Herb pair of Huangqi‐Danggui exerts anti‐tumor immunity to breast cancer by upregulating PIK3R1

Animal Models and Experimental Medicine, Journal Year: 2024, Volume and Issue: 7(3), P. 234 - 258

Published: June 1, 2024

Abstract Background According to traditional Chinese medicine (TCM), drugs supplementing the vital energy, Qi, can eliminate tumors by restoring host immunity. The objective of this study is investigate underlying immune mechanisms anti‐tumor activity associated with Qi‐supplementing herbs, specifically paired use Huangqi and Danggui. Methods Analysis compatibility regularity was conducted screen combination TCMs. Using MTT assay a transplanted tumor mice model, effects TCMs were investigated in vitro vivo. High content analysis flow cytometry then used evaluate cellular immunity, followed network pharmacology molecular docking dissect significant active compounds potential mechanisms. Finally, mechanism ingredients verified experiments. Results There an optimal Danggui that, administered as aqueous extract, activate immunity suppress more effective than each drug on its own Based analysis, PIK3R1 core target for combined Molecular shows 6 components extract (quercetin, jaranol, isorhamnetin, kaempferol, calycosin, suchilactone) that bind PIK3R1. Jaranol most important component against breast cancer. suchilactone/jaranol and, especially, suchilactone/kaempferol are key enhancement extract. Conclusions cancer individual alone.

Language: Английский

---

Astragalus injection inhibits the growth of osteosarcoma by activating cytotoxic T lymphocyte and targeting CTSL

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119607 - 119607

Published: March 1, 2025

Language: Английский

---

Shenqi granules enhance recovery from cerebral ischemia-reperfusion injury by modulating tryptophan and tyrosine metabolism and activating NFE2L2/NRF2

Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156623 - 156623

Published: March 10, 2025

Language: Английский

---

Exploring Novel Therapeutic Targets in Breast Cancer via Comprehensive Omics Profiling and Experimental Verification

Biology, Journal Year: 2025, Volume and Issue: 14(4), P. 405 - 405

Published: April 11, 2025

Background: Breast cancer is the leading cause of cancer-related deaths among women worldwide. Deciphering molecular mechanisms breast crucial for developing targeted therapeutic approaches. Methods: This study analyzed gene expression profiles from Gene Expression Omnibus (GEO) database to identify differentially expressed genes (DEGs) in cancer. Mendelian randomization (MR) analysis was then employed using publicly available eQTL databases evaluate potential causal relationships between these DEGs and Enrichment analyses were further conducted explore their functional significance. Furthermore, external validation co-expressed The Cancer Genome Atlas (TCGA) database. In vitro assays drug sensitivity performed on selected target validate roles pathogenesis treatment. Results: A total 1052 upregulated 1380 downregulated identified Additionally, MR revealed 12 significant potentially contributing pathogenesis. These primarily enriched lipid metabolism immune responses via regulating microRNA functions AMPK signaling. Validation through TCGA confirmed differential tissues. Strikingly, less-reported DNASE2 ATOH8 demonstrated involvement modulating proliferation, migration, invasion cells. Notably, several commonly used clinical drugs management, such as 5-Fluorouracil, exhibited dramatically increased expression. Conclusions: Our provides novel insights into basis identifies promising strategies this condition.

Language: Английский

---

Studying the effects of Saposhnikoviae Radix on the pharmacokinetic profiles of 10 bioactive compounds originating from Astragali Radix in rat plasma by UHPLC-QTRAP-MS/MS

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 118813 - 118813

Published: Sept. 1, 2024

Language: Английский

---