On Multicell-Interaction Chip: In Situ Observing the Interactions between the Astrocytes with Lysosomal Dysfunction and BBB Cells DOI
Yimeng Zhao, Chen Zhang,

Chaohui Liang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Lysosomes in astrocytes play vital roles toxic protein degradation the brain. Lysosomal dysfunction can lead to abnormal deposits, which further induce damage neurons and blood–brain barrier (BBB), thereby affect interaction between nervous vascular systems. Therefore, investigating interactions with lysosomal BBB cells is of significant importance. However, lack effective vitro models hinders study this complex system. Herein, an 8-well arrayed microfence multicell interculture chip (AMMIC) a hydrophilically optimized surface introduced for cells. Then, novel lysosome-targeted photosensitizer, IVQ-2Br, synthesized inducing controllable oxidative stress lysosomes astrocytes. By combination AMMIC model studying has been constructed. Particularly, severe secondary injuries brought about by stress, including alterations cell morphology activity as well notable DNA damage, are situ observed on AMMIC. The mediators involved injury-mediated intercellular communication validated be reactive oxygen species (ROS) exosomes. This work not only presents modeling method cell–cell but also demonstrates potential constructed through integration microfluidic techniques photosensitizers advancing biomedical research.

Language: Английский

Recent advances in single fluorescent probes for monitoring dual organelles in two channels DOI Creative Commons

Xiu‐Zhi Yang,

Shankun Yao,

Jisong Wu

et al.

Smart Molecules, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of organelle networks associated multiple pathological processes. However, clearly intuitively visualizing highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due distinct advantages facile, non‐invasiveness detection, making it particularly well‐suited applications in uncovering mysterious veil interactions. Regrettably, lack ideal fluorescence agents has always been an obstacle intricate behaviors organelles. In this review, we provide a systematic discussion on existing dual‐color dual‐targetable molecular sensors used monitoring interactions, focus their targeting strategies, mechanisms biological applications. Additionally, current limitations future development directions probes dual‐emissives briefly discussed. This review aims guidance researchers develop more improved studying biomedical field.

Language: Английский

Citations

1
On Multicell-Interaction Chip: In Situ Observing the Interactions between the Astrocytes with Lysosomal Dysfunction and BBB Cells DOI
Yimeng Zhao, Chen Zhang,

Chaohui Liang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Lysosomes in astrocytes play vital roles toxic protein degradation the brain. Lysosomal dysfunction can lead to abnormal deposits, which further induce damage neurons and blood–brain barrier (BBB), thereby affect interaction between nervous vascular systems. Therefore, investigating interactions with lysosomal BBB cells is of significant importance. However, lack effective vitro models hinders study this complex system. Herein, an 8-well arrayed microfence multicell interculture chip (AMMIC) a hydrophilically optimized surface introduced for cells. Then, novel lysosome-targeted photosensitizer, IVQ-2Br, synthesized inducing controllable oxidative stress lysosomes astrocytes. By combination AMMIC model studying has been constructed. Particularly, severe secondary injuries brought about by stress, including alterations cell morphology activity as well notable DNA damage, are situ observed on AMMIC. The mediators involved injury-mediated intercellular communication validated be reactive oxygen species (ROS) exosomes. This work not only presents modeling method cell–cell but also demonstrates potential constructed through integration microfluidic techniques photosensitizers advancing biomedical research.

Language: Английский

Citations

0