Spatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective ncRNA (e.g., microRNA (miRNA)) mitochondria via an organelle-specific DNA assembly strategy. The target miRNA-initiated hairpins is inhibited by the block toehold-mediated strand displacement reaction can be exclusively activated mitochondria-encoded ribosomal RNA (rRNA) hybridization chain reaction, enabling spatial control over miRNA imaging. We demonstrate that conditionally controlled technology minimization nonspecific activation and thus improves precision detection. In addition, strategy adaptable to visualizing other ncRNAs such as long mitochondria, highlighting universality approach. Overall, this work provides useful tool investigating organelle-located RNA.

Language: Английский

A versatile biosensor for detecting Zn2+ and Cu2+ in Alzheimer's disease cells and acidic foods via RNA G-quadruplex hydrolysis

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 490, P. 137847 - 137847

Published: March 5, 2025

Language: Английский

Signal Transduction Strategies for DNAzyme-Based Sensing and Imaging of Metal Ions in Cells and in Vivo

Chemical & Biomedical Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Language: Английский