Helicity‐Dependent Enzymatic Peptide Cyclization

Journal of Peptide Science, Journal Year: 2025, Volume and Issue: 31(6)

Published: April 27, 2025

ABSTRACT The secondary structure plays a crucial role in the biological activity of peptides. Various strategies have been developed to stabilize particular peptide conformations, including sequence modifications and macrocyclization approaches. Often, interplay between conformational constraint flexibility is central bioactivity. Here, we investigate how α‐helicity influences enzymatic head‐to‐tail cyclization using an engineered Sortase. We show that peptides with low helicity readily undergo intramolecular cyclization, while more rigid, helical exhibit complex behaviors cyclic dimer formation. These findings reveal increased rigidity can redirect reactions from intermolecular processes, demonstrates changes molecular guide chemical reactivity. insights advance design peptide‐derived materials, hydrogels, stimuli‐responsive probes.

Language: Английский

Can Kisqali® (Ribociclib) effectively target triple-negative breast cancer? A computational insight on potential mechanisms and therapeutic strategies

Discover Chemistry., Journal Year: 2025, Volume and Issue: 2(1)

Published: May 9, 2025

Language: Английский