Exosome-derived miR-548ag drives hepatic lipid accumulation via upregulating FASN through inhibition of DNMT3B DOI Creative Commons

Xiaolong Chu,

Yanting Hou,

Chaoling Peng

et al.

Journal of Lipid Research, Journal Year: 2025, Volume and Issue: unknown, P. 100818 - 100818

Published: May 1, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is the leading cause of chronic worldwide. This study investigates role serum miR-548ag in regulating lipid metabolism and its contribution to MASLD obesity. We found that levels were significantly elevated both obese patients, positively correlated with body mass index (BMI), fasting plasma glucose (FPG), triglycerides (TG), total cholesterol (TC), LDL, HDL, aspartate aminotransferase (AST) alanine (ALT) levels. Additionally, expression was higher abdominal adipose tissue individuals compared those normal weight. In vitro studies HepG2 L02 cells, along previous findings, demonstrated promotes fatty acid synthase (FASN) by inhibiting DNA methyltransferase 3B (DNMT3B), thereby enhancing synthesis. confirmed two mouse models: one tail vein injections mimic/inhibitor adeno-associated viruses, another exosomes from normal-weight individuals. Both models showed upregulated FASN through DNMT3B inhibition, increased synthesis larger hepatic droplets, effects reversed inhibition. Taken together, obesity enhances targeting upregulate FASN, contributing development MASLD.

Language: Английский

Exosome-derived miR-548ag drives hepatic lipid accumulation via upregulating FASN through inhibition of DNMT3B DOI Creative Commons

Xiaolong Chu,

Yanting Hou,

Chaoling Peng

et al.

Journal of Lipid Research, Journal Year: 2025, Volume and Issue: unknown, P. 100818 - 100818

Published: May 1, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is the leading cause of chronic worldwide. This study investigates role serum miR-548ag in regulating lipid metabolism and its contribution to MASLD obesity. We found that levels were significantly elevated both obese patients, positively correlated with body mass index (BMI), fasting plasma glucose (FPG), triglycerides (TG), total cholesterol (TC), LDL, HDL, aspartate aminotransferase (AST) alanine (ALT) levels. Additionally, expression was higher abdominal adipose tissue individuals compared those normal weight. In vitro studies HepG2 L02 cells, along previous findings, demonstrated promotes fatty acid synthase (FASN) by inhibiting DNA methyltransferase 3B (DNMT3B), thereby enhancing synthesis. confirmed two mouse models: one tail vein injections mimic/inhibitor adeno-associated viruses, another exosomes from normal-weight individuals. Both models showed upregulated FASN through DNMT3B inhibition, increased synthesis larger hepatic droplets, effects reversed inhibition. Taken together, obesity enhances targeting upregulate FASN, contributing development MASLD.

Language: Английский

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