Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 15, 2024
Detecting
proteins
in
ultralow
concentrations
complex
media
is
important
for
many
applications
but
often
relies
on
complicated
techniques.
Herein,
a
single-molecule
protein
analyzer
with
the
potential
high-throughput
reported.
Gold-coated
magnetic
nanoparticles
DNA-labeled
antibodies
were
used
target
recognition
and
separation.
The
immunocomplex
was
loaded
into
microdroplets
generated
centrifugation.
Immuno-PCR
amplification
of
DNA
enabled
quantification
at
level
single
molecules.
As
an
example,
ultrasensitive
detection
α-synuclein,
biomarker
neurodegenerative
diseases,
achieved.
limit
determined
to
be
∼50
aM
buffer
∼170
serum.
method
exhibited
high
specificity
could
analyze
post-translational
modifications
such
as
phosphorylation.
This
study
will
inspire
wider
studies
detection,
especially
disease
diagnostics,
discovery,
drug
development.
Alzheimer s & Dementia,
Journal Year:
2023,
Volume and Issue:
20(1), P. 330 - 340
Published: Aug. 24, 2023
Abstract
BACKGROUND
Phosphorylated
tau
(p‐tau)
accumulation,
a
hallmark
of
Alzheimer's
disease
(AD),
can
also
be
found
in
the
retina.
However,
it
is
uncertain
whether
linked
to
AD
or
another
tauopathy.
METHODS
Retinas
from
164
individuals,
with
and
without
AD,
were
analyzed
for
p‐tau
accumulation
its
relationship
age,
dementia,
vision
impairment.
RESULTS
Retinal
pathology
showed
consistent
pattern
four
stages
molecular
composition
distinct
that
cerebral
tauopathies.
The
stage
retinal
correlated
age
(r
=
0.176,
P
0.024)
was
associated
(odds
ratio
[OR]
3.193;
0.001),
inflammation
(OR
2.605;
0.001).
Vision
impairment
underlying
eye
diseases
(β
0.292;
0.001)
0.192;
0.030)
linear
regression
model.
CONCLUSIONS
results
show
presence
primary
tauopathy
Alzheimer s & Dementia,
Journal Year:
2024,
Volume and Issue:
20(6), P. 4185 - 4198
Published: May 15, 2024
This
study
addresses
the
urgent
need
for
non-invasive
early-onset
Alzheimer's
disease
(EOAD)
prediction.
Using
optical
coherence
tomography
angiography
(OCTA),
we
present
a
choriocapillaris
model
sensitive
to
EOAD,
correlating
with
serum
biomarkers.
Journal of Clinical Medicine,
Journal Year:
2022,
Volume and Issue:
12(1), P. 152 - 152
Published: Dec. 24, 2022
The
retina
is
a
window
to
the
human
body.
Oculomics
study
of
correlations
between
ophthalmic
biomarkers
and
systemic
health
or
disease
states.
Deep
learning
(DL)
currently
cutting-edge
machine
technique
for
medical
image
analysis,
in
recent
years,
DL
techniques
have
been
applied
analyze
retinal
images
oculomics
studies.
In
this
review,
we
summarized
studies
that
used
models
images—most
published
date
involved
color
fundus
photographs,
while
others
focused
on
optical
coherence
tomography
images.
These
showed
some
variables,
such
as
age,
sex
cardiovascular
events,
could
be
consistently
robustly
predicted,
other
thyroid
function
blood
cell
count,
not
be.
DL-based
has
demonstrated
fascinating,
“super-human”
predictive
capabilities
certain
contexts,
but
it
remains
seen
how
these
will
incorporated
into
clinical
care
whether
management
decisions
influenced
by
lead
improved
outcomes.
Alzheimer s & Dementia Diagnosis Assessment & Disease Monitoring,
Journal Year:
2023,
Volume and Issue:
15(2)
Published: April 1, 2023
We
performed
a
systematic
review
and
meta-analysis
of
the
association
between
retinal
imaging
parameters
Alzheimer's
disease
(AD).PubMed,
EMBASE,
Scopus
were
systematically
searched
for
prospective
observational
studies.
Included
studies
had
AD
case
definition
based
on
brain
amyloid
beta
(Aβ)
status.
Study
quality
assessment
was
performed.
Random-effects
meta-analyses
standardized
mean
difference,
correlation,
diagnostic
accuracy
conducted.Thirty-eight
included.
There
weak
evidence
peripapillary
nerve
fiber
layer
thinning
optical
coherence
tomography
(OCT)
(p
=
0.14,
11
studies,
n
828),
increased
foveal
avascular
zone
area
OCT-angiography
0.18,
four
207),
reduced
arteriole
venule
vessel
fractal
dimension
fundus
photography
<
0.001
p
0.08,
respectively,
three
297)
among
cases.Retinal
appear
to
be
associated
with
AD.
Small
study
sizes
heterogeneity
in
methods
reporting
make
it
difficult
determine
utility
these
changes
as
biomarkers.We
(AD).We
only
included
which
cases
status.Several
biomarkers
but
clinical
is
uncertain.Studies
should
focus
biomarker-defined
use
methods.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 15, 2024
Detecting
proteins
in
ultralow
concentrations
complex
media
is
important
for
many
applications
but
often
relies
on
complicated
techniques.
Herein,
a
single-molecule
protein
analyzer
with
the
potential
high-throughput
reported.
Gold-coated
magnetic
nanoparticles
DNA-labeled
antibodies
were
used
target
recognition
and
separation.
The
immunocomplex
was
loaded
into
microdroplets
generated
centrifugation.
Immuno-PCR
amplification
of
DNA
enabled
quantification
at
level
single
molecules.
As
an
example,
ultrasensitive
detection
α-synuclein,
biomarker
neurodegenerative
diseases,
achieved.
limit
determined
to
be
∼50
aM
buffer
∼170
serum.
method
exhibited
high
specificity
could
analyze
post-translational
modifications
such
as
phosphorylation.
This
study
will
inspire
wider
studies
detection,
especially
disease
diagnostics,
discovery,
drug
development.
