Antibacterial Peptides-Produced Cell Hydrogel Eliminates Intracellular Pathogens and Remodels Immune Microenvironment for Osteomyelitis Therapy DOI

Yu Chen,

Qi‐Wen Chen,

Fang-Sheng Fu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Featuring bacterial invasion and colonization within cells, chronic infection-induced immune suppression, inflammatory cell infiltration, osteomyelitis is currently an intractable recurrent bone disease. In this study, injectable hydrogel that gels in situ loaded with engineered antimicrobial cells (LL37-MSC@OCAHM) developed. This anti-inflammatory not only maintains activity the environment but also releases magnesium ions (Mg2+) to promote differentiation of MSCs into bone-forming contributing mass formation, enhancing repair, accelerating healing. The continuously produce peptides LL37, which effectively kill both extracellular intracellular bacteria at site. Additionally, modulates response by shifting from pro-inflammatory anti-inflammatory, reducing infiltration myeloid-derived suppressor (MDSCs). We demonstrated its ability activate responses generate memory, thereby preventing recurrence secondary infections. study introduces cell-based approach combines active effect, modulation, for eliminating infections recurrence.

Language: Английский

Antibacterial Peptides-Produced Cell Hydrogel Eliminates Intracellular Pathogens and Remodels Immune Microenvironment for Osteomyelitis Therapy DOI

Yu Chen,

Qi‐Wen Chen,

Fang-Sheng Fu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Featuring bacterial invasion and colonization within cells, chronic infection-induced immune suppression, inflammatory cell infiltration, osteomyelitis is currently an intractable recurrent bone disease. In this study, injectable hydrogel that gels in situ loaded with engineered antimicrobial cells (LL37-MSC@OCAHM) developed. This anti-inflammatory not only maintains activity the environment but also releases magnesium ions (Mg2+) to promote differentiation of MSCs into bone-forming contributing mass formation, enhancing repair, accelerating healing. The continuously produce peptides LL37, which effectively kill both extracellular intracellular bacteria at site. Additionally, modulates response by shifting from pro-inflammatory anti-inflammatory, reducing infiltration myeloid-derived suppressor (MDSCs). We demonstrated its ability activate responses generate memory, thereby preventing recurrence secondary infections. study introduces cell-based approach combines active effect, modulation, for eliminating infections recurrence.

Language: Английский

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