Mannose/stearyl chloride doubly functionalized polyethylenimine as a nucleic acid vaccine carrier to promote macrophage uptake DOI Creative Commons

Lu Bai,

Xiaoqi Chen,

Chengyu Li

et al.

Drug Delivery, Journal Year: 2024, Volume and Issue: 31(1)

Published: Nov. 14, 2024

Transmembrane transport remains a significant challenge for nucleic acid vaccine vectors. Promoting the ability of immune cells, such as macrophages, to capture foreign stimuli is also an effective approach improving cross-presentation. In addition, polyethyleneimine (PEI) has gained attention in field carriers due its excellent gene transfection efficiency and unique proton buffering effect. However, although high molecular weight PEI exhibits efficiency, high-density positive charges make it highly toxic, which limits application. this study, mannose/stearyl chloride functionalized polyethylenimine (SA-Man-PEI) was prepared by functionalizing (molecular 25 kDa) with mannose immunomodulatory phagocyte targeting effects, alkyl hydrophobic chain segment, could easily promote cell uptake. Moreover, functionalized-PEI retains strong effect, helps carrier escape from lysosome. The particle sizes composite particles formed SA-Man-PEI ovalbumin (OVA) were below 200 nm, good storage stability at both 4 °C 37 °C. At drug concentration 2 μg/mL, survival rate 19.2% higher than that unfunctionalized PEI. vitro macrophage endocytosis experiments showed significantly enhance uptake particles, compared or single-functionalized This study offers new developing carrier, simultaneously

Language: Английский

Mannose/stearyl chloride doubly functionalized polyethylenimine as a nucleic acid vaccine carrier to promote macrophage uptake DOI Creative Commons

Lu Bai,

Xiaoqi Chen,

Chengyu Li

et al.

Drug Delivery, Journal Year: 2024, Volume and Issue: 31(1)

Published: Nov. 14, 2024

Transmembrane transport remains a significant challenge for nucleic acid vaccine vectors. Promoting the ability of immune cells, such as macrophages, to capture foreign stimuli is also an effective approach improving cross-presentation. In addition, polyethyleneimine (PEI) has gained attention in field carriers due its excellent gene transfection efficiency and unique proton buffering effect. However, although high molecular weight PEI exhibits efficiency, high-density positive charges make it highly toxic, which limits application. this study, mannose/stearyl chloride functionalized polyethylenimine (SA-Man-PEI) was prepared by functionalizing (molecular 25 kDa) with mannose immunomodulatory phagocyte targeting effects, alkyl hydrophobic chain segment, could easily promote cell uptake. Moreover, functionalized-PEI retains strong effect, helps carrier escape from lysosome. The particle sizes composite particles formed SA-Man-PEI ovalbumin (OVA) were below 200 nm, good storage stability at both 4 °C 37 °C. At drug concentration 2 μg/mL, survival rate 19.2% higher than that unfunctionalized PEI. vitro macrophage endocytosis experiments showed significantly enhance uptake particles, compared or single-functionalized This study offers new developing carrier, simultaneously

Language: Английский

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