such as tissue damage.

Language: Английский

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Extracellular Vesicles and Their Role in Skin Inflammatory Diseases: From Pathogenesis to Therapy

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3827 - 3827

Published: April 18, 2025

Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, are released into the extracellular space by almost all known cell types. They facilitate communication between cells transferring bioactive molecules, which impact both physiological processes development of diseases. EVs play a crucial role in pathogenesis various diseases participating multiple pathological processes. contribute to disease progression triggering cytokine release, modulating immune activity, inducing inflammatory responses. Beyond their implications, also offer significant therapeutic potential. Both natural engineered show great potential fields targeted therapy, drug delivery, modulation dermatological applications. The EV-based treatments is showing promise advancing patient outcomes, particularly chronic immune-mediated skin conditions. This review comprehensively examined biogenesis, classification, functional roles EVs, advanced methods for isolation characterization. Furthermore, we summarized recent studies highlighting involvement four major diseases: psoriasis, atopic dermatitis, systemic lupus erythematosus, wound healing.

Language: Английский

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Impact of Rehabilitation Management on Postoperative Complications in Diabetic Foot Debridement

Current Problems in Surgery, Journal Year: 2025, Volume and Issue: unknown, P. 101777 - 101777

Published: April 1, 2025

Language: Английский

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Ahr Deficiency Exacerbates Inflammation in Diabetic Wounds Via Impaired Mitophagy and Cgas-Sting-Nlrp3 Activation: Therapeutic Potential of Hydrogels Loaded with Ficz

Published: Jan. 1, 2025

Language: Английский

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