Classification of polyphenol oxidases shows ancient gene duplication leading to two distinct enzyme types

iScience, Journal Year: 2025, Volume and Issue: 28(2), P. 111771 - 111771

Published: Jan. 10, 2025

Polyphenol oxidases (PPOs) are coupled binuclear copper proteins that catalyze the oxidation of phenols. New functions PPOs continuously being discovered, latest with several fungal o-methoxy phenolases, which active on lignin-derived compounds. Here, we perform a comprehensive phylogenetic analysis from wide taxonomic origin and define 12 PPO groups. We find deep gene duplication has led to two distinct types. Type 1 includes chordates molluscs, as well phenolases. 2 plant PPOs, molluscan hemocyanins, tyrosinases. Most type have C-terminal shielding domain thioether bond in copper-binding site. also most ascomycetes contain high numbers may indicate role lignin conversion strategy these fungi.

Language: Английский

Recent advances in enzymes active on lignin-derived aromatic compounds

Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Reducing lignin condensation and enhancing enzymatic hydrolysis of wheat straw by low-concentration p-toluenesulfonic acid pretreatment assisted with polyethylene glycol

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162012 - 162012

Published: March 1, 2025

Language: Английский