Excited-State-Altering Ratiometric Fluorescent Probes for the Response of β-Galactosidase in Senescent Cells DOI Creative Commons

Yanan Han,

Lei Dong,

Lu-Lu Sun

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(6), P. 1221 - 1221

Published: March 8, 2025

β-galactosidase (β-Gal) has emerged as a pivotal biomarker for the comprehensive investigation of diseases associated with cellular senescence. The development fluorescent sensor is considerable importance precisely detecting activity and spatial distribution β-Gal. In this study, we developed two excited-state-altering responsive sensors (TF1 TF2) ratiometric detection Two TCF dyes, composed tricyanofuran (TCF) naphthol units, feature electron “pull–push” systems are quenched fluorescence by Upon β-Gal hydrolysis, significant shift in absorption from ca. 475 nm to 630 observed, accompanied emergence signal at 660 nm. enzyme-responsive optical red-shifts attributed excited-state transition intramolecular charge transfer (ICT) state local excited (LE) state, which was confirmed density functional theory (DFT) calculations. Both display exceptional sensitivity selectivity response PBS solution capable tracking within senescent A549 cells. This study introduces framework developing multimodal probes systematically modulating properties, demonstrating their utility senescence studies, diagnostic assay design, therapeutic assessment.

Language: Английский

Excited-State-Altering Ratiometric Fluorescent Probes for the Response of β-Galactosidase in Senescent Cells DOI Creative Commons

Yanan Han,

Lei Dong,

Lu-Lu Sun

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(6), P. 1221 - 1221

Published: March 8, 2025

β-galactosidase (β-Gal) has emerged as a pivotal biomarker for the comprehensive investigation of diseases associated with cellular senescence. The development fluorescent sensor is considerable importance precisely detecting activity and spatial distribution β-Gal. In this study, we developed two excited-state-altering responsive sensors (TF1 TF2) ratiometric detection Two TCF dyes, composed tricyanofuran (TCF) naphthol units, feature electron “pull–push” systems are quenched fluorescence by Upon β-Gal hydrolysis, significant shift in absorption from ca. 475 nm to 630 observed, accompanied emergence signal at 660 nm. enzyme-responsive optical red-shifts attributed excited-state transition intramolecular charge transfer (ICT) state local excited (LE) state, which was confirmed density functional theory (DFT) calculations. Both display exceptional sensitivity selectivity response PBS solution capable tracking within senescent A549 cells. This study introduces framework developing multimodal probes systematically modulating properties, demonstrating their utility senescence studies, diagnostic assay design, therapeutic assessment.

Language: Английский

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