Discover Sustainability, Journal Year: 2025, Volume and Issue: 6(1)
Published: Feb. 19, 2025
Language: Английский
Exploration, Journal Year: 2024, Volume and Issue: 4(6)
Published: April 15, 2024
Abstract Over the last two decades, lipid nanoparticles (LNPs) have evolved as an effective biocompatible and biodegradable RNA delivery platform in fields of nanomedicine, biotechnology, drug delivery. They are novel bionanomaterials that can be used to encapsulate a wide range biomolecules, such mRNA, demonstrated by current successes COVID‐19 mRNA vaccines. Therefore, it is important provide perspective on LNPs for delivery, which further offers useful guidance researchers who want work RNA‐based LNP field. This first summarizes approaches preparation LNPs, followed introduction key characterization parameters. Then, vitro cell experiments study performance, including selection, viability, cellular association/uptake, endosomal escape, their efficacy, were summarized. Finally, vivo animal aspects administration, dosing safety, therapeutic efficacy discussed. The authors hope this offer valuable enter field help them understand crucial parameters demand.
Language: Английский
Citations
20
ACS Nano, Journal Year: 2024, Volume and Issue: 18(2), P. 1464 - 1476
Published: Jan. 4, 2024
The mRNA technology has emerged as a rapid modality to develop vaccines during pandemic situations with the potential protect against endemic diseases. success of in producing an antigen is dependent on ability deliver cells using vehicle, which typically consists lipid nanoparticle (LNP). Self-amplifying (SAM) synthetic platform that, besides encoding for interest, includes replication machinery amplification cells. Thus, SAM can generate many copies and prolong expression lower doses than those required conventional mRNA. This work describes morphology LNPs containing encapsulated (SAM LNPs), being three four times larger We show evidence that changes its conformational structure when LNPs, becoming more compact free form. A characteristic "bleb" observed lipid-rich core aqueous RNA-rich core, both surrounded by DSPC-rich shell. used SANS SAXS data confirm prevalent LNP two-core compartments where components are heterogeneously distributed between two cores capped cylinder core–shell model interior was built capture overall LNP. These findings provide bleb two-compartment structures be representative highlight need additional studies elucidate role spherical morphologies, their mechanisms formation, parameters lead particular rational design delivery.
Language: Английский
Citations
19
Journal of Molecular Liquids, Journal Year: 2025, Volume and Issue: unknown, P. 126999 - 126999
Published: Jan. 1, 2025
Language: Английский
Citations
4
ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 8, 2025
Nanomedicine is revolutionizing precision medicine, providing targeted, personalized treatment options. Lipid-based nanomedicines offer distinct benefits including high potency, targeted delivery, extended retention in the body, reduced toxicity, and lower required doses. These characteristics make lipid-based nanoparticles ideal for drug delivery areas such as gene therapy, cancer treatment, mRNA vaccines. However, traditional bulk synthesis methods LNPs often produce larger particle sizes, significant polydispersity, low encapsulation efficiency, which can reduce therapeutic effectiveness. issues primarily result from uneven mixing limited control over formation during synthesis. Microfluidic technology has emerged a solution, precise size, uniformity, efficiency. In this mini review, we introduce state-of-the-art microfluidic systems nanoparticle functionalization. We include working principles of different types systems, use LNP synthesis, cargo encapsulation, nanomedicine delivery. end, briefly discuss clinical enabled by devices.
Language: Английский
Citations
2
Discover Sustainability, Journal Year: 2025, Volume and Issue: 6(1)
Published: Feb. 19, 2025
Language: Английский
Citations
2