Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(31), P. 12784 - 12793
Published: July 27, 2024
The
viscosity
that
ensures
the
controlled
diffusion
of
biomolecules
in
cells
is
a
crucial
biophysical
parameter.
Consequently,
fluorescent
probes
capable
reporting
variations
are
valuable
tools
bioimaging.
In
this
field,
red-shifted
essential,
as
widely
used
and
gold
standard
probe
remains
green-emitting
molecular
rotors
based
on
BODIPY.
Here,
we
demonstrate
pyrrolyl
squaraines,
red-emissive
fluorophores,
exhibit
high
sensitivity
over
wide
range
from
30
to
4890
mPa·s.
Upon
alkylation
pyrrole
moieties,
improve
their
through
an
enhanced
twisted
intramolecular
charge
transfer
phenomenon.
We
utilized
scaffold
develop
plasma
membrane
probe,
pSQ-PM,
efficiently
stains
fluorogenic
manner.
Using
fluorescence
lifetime
imaging,
pSQ-PM
enabled
efficient
sensing
under
various
conditions
different
cell
lines
(HeLa,
U2OS,
NIH/3T3).
Moreover,
upon
incubation,
stained
intracellular
vesicles
suggested
lysosomal
membranes
displayed
fluidity.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(14), P. 5437 - 5445
Published: March 26, 2024
The
research
on
fluorescent
rotors
for
viscosity
has
attracted
extensive
interest
to
better
comprehend
the
close
relationships
of
microviscosity
variations
with
related
diseases.
Although
scientists
have
made
great
efforts,
probes
cellular
both
aggregation-induced
emissions
(AIEs)
and
large
Stokes
shifts
improve
sensing
properties
rarely
been
reported.
Herein,
we
first
report
four
new
meso-C═N-substituted
BODIPY-based
shifts,
investigate
their
viscosity/AIE
characteristics,
perform
imaging
in
subcellular
organelles.
Interestingly,
meso-C═N-phenyl
group-substituted
probe
6
showed
an
obvious
594
nm
fluorescence
enhancement
glycerol
a
moderate
650
red
AIE
emission
water.
Further,
attaching
CF3
phenyl
group,
similar
phenomenon
was
observed
7
red-shifted
emissions,
attributed
introduction
which
plays
key
role
shifts.
Comparatively,
phenyl-group-free
probes,
meso-C═N-trifluoroethyl
group
thiazole-substituted
(8
9)
exhibited
good
viscosity-responsive
properties,
while
no
due
absence
groups.
For
experiments,
9
lysosomal
mitochondrial
targeting
respectively,
were
further
successfully
used
through
preincubation
monensin
lipopolysaccharide
(LPS),
indicating
that
C═N
polar
groups
potentially
work
as
rotatable
moieties
organelle-targeting
groups,
difference
might
be
ascribed
increased
charges
thiazole.
Therefore,
this
study,
investigated
structural
meso-C═N
respect
subcellular-targeting
ability,
viscosity,
serving
imaging.
The Journal of Physical Chemistry Letters,
Journal Year:
2024,
Volume and Issue:
15(25), P. 6685 - 6690
Published: June 20, 2024
Thioflavin
T
(ThT)
informed
microviscosity
changes
can
be
used
to
monitor
protein
aggregation.
Steady-state,
time-resolved
and
lasing
spectroscopy
were
detect
transient
states
in
α-synuclein
-
a
associated
with
Parkinson's
disease.
The
major
focus
was
on
the
nucleation
phase,
where
conventional
ThT
fluorescence
assay
lacks
appropriate
sensitivity
early
stage
oligomers.
Instead,
threshold
parameters,
particular,
sensitive
detecting
Through
spectroscopy,
change
correlating
stages
of
aggregation
observed
at
two
wavelengths
405
440
nm.
are
free
dye
molecules
β-sheet
bound
molecules.
This
provides
perspective
elucidating
formed
aggregation,
critical
aspect
understanding
pathogenesis
neurodegenerative
diseases.
insights
from
presented
study
shows
potential
using
as
tool
studying
dynamics.
Genetically
encoded
biosensors
based
on
fluorescent
proteins
are
valuable
tools
for
imaging
biological
processes
with
high
selectivity.
In
particular,
pH-sensitive
such
as
the
GFP-derived
superecliptic
pHluorin
(SEP)
or
pHuji
allow
tracking
of
protein
trafficking
through
acidic
and
neutral
compartments.
Recently,
chemogenetic
indicators
combining
synthetic
fluorophores
genetically
self-labeling
tags
(SLP-tag)
offer
a
versatile
alternative
that
combines
diversity
chemical
probes
selectivity
genetic-encoding.
Here,
we
describe
novel
fluorogenic
pH
sensor
consisting
cell-permeable
molecular
indicator
called
pHluo-Halo-1
whose
fluorescence
can
be
locally
activated
in
cells
by
reaction
SLP-tag
HaloTag
ensuring
signal
wash-free
experiments.
pHluo-Halo
display
good
sensitivity
suitable
pKa
5.9
to
monitor
variations.
This
hybrid
probe
was
applied
follow
exocytosis
CD63-HaloTag
fusion
enabling
visualization
exosomes
released
from
vesicles
into
extracellular
media
using
TIRF
microscopy.
platform
is
expected
powerful
tool
elucidating
dynamics
regulatory
mechanisms
living
cells.
Genetically
encoded
biosensors
based
on
fluorescent
proteins
are
valuable
tools
for
imaging
biological
processes
with
high
selectivity.
In
particular,
pH-sensitive
such
as
the
GFP-derived
superecliptic
pHluorin
(SEP)
or
pHuji
allow
tracking
of
protein
trafficking
through
acidic
and
neutral
compartments.
Recently,
chemogenetic
indicators
combining
synthetic
fluorophores
genetically
self-labeling
tags
(SLP-tag)
offer
a
versatile
alternative
that
combines
diversity
chemical
probes
selectivity
genetic-encoding.
Here,
we
describe
novel
fluorogenic
pH
sensor
consisting
cell-permeable
molecular
indicator
called
pHluo-Halo-1
whose
fluorescence
can
be
locally
activated
in
cells
by
reaction
SLP-tag
HaloTag
ensuring
signal
wash-free
experiments.
pHluo-Halo
display
good
sensitivity
suitable
pKa
5.9
to
monitor
variations.
This
hybrid
probe
was
applied
follow
exocytosis
CD63-HaloTag
fusion
enabling
visualization
exosomes
released
from
vesicles
into
extracellular
media
using
TIRF
microscopy.
platform
is
expected
powerful
tool
elucidating
dynamics
regulatory
mechanisms
living
cells.
Helvetica Chimica Acta,
Journal Year:
2024,
Volume and Issue:
107(7)
Published: May 9, 2024
Abstract
When
developing
fluorescent
membrane
probes,
we
naturally
tend
to
focus
on
the
fluorophore
itself.
In
this
study,
show
that
sometimes
it
can
be
beneficial
shift
attention
from
center
periphery,
maximize
multiple
interfacing
with
complex
changing
environments.
Palmitylation
for
hydrophobic
and
glutamate
dendrons
hydrophilic
are
combined
improve
performance
of
flipper
probes.
We
increase
in
membranes,
solubility
water
is
important,
water,
hydrophobicity
probe.
These
seemingly
paradoxical
measures
taken
satisfy
Israelachvili
rules.
They
state
only
inverted
cone
amphiphiles
form
soluble
micelles
while
shaped
precipitate
into
hexagonal
1
supramolecular
polymers,
intermediate
cylindrical
behavior
dominated
by
bilayers
lamellar
precipitates.
The
normal
obtained
prevent
precipitation
prepare
efficient
transfer
lipid
bilayer
membranes.
results
flippers
break
all
records
set
2016
original
regard
effective
brightness,
responsiveness
order,
anchoring
disordered
partitioning
membranes
high
stable
labeling
interest.
lessons
learned
confirm
obvious:
rules
apply
also
promise
literally
bright
perspectives
Physchem,
Journal Year:
2024,
Volume and Issue:
4(2), P. 157 - 166
Published: May 27, 2024
Rates
of
fast
reactions
are
inversely
proportional
to
the
solvent
viscosity
(η).
However,
a
quantitative
study
demonstrates
that
dynamic
η
is
often
crude
reflection
viscous
drug
exerted
on
molecule
or
radical.
This
paper
aims
present
an
accurate
dependence
rates
bi-
and
monomolecular
upon
media.
Different
correction
coefficients
fmicro
discussed,
which
should
lead
rate
∝
(fmicroη)−1.
Microviscosity
viscosity,
leading
expected
shear
viscosity.
In
many
cases,
experimentally
measured
diffusion
molecules
similar
structure
reactive
radicals
correct
prediction
radicals’
constants
recombination.
complex
non-Newtonian
liquids
(biological
liquids,
polymeric
solutions)
can
be
using
low
MW
molecular
probes.
Usually,
ηmicro
much
lower
than
biological
liquids.
Cis–trans
isomerization
bulky
groups
in
described
with
Kramers’
theory.
An
example
such
cyanine
dye
studied
theoretically
presented.
It
demonstrated
selected
case
theory
adequately
describes
cis–trans
organic
compounds
η.
