Bioinspired spatially ordered multicellular lobules for liver regeneration DOI Creative Commons

Jinglin Wang,

Danqing Huang, Haozhen Ren

et al.

Research, Journal Year: 2025, Volume and Issue: 8

Published: Jan. 1, 2025

Cell therapy is a promising strategy for acute liver failure (ALF), while its therapeutic efficacy often limited by cell loss and poor arrangement. Here, inspired microunits, we propose novel spatially ordered multicellular lobules the ALF treatment using microfluidic continuous spinning technology. The microfluidics with multiple microchannels was constructed assembling parallel capillaries. Sodium alginate (Alg) solution encapsulating human umbilical vein endothelial cells (HUVECs), hepatocytes, mesenchymal stem (MSCs) are introduced into middle channel 6 outer channels of microfluidics, respectively. Simultaneously, Ca 2+ -loaded solutions pumped through innermost outermost channels, forming hollow microfiber hepatocytes MSCs alternately surrounding HUVECs. These microfibers could highly resemble cord-like structure lobules, bringing about outstanding liver-like functions. We have demonstrated that in rats, our biomimetic can effectively suppress excessive inflammatory responses, decrease necrosis, promote regenerative pathways, leading to satisfied efficacy. findings underscore potential treating related diseases improving traditional clinical methods.

Language: Английский

Bioinspired spatially ordered multicellular lobules for liver regeneration DOI Creative Commons

Jinglin Wang,

Danqing Huang, Haozhen Ren

et al.

Research, Journal Year: 2025, Volume and Issue: 8

Published: Jan. 1, 2025

Cell therapy is a promising strategy for acute liver failure (ALF), while its therapeutic efficacy often limited by cell loss and poor arrangement. Here, inspired microunits, we propose novel spatially ordered multicellular lobules the ALF treatment using microfluidic continuous spinning technology. The microfluidics with multiple microchannels was constructed assembling parallel capillaries. Sodium alginate (Alg) solution encapsulating human umbilical vein endothelial cells (HUVECs), hepatocytes, mesenchymal stem (MSCs) are introduced into middle channel 6 outer channels of microfluidics, respectively. Simultaneously, Ca 2+ -loaded solutions pumped through innermost outermost channels, forming hollow microfiber hepatocytes MSCs alternately surrounding HUVECs. These microfibers could highly resemble cord-like structure lobules, bringing about outstanding liver-like functions. We have demonstrated that in rats, our biomimetic can effectively suppress excessive inflammatory responses, decrease necrosis, promote regenerative pathways, leading to satisfied efficacy. findings underscore potential treating related diseases improving traditional clinical methods.

Language: Английский

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