In Situ Lighting-Up Near-Infrared Metabolic Probes for Super-Resolution Imaging of Glycogen

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

High-fidelity tracking of glycogen dynamics in living biosystems is critical for exploring the biological role metabolism diseases. However, situ information on state mainly relies a glucose uptake fluorescence probe 2-NBDG, which has proven to be extremely limited owing "always-on" fluorescence, short emission wavelength, and low signal-to-noise (S/N) ratio. Herein, we first time demonstrate metabolic-activated off-on through covalently attaching molecular rotor with hydrophilic at C-2 position guarantee good miscibility complete fluorescence-off before metabolic incorporation into particles. The Glycogen-Red achieves negligible background (1/30 times than 2-NBDG) an ultrahigh S/N ratio (61-fold lighting-up near-infrared (NIR) biosynthesis. Notably, our unique characteristic bypassing washing steps, offering powerful toolbox real-time biosynthesis super-resolution mapping structures cells.

Language: Английский

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Rational development of Nile Red derivatives with significantly improved specificity and photostability for advanced fluorescence imaging of lipid droplets

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 282, P. 117494 - 117494

Published: April 17, 2025

Language: Английский

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Dibutylated Si-fluorescein: enhanced hydrophobicity for fluorogenic labeling in vivo.

Dyes and Pigments, Journal Year: 2025, Volume and Issue: unknown, P. 112841 - 112841

Published: April 1, 2025

Language: Английский

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Trends in small-molecule probes for lipid droplets

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118287 - 118287

Published: May 1, 2025

Language: Английский

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