A self‐regulated phototheranostic nanosystem with single wavelength‐triggered energy switching and oxygen supply for multimodal synergistic therapy of bacterial biofilm infections

Aggregate, Journal Year: 2024, Volume and Issue: 5(5)

Published: May 13, 2024

Abstract The exploration of antibiotic‐independent phototherapy strategies for the treatment bacterial biofilm infections has gained significant attention. However, efficient eradication biofilms remains a challenge. Herein, self‐regulated phototheranostic nanosystem with single wavelength‐triggered photothermal therapy (PTT)/photodynamic (PDT) transformation and oxygen supply multimodal synergistic is presented. This approach combines eutectic mixture natural phase‐change materials (PCMs) an aggregation‐induced emission (AIE) agent TPA‐ICN to form colloidally stable nanopartcicles (i.e. AIE@PCM NPs). reversible solid−liquid phase transition PCMs facilitates adaptive regulation aggregation states TPA‐ICN, enabling switch between energy dissipation pathways enhanced PDT in solid or PTT liquid PCMs. Additionally, oxygen‐carrying thermoresponsive nanoparticles are also introduced alleviate hypoxic microenvironment by releasing upon heating NPs PTT. exhibits outstanding therapeutic efficacy against both vitro vivo, antibacterial efficiency 99.99%. study utilizes theranostic nanoplatform PTT/PDT via heat‐triggered release, holding great promise safe infections.

Language: Английский

---

Anthraquinone-Centered Type I Photosensitizer with Aggregation-Induced Emission Characteristics for Tumor-Targeted Two-Photon Photodynamic Therapy

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 6(6), P. 2174 - 2185

Published: May 6, 2024

Exploring advanced photodynamic strategies to surmount the firm reliance on oxygen and limited penetration depth of excitation light is great significance in tumor therapy. Herein, a two-photon excitable type I photosensitizer with aggregation-induced emission characteristics, namely tBuT2AQ, rationally designed by simply equipping an anthraquinone (AQ) acceptor rotor-type electron-donating triphenylamine derivatives. The AQ moiety good electron-withdrawing ability significant spin–orbit coupling effect could facilitate generation triplet excitons triggering multichannel intersystem crossing. These further undergo electron transfer processes enable massive production reactive species because redox cycling behaviors mediated carbonyl groups moiety. Moreover, planar structure beneficial for allows deep into biological tissues. After being formulated nanoparticles acid-responsive charge-reversible polymers, tBuT2AQ can be accumulated at sites efficiently performs highly effective treatment.

Language: Английский

---

A pH‐Responsive, Surface Charge‐Switchable Nanosystem with Enhanced Biofilm Penetration for Synergistic Photodynamic and Antibiotic Therapy of Diabetic Wounds

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

Abstract Chronic wounds, particularly those associated with diabetes, pose a significant clinical challenge due to their tendency develop biofilms that resist conventional antibiotic treatments. To address this issue, novel therapeutic strategy utilizing pH‐responsive nanoparticles loaded aggregation‐induced emission photosensitizers and natural saturated fatty acids (AIE/LA@HMONs−PyB) for effective biofilm penetration disruption is proposed. Under physiological conditions, AIE/LA@HMONs−PyB are negatively charged. Upon accumulation at infected sites, however, the pyridine betaine group on surface of enables rapid protonation charge reversal in acidic microenvironment, thereby enhancing ability penetrate biofilm. light irradiation, these generate reactive oxygen species effectively disrupt structure. This process synergistic action ciprofloxacin lower concentration, achieving an exceptional vitro antibacterial efficiency 99.99% against methicillin‐resistant Staphylococcus aureus ( S. ) biofilms. Furthermore, vivo diabetic wound model, therapy accelerates healing by reducing inflammation, promoting angiogenesis, collagen regeneration. The enhanced significantly improves efficacy combined approach, offering great promise advancing chronic patient outcomes.

Language: Английский

---

A straightforward strategy to modulate ROS generation of AIE photosensitizers for type-I PDT

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 155782 - 155782

Published: Sept. 1, 2024

Language: Английский

---

Copper‐infused zinc porphyrin supramolecular polymersomes for triple synergistic combat against wound bacterial biofilm infections

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Abstract Bacterial biofilm infections (BBI) on wound surfaces and implants pose a significant clinical challenge due to the impermeable nature of biofilms obstacles in tissue repair. Traditional photothermal therapy (PTT) or chemodynamic (CDT), whether used alone combination, often causes damage surrounding normal tissues from high operating temperatures elevated levels reactive oxygen species, leading unsatisfactory anti‐biofilm effects. This study introduces novel copper ions loaded zinc porphyrin polymer vesicle (Cu‐PPS) that employs triple synergistic approach involving PTT, CDT, bacterial cuproptosis‐like death (BCD). Cu‐PPS exhibits exceptional efficiency 51.06%, promoting release under stimulation, enhancing CDT BCD effectiveness, facilitating mature clearance achieves bactericidal rate exceeding 99% an 93.32% vitro also excellent efficacy treating BBI vivo. presents innovative therapeutic strategy for combating infections, addressing challenges encountered management BBI.

Language: Английский

---

pH‐Responsive Protein‐Polycation Nanocarriers for Efficient Eradication of Bacterial Biofilms and Intracellular Bacteria

Macromolecular Rapid Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Bacterial biofilms and intracellular pathogens pose significant challenges in eradication, often leading to persistent infections that are difficult treat. To address this issue, the hydrophobic biofilm dispersant D-tyrosine is encapsulated within protein-polycation nanoparticles, designed using a mannose-terminated cationic polymer concanavalin through electrostatic interactions. Thermodynamic studies reveal free mannosyl groups on nanoparticle surface promote spontaneous binding receptor molecules mimicking those bacterial host cells. Under mildly acidic conditions, nanoparticles reduce size from 550 ≈48 nm 2 h, releasing 76% of D-tyrosine. The combination mannose targeting, particle reduction, controlled release enable eliminate 70%-80% Pseudomonas aeruginosa Staphylococcus aureus biomass at minimum bactericidal concentration (MBC) 2MBC while eradicating 8 log bacteria embedded biofilm. In an infection model RAW 264.7 macrophages, over 95% without inducing increase inflammatory cytokine interleukin-6. These which activate their antimicrobial properties under efficiently penetrate cell barriers via mannose-rich surface, offering promising strategy for treatment infections.

Language: Английский

---

Optogenetic patterning generates multi-strain biofilms with spatially distributed antibiotic resistance

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 1, 2024

Spatial organization of microbes in biofilms enables crucial community function such as division labor. However, quantitative understanding emergent properties remains limited due to a scarcity tools for patterning heterogeneous biofilms. Here we develop synthetic optogenetic toolkit 'Multipattern Biofilm Lithography' rational engineering and orthogonal multi-strain biofilms, inspired by successive adhesion phenotypic differentiation natural We apply this profile the growth dynamics biofilm communities, observe emergence spatially modulated commensal relationships shared antibiotic protection against beta-lactam ampicillin. Supported biophysical modeling, these results yield in-vivo measurements key parameters, e.g., molecular beta-lactamase production per cell length scale zone protection. Our toolbox associated findings provide insights into spatial distributed within with direct implications future research engineering.

Language: Английский

---

Ionic Modification of Three-Component Covalent Organic Frameworks with Antibacterial Function for Efficient and Selective Gold Recovery

ACS Materials Letters, Journal Year: 2024, Volume and Issue: 7(1), P. 220 - 228

Published: Dec. 13, 2024

Developing multifunctional adsorbents with exceptional capture performance and outstanding antibiofouling activity is of great significance for the recovery gold. However, most reported materials only have a single adsorption function, which restricts their practical application gold extraction from complex matrices. Herein, we construct series three-component covalent organic frameworks (COFs) good antibacterial All obtained COFs show high capacities over 1250 mg·g–1 gold, may be attributed to specific surface area, regular pore structure abundant binding sites. In particular, optimized COF-TPTD-DHTA-TAB gives excellent capacity (2884 mg·g–1), ultrafast kinetics (60 min) distribution coefficient (Kd > 4 × 105 mL·g–1). More importantly, sterilization observed on both COF-TPTD-DHTA under visible light irradiation. This work provides paradigm develop ionic recovery.

Language: Английский

---