Label‐Free Microscale Technologies for Isolation of Heterogeneous Circulating Tumor Cells DOI Creative Commons
Gürhan Özkayar,

Esma Derin,

Georg R. Pesch

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

The dissemination of primary solid tumor cells to distant organs, termed metastasis, is a major cause cancer‐related deaths. Circulating (CTCs), which can exist as individual or multicellular clusters, travel through the bloodstream. Their isolation from liquid biopsy samples increasingly recognized valuable tool for diagnosis, prognosis, and treatment guidance cancer patients. Current methods typically rely on biomarkers like epithelial cell adhesion molecule (EpCAM) utilize technologies such magnetic beads microfluidic chips. However, these face limitations due heterogeneity. Furthermore, that transfer into CTCs often undergo epithelial‐to‐mesenchymal transition, gaining invasive characteristics while losing markers. As result, are difficult detect using EpCAM‐based methods. Label‐free microscale tackle biomarker‐based by leveraging distinctive physical properties CTCs, their size, electrical charge, viscoelasticity, deformability contrast them normal blood cells. This review evaluates label‐free methods, including deterministic lateral displacement, microfiltration, acoustophoresis, dielectrophoresis, whether they offer deeper insight heterogeneity dynamics progression treatment. Additionally, it highlights automated platforms high‐throughput CTC analysis.

Language: Английский

Microfluidic Biosensors: Enabling Advanced Disease Detection DOI Creative Commons

S. Wang,

Xiaotian Guan,

Shuqing Sun

et al.

Sensors, Journal Year: 2025, Volume and Issue: 25(6), P. 1936 - 1936

Published: March 20, 2025

Microfluidic biosensors integrate microfluidic and biosensing technologies to achieve the miniaturization, integration, automation of disease diagnosis, show great potential for application in fields cancer liquid biopsy, pathogenic bacteria detection, POCT. This paper reviews recent advances related field laboratory medicine, focusing on their applications above three areas. In facilitate isolation, enrichment, detection tumor markers such as CTCs, ctDNA, miRNA, exosomes, so on, providing support early precise treatment, prognostic assessment. terms can rapid, highly sensitive, specific a variety bacteria, helping prevention control well public health safety. Pertaining realm POCT, bring convenient diseases, tumors, infectious chronic primary care. Future biosensor research will focus enhancing throughput, lowering costs, innovating new recognition elements signal transduction methods, integrating artificial intelligence, broadening include home care, drug discovery, food safety, on.

Language: Английский

Citations

0
Label‐Free Microscale Technologies for Isolation of Heterogeneous Circulating Tumor Cells DOI Creative Commons
Gürhan Özkayar,

Esma Derin,

Georg R. Pesch

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

The dissemination of primary solid tumor cells to distant organs, termed metastasis, is a major cause cancer‐related deaths. Circulating (CTCs), which can exist as individual or multicellular clusters, travel through the bloodstream. Their isolation from liquid biopsy samples increasingly recognized valuable tool for diagnosis, prognosis, and treatment guidance cancer patients. Current methods typically rely on biomarkers like epithelial cell adhesion molecule (EpCAM) utilize technologies such magnetic beads microfluidic chips. However, these face limitations due heterogeneity. Furthermore, that transfer into CTCs often undergo epithelial‐to‐mesenchymal transition, gaining invasive characteristics while losing markers. As result, are difficult detect using EpCAM‐based methods. Label‐free microscale tackle biomarker‐based by leveraging distinctive physical properties CTCs, their size, electrical charge, viscoelasticity, deformability contrast them normal blood cells. This review evaluates label‐free methods, including deterministic lateral displacement, microfiltration, acoustophoresis, dielectrophoresis, whether they offer deeper insight heterogeneity dynamics progression treatment. Additionally, it highlights automated platforms high‐throughput CTC analysis.

Language: Английский

Citations

0