CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 7, 2023

The acceptance of new crop varieties by consumers is contingent on the presence consumer-preferred traits, which include sensory attributes, nutritional value, industrial products and bioactive compounds production. Recent developments in genome editing technologies provide novel insight to identify gene functions improve various qualitative quantitative traits commercial importance plants. Various conventional as well advanced gene-mutagenesis techniques such physical chemical mutagenesis, CRISPR-Cas9, Cas12 base editors are used for trait improvement crops. To meet consumer demand, breakthrough biotechnologies, especially CRISPR-Cas have received a fair share scientific interest, particularly plant editing. versatile tool that can be knock out, replace knock-in desired fragments at targeted locations genome, resulting heritable mutations interest. This review highlights existing literature recent (base editing, prime multiplex epigenome delivery methods) reliable precise also discusses potential exhibited crops consumer-demanded higher colour, texture, aroma/flavour, production biofuel, fibre, rubber pharmaceuticals. In addition, bottlenecks challenges associated with system, off targeting, ploidy level ability edit organelle been discussed.

Language: Английский

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Molecular and archaeological evidence on the geographical origin of domestication for Camelina sativa

American Journal of Botany, Journal Year: 2022, Volume and Issue: 109(7), P. 1177 - 1190

Published: June 18, 2022

Camelina (gold-of-pleasure or false flax) is an ancient oilseed crop with emerging applications in the production of sustainable, low-input biofuels. Previous domestication hypotheses suggested a European western Asian origin, yet little genetic evidence has existed to assess geographical origin for this crop, and archaeological data have not been systematically surveyed.We utilized genotyping-by-sequencing 185 accessions C. sativa its wild relatives examine population structure within species relationship populations progenitor, microcarpa; cytotype variation was also assessed both species. In complementary analysis, we surveyed literature identify sites archaeobotanical camelina remains timing prevalence usage across Europe Asia.The majority microcarpa sampled United States belongs variant (2n = 38) distinct evolutionary from that lineage 40). Populations Transcaucasia (South Caucasus) are most closely related based on structure; combination insights, these refute prior origin.Our findings support Caucasus, potentially Armenian, domestication. We cannot definitively determine whether intentionally targeted own right instead arose secondarily through selection agricultural traits weedy sativa, as originally proposed by Vavilov

Language: Английский

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Seed and Straw Characterization of Nine New Varieties of Camelina sativa (L.) Crantz

Land, Journal Year: 2023, Volume and Issue: 12(2), P. 328 - 328

Published: Jan. 25, 2023

Camelina sativa (L.) Crantz is a promising oilseed crop that has increased worldwide attention because of its agronomic characteristics and potential uses. From an agricultural point view, this plant can grow in different environments, providing good yield with low input requirements. In addition, camelina seeds contain high percentage oil (36–47%) protein (24–31%), making them interesting for food or energy industries. Nevertheless, cultivation not widespread Europe, particularly Spain. the present context global change search new sustainable crops, we are conducting two pilot projects aiming to confirm option crops semi-arid climates (especially central Spain, Madrid) find profitable varieties farmers. To reach our objective have used nine varieties, recently developed, characterize compare their seed content, straw chemical composition. Finally, preliminary results, determine which better properties be future research breeding programs. These results part larger study carrying out.

Language: Английский

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The identification of the missing maternal genome of the allohexaploid camelina (Camelina sativa)

The Plant Journal, Journal Year: 2022, Volume and Issue: 112(3), P. 622 - 629

Published: Aug. 2, 2022

SUMMARY Hexaploid camelina ( Camelina sativa ; 2 n = 6 x 40) is an important oilseed crop closely related to Arabidopsis. Compared other polyploid crops, the origin of three subgenomes has begun be unveiled only recently. While phylogenomic studies identified diploid C. hispida (2 14) as paternal genome , maternal donor remained unknown. Because chromosomes assigned a putative resembled those neglecta 12), tetraploid ‐like 4 26) was hypothesized likely ancestor hexaploid crop. Here we report chromosome‐level structure predicted among genotypes previously classified together microcarpa and referred here intermedia . Detailed cytogenomic analysis revealed high collinearity with two maternally inherited The identification missing provides new insights into reticulate evolutionary history complex allows us postulate comprehensive model for genus. herein elucidated opens door subsequent modifications resynthesis allohexaploid genome.

Language: Английский

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Overcoming genetic paucity of Camelina sativa: possibilities for interspecific hybridization conditioned by the genus evolution pathway

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 25, 2023

Camelina or false flax ( sativa ) is an emerging oilseed crop and a feedstock for biofuel production. This species believed to originate from Western Asian Eastern European regions, where the center of diversity genus located. Cultivated arose via series polyploidization events, serving as bottlenecks narrowing genetic species. The paucity C. foreseen most crucial limitation successful breeding improvement this crop. A potential solution challenge could be gene introgression wild resynthesized allohexaploid . However, both approaches would require complete comprehension evolutionary trajectories that led origin. Although there are some studies discussing origin evolution hexaploid species, final conclusions have not been made yet. Here, we propose integrated model based on recently described findings, which enables efficient interspecific hybridization with its relatives. We also discuss issues intergeneric hybridization, aimed improving overcoming proposed comprehensive indicates newly neglecta has key role in tetra- hexaploids, all two -based subgenomes. Understanding within provides insights into further research improvements resynthesis

Language: Английский

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Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments

Heliyon, Journal Year: 2024, Volume and Issue: 10(7), P. e27975 - e27975

Published: March 21, 2024

Spreng is a promising emerging oilseed crop, with its seed oil accounting for approximately 50% of the weight.

Language: Английский

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Exploring genetic diversity, population structure, and subgenome differences in the allopolyploid Camelina sativa: implications for future breeding and research studies

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(11)

Published: Sept. 9, 2024

Language: Английский

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REVISITING ARCHIVED RYE GRAINS DISCOVERED AT THE NEOLITHIC SITE CUNEŞTI (ROMANIA)

Radiocarbon, Journal Year: 2023, Volume and Issue: 65(3), P. 771 - 788

Published: April 14, 2023

ABSTRACT Direct accelerator mass spectrometry (AMS) dating is crucial for a correct integration of plant remains in the (pre)history crops, particularly those that do not belong to Neolithic package and are known arrive Europe much later. This paper reviews one earliest records rye from Romania. The grains were discovered tell settlement Cunești, which belongs Gumelnița communities (ca. 4600–3900 BC). In 1954, due Danube flooding, large portion south part collapsed, between burnt dwelling visible resulting profile, number sherds three typical pots identified. According excavation’s author, found association with sherds, it was assumed batch stored these Eneolithic vessels. Consequently, published as belonging period. Our reanalysis led two radiocarbon ( 14 C) dates, different laboratories, indicate Cunești prehistoric but dates medieval To this error concerning implications European archaeology, we decided republish an updated chronological framework. addition, performed stable isotope analyses on charred grains, confirming they cultivated dry land, well 3D morphometric investigation. research brings new original data cultures period southeastern Europe.

Language: Английский

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Assessing the productivity potential of camelina (Camelina sativa L. Crantz) in the Mediterranean basin: Results from multi-year and multi-location trials in Europe and Africa

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 219, P. 119080 - 119080

Published: June 29, 2024

Camelina is a multi-purpose oilseed crop for industrial bio-based applications. Recently, it has gained increasing attention mostly due to the possibility grow with limited resources and in marginal environments, as well increase soil coverage water retention, while reducing nitrogen leaching. The present study aimed assess viability of using camelina an emerging EU African Mediterranean farmers. Several spring genotypes were evaluated multi-location trial (Italy, Spain, Algeria, Morocco Tunisia) two growing seasons (2020–22). Although thermal time was very similar at all sites (∼1300 GDD from sowing harvest), cycle length about 60 days longer Europe than Africa, leading higher seed yield (∼ 1.5-fold higher). Mean production countries 0.71 Mg ha−1 proving be adaptable even arid conditions. Under more favorable conditions (European countries) yields up 2 ha−1. Among genotypes, Alba generally exhibited highest weight, CCE42 CCE29 showed stable productions over years locations. Based on results possible conclude that introduction into conventional cropping systems appeared feasible option improve diversification; however, selection best varieties essential achieve sustainable productions, particularly countries.

Language: Английский

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Biosystematics Studies of Endemic Thlaspi violascens Boiss. and T. densiflorum Boiss. & Kotschy: Highlights on Molecular and Morphological Identification

Flora, Journal Year: 2024, Volume and Issue: unknown, P. 152644 - 152644

Published: Nov. 1, 2024

Language: Английский

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