Evaluation of Illumina and Oxford Nanopore Sequencing for the Study of DNA Methylation in Alzheimer’s Disease and Frontotemporal Dementia DOI Open Access
Lorenzo Pagano, Davide Lagrotteria, Alessandro Facconi

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4198 - 4198

Published: April 28, 2025

DNA methylation is a critical epigenetic mechanism involved in numerous physiological processes. Alterations patterns are associated with various brain disorders, including dementias such as Alzheimer’s disease (AD) and frontotemporal dementia (FTD). Investigating these alterations essential for understanding the pathogenesis progression of disorders. Among methods detecting methylation, sequencing one most widely employed. Specifically, two main approaches commonly used analysis: bisulfite single-molecule long-read sequencing. In this review, we compared performances CpG detection obtained using popular platforms, Illumina Oxford Nanopore (ON) Our comparison considers several factors, accuracy, efficiency, genomic regions, costs, wet-lab protocols, bioinformatics pipelines. We provide insights into strengths limitations both particular focus on their application research AD FTD.

Language: Английский

Evaluation of Illumina and Oxford Nanopore Sequencing for the Study of DNA Methylation in Alzheimer’s Disease and Frontotemporal Dementia DOI Open Access
Lorenzo Pagano, Davide Lagrotteria, Alessandro Facconi

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4198 - 4198

Published: April 28, 2025

DNA methylation is a critical epigenetic mechanism involved in numerous physiological processes. Alterations patterns are associated with various brain disorders, including dementias such as Alzheimer’s disease (AD) and frontotemporal dementia (FTD). Investigating these alterations essential for understanding the pathogenesis progression of disorders. Among methods detecting methylation, sequencing one most widely employed. Specifically, two main approaches commonly used analysis: bisulfite single-molecule long-read sequencing. In this review, we compared performances CpG detection obtained using popular platforms, Illumina Oxford Nanopore (ON) Our comparison considers several factors, accuracy, efficiency, genomic regions, costs, wet-lab protocols, bioinformatics pipelines. We provide insights into strengths limitations both particular focus on their application research AD FTD.

Language: Английский

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