A Smart MMP‐9‐responsive Hydrogel Releasing M2 Macrophage‐derived Exosomes for Diabetic Wound Healing

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 16, 2025

Chronic diabetic wounds are characterized by prolonged inflammation and excessive accumulation of M1 macrophages, which impede the healing process. Therefore, resolving promptly transitioning to proliferative phase critical steps for effective wound healing. Exosomes have emerged as a promising therapeutic strategy. In this study, smart hydrogel capable responding pathological cues in inflammatory microenvironment promote transition from proliferation delivering M2 macrophage-derived exosomes (M2-Exos) is developed. The synthesized through cross-linking oxidized dextran, matrix metalloproteinase (MMP)-9-sensitive peptide, carboxymethyl chitosan containing M2-Exos. response elevated MMP-9 concentrations microenvironment, demonstrates diagnostic logic, adjusting release kinetics M2-Exos accordingly. on-demand facilitated macrophage polarization phenotype, thereby promoting accelerating transcriptomic analysis further reveals that MMP-9-responsive with delivery exerts anti-inflammatory regenerative effects downregulating inflammation-related pathways. This study introduces an innovative, microenvironment-responsive exosome system enables precise control agent release, offering personalized approach treatment chronic wounds.

Language: Английский

2A-biohydrogels accelerate diabetic wound healing by promoting M2 macrophage polarization and functionalized mitochondrial transfer to endothelial cells

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 514, P. 163130 - 163130

Published: April 28, 2025

Language: Английский

Innovative Hydrogel Design: Tailoring Immunomodulation for Optimal Chronic Wound Recovery

Advanced Science, Journal Year: 2024, Volume and Issue: 12(2)

Published: Nov. 22, 2024

Abstract Despite significant progress in tissue engineering, the full regeneration of chronic wounds persists as a major challenge, with immune response to damage being key determinant healing process's quality and duration. Post‐injury, crucial aspect is transition macrophages from pro‐inflammatory state an anti‐inflammatory. Thus, this alteration macrophage polarization presents enticing avenue within realm regenerative medicine. Recent advancements have entailed integration myriad cellular molecular signals into hydrogel‐based constructs, enabling fine‐tuning cell activities during different phases. This discussion explores modern insights roles skin regeneration, underscoring role modulation amplifying overall efficacy wounds. Moreover, comprehensive review presented on latest sophisticated technologies employed design immunomodulatory hydrogels regulate polarization. Furthermore, deliberate deliver targeted stimulation through manipulation chemistry also emphasized. overview provided regarding influence hydrogel properties traits process. Conclusively, accent forthcoming pathways directed toward modulating responses milieu healing.

Language: Английский