Programmable Manually Powered Microfluidics for Rapid Point-of-Care Diagnosis of Urinary Tract Infection DOI

Longyu Yi,

Yuqi Liu, Chao Wan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Point-of-care testing (POCT) for urinary tract infection (UTI) holds significant importance in the field of disease prevention and control, as well advancement personalized precision medicine. However, conventional methods detecting UTIs continue to face challenges such time-consuming labor-intensive detection processes, reliance on specialized equipment personnel rendering them unsuitable point-of-care applications, especially resource-limited areas. Here, we propose a novel flexible programmable manually powered microfluidic (FPM) rapid diagnosis UTIs. For first time, proposed FPMs was achieved through combined strategy laser printing, cutting, laminating, with entire process completed under 15 min at cost less than $0.5, which effectively circumvent traditionally soft lithography techniques. By incorporating modular structure-based design concept, successfully developed various types portable functionalities including parallel pumping, simultaneous releasing, quantitative dispensing, sequential cyclic motion multiple liquids concentration gradient generating. As proof-of-concept demonstration, initially employed high-throughput dispensing analyze six biochemical markers within 1 min, presenting potential applicability future at-home testing. We then integrated generator spatial confinement signal enhancement enable phenotypic antimicrobial susceptibility (AST) three 5 h, while achieving clinical diagnostic accuracy rates up 95.56%. Therefore, our eliminate need external pumps or actuators could serve an affordable hand-held POCT tool UTI diagnosis. Moreover, resource-poor areas, they have utility robust devices addressing diverse needs.

Language: Английский

Fully Integrated Centrifugal Microfluidics for Rapid Exosome Isolation, Glycan Analysis, and Point-of-Care Diagnosis DOI
Xudong Zhao, Xiang Liu, Tucan Chen

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Exosomes present in the circulatory system demonstrate considerable promise for diagnosis and treatment of diseases. Nevertheless, complex nature blood samples prevalence highly abundant proteins pose a significant obstacle to prompt effective isolation functional evaluation exosomes from blood. Here, we fully integrated lab-on-a-disc equipped with two nanofilters, also termed iExoDisc, which facilitates automated 400 μL within 45 min. By integrating plasma separation module, protein removal nanopore membrane-based total resulting exhibited significantly increased purity (∼3-6-fold) compared conventional ultracentrifugation polymer precipitation. Additionally, then successfully performed nontargeted targeted glycan profiling on derived clinical triple-negative breast cancer (TNBC) patients using MALDI-TOF-MS lectin microarray containing 56 kinds lectins. The findings both methodologies indicated that galactosylation sialylation exhibit potential as diagnostic indicators TNBC. Finally, by utilizing exosome-specific glycosylated CD63 proof-of-concept, realized integration point-of-care on-chip exosome situ detection 2 h. Thus, iExoDisc provides approach early detection, liquid biopsy, diagnosis.

Language: Английский

Citations

1
Programmable Manually Powered Microfluidics for Rapid Point-of-Care Diagnosis of Urinary Tract Infection DOI

Longyu Yi,

Yuqi Liu, Chao Wan

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Point-of-care testing (POCT) for urinary tract infection (UTI) holds significant importance in the field of disease prevention and control, as well advancement personalized precision medicine. However, conventional methods detecting UTIs continue to face challenges such time-consuming labor-intensive detection processes, reliance on specialized equipment personnel rendering them unsuitable point-of-care applications, especially resource-limited areas. Here, we propose a novel flexible programmable manually powered microfluidic (FPM) rapid diagnosis UTIs. For first time, proposed FPMs was achieved through combined strategy laser printing, cutting, laminating, with entire process completed under 15 min at cost less than $0.5, which effectively circumvent traditionally soft lithography techniques. By incorporating modular structure-based design concept, successfully developed various types portable functionalities including parallel pumping, simultaneous releasing, quantitative dispensing, sequential cyclic motion multiple liquids concentration gradient generating. As proof-of-concept demonstration, initially employed high-throughput dispensing analyze six biochemical markers within 1 min, presenting potential applicability future at-home testing. We then integrated generator spatial confinement signal enhancement enable phenotypic antimicrobial susceptibility (AST) three 5 h, while achieving clinical diagnostic accuracy rates up 95.56%. Therefore, our eliminate need external pumps or actuators could serve an affordable hand-held POCT tool UTI diagnosis. Moreover, resource-poor areas, they have utility robust devices addressing diverse needs.

Language: Английский

Citations

0