Shock,
Journal Year:
2024,
Volume and Issue:
62(1), P. 119 - 126
Published: March 25, 2024
ABSTRACT
Background
:
It
is
reported
that
circVMA21
has
an
inhibition
effect
on
sepsis-induced
acute
kidney
injury
(AKI).
Therefore,
the
underlying
molecular
mechanisms
of
in
AKI
are
worthy
further
investigation.
Material
and
Methods
Lipopolysaccharide
(LPS)
was
used
to
induce
HK2
cell
injury.
CircVMA21,
miR-337-3p
ZEB2
expression
tested
by
qRT-PCR.
Cell
growth
detected
CCK8
assay,
EdU
flow
cytometry.
Protein
levels
were
examined
western
blot.
The
inflammatory
factors
oxidative
stress
markers
measured
evaluate
response
stress.
RNA
relationship
as
verified
dual-luciferase
reporter
RIP
pull-down
assay.
Results
CircVMA21
had
decreased
patients.
Overexpressed
alleviated
LPS-induced
inflammation,
apoptosis,
Moreover,
sponged
miR-337-3p,
targeted
ZEB2.
inhibitory
reversed
overexpression,
overexpression
abolished
promotion
Conclusions
could
inhibit
via
miR-337-3p/ZEB2
axis.
Journal of Orthopaedic Surgery and Research,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: April 15, 2024
Abstract
Background
Circular
RNAs
(circRNAs)
play
an
important
role
in
osteoarthritis
(OA).
However,
the
of
circRNA
OA
is
still
unclear.
Here,
we
explored
and
mechanism
circ_0044235
OA.
Methods
CHON-001
cells
were
treated
with
IL-1β
to
establish
model
vitro.
The
levels
circ_0044235,
miR-375
phosphoinositide
3-kinase
(PI3K)
regulatory
subunit
3
(PIK3R3)
detected
by
quantitative
real-time
PCR.
Cell
count
kit-8
assay
flow
cytometry
used
detect
cell
viability
apoptosis.
concentrations
inflammation
factors
determined
enzyme-linked
immunosorbent
assay.
Western
blot
was
protein
levels.
interaction
between
or
PIK3R3
analyzed
dual-luciferase
reporter
RNA
immunoprecipitation
Results
Circ_0044235
significantly
decreased
cartilage
tissue
IL-1β-treated
cells.
Overexpression
promoted
IL-1β-stimulated
inhibited
apoptosis,
inflammation,
extracellular
matrix
(ECM)
degradation.
In
analysis,
could
act
as
a
sponge
for
positively
regulate
expression.
addition,
ameliorated
effect
overexpression
on
IL-1β-mediated
injury.
inhibition
mitigated
IL-1β-induced
injury,
while
silencing
restored
effect.
Conclusion
knockdown
alleviated
chondrocytes
injury
regulating
miR-375/PIK3R3
axis,
confirming
that
might
be
potential
target
treatment.
Shock,
Journal Year:
2024,
Volume and Issue:
62(1), P. 119 - 126
Published: March 25, 2024
ABSTRACT
Background
:
It
is
reported
that
circVMA21
has
an
inhibition
effect
on
sepsis-induced
acute
kidney
injury
(AKI).
Therefore,
the
underlying
molecular
mechanisms
of
in
AKI
are
worthy
further
investigation.
Material
and
Methods
Lipopolysaccharide
(LPS)
was
used
to
induce
HK2
cell
injury.
CircVMA21,
miR-337-3p
ZEB2
expression
tested
by
qRT-PCR.
Cell
growth
detected
CCK8
assay,
EdU
flow
cytometry.
Protein
levels
were
examined
western
blot.
The
inflammatory
factors
oxidative
stress
markers
measured
evaluate
response
stress.
RNA
relationship
as
verified
dual-luciferase
reporter
RIP
pull-down
assay.
Results
CircVMA21
had
decreased
patients.
Overexpressed
alleviated
LPS-induced
inflammation,
apoptosis,
Moreover,
sponged
miR-337-3p,
targeted
ZEB2.
inhibitory
reversed
overexpression,
overexpression
abolished
promotion
Conclusions
could
inhibit
via
miR-337-3p/ZEB2
axis.
