Angewandte Chemie International Edition,
Journal Year:
2021,
Volume and Issue:
60(28), P. 15426 - 15435
Published: April 1, 2021
Abstract
A
general
strategy
to
carry
out
cell
uptake
and
biodistribution
studies
is
label
nanoparticles
(NPs)
with
a
fluorescent
dye.
However,
the
comparative
study
of
different
dye‐loaded
NPs
remains
difficult
owing
uncontrolled
dye
quenching
de‐quenching.
Here
we
compared
two
types
dye‐labeled
demonstrated
their
distinct
properties.
molecules
at
solid
state
suffer
from
quenching,
so
release
and/or
NP
degradation
in
biological
environments
leads
several‐fold
increase
fluorescence
intensity
despite
same
amount
NPs,
switch
In
contrast,
soluble
exhibit
no
effect.
To
standardize
study,
propose
possible
solutions:
using
lower
loading
or
medium
analysis
for
quantifying
NPs.
This
work
provides
valuable
insights
into
selecting
valid
quantification
methods
bio‐nano
studies.
Aggregate,
Journal Year:
2023,
Volume and Issue:
4(2)
Published: Jan. 12, 2023
Abstract
Increasing
drug
loading
remains
a
critical
challenge
in
the
development
and
translation
of
nanomedicine.
High
drug‐loading
nanoparticles
have
demonstrated
unique
advantages
such
as
less
carrier
material
used,
better‐controlled
release,
improved
efficacy
safety.
Herein,
we
report
simple
efficient
salt
concentration
screening
method
for
making
polymer
with
exceptionally
high
(up
to
66.5
wt%)
based
on
phase
separation‐induced
nanoprecipitation.
Upon
addition
salt,
separation
occurs
miscible
solvent‐water
solution
delaying
precipitation
time
drugs
polymers
different
extents,
facilitating
their
co‐precipitation
thus
formation
encapsulation
efficiency
(>90%)
excellent
stability
(>1
month).
This
technology
is
versatile
easy
be
adapted
various
hydrophobic
drugs,
polymers,
solvents.
salt‐induced
nanoprecipitation
strategy
offers
novel
approach
fabricating
tunable
loading,
opens
great
potentials
future
nanomedicines.
Chemical Science,
Journal Year:
2023,
Volume and Issue:
14(33), P. 8823 - 8830
Published: Jan. 1, 2023
Development
of
robust
multi-color
photoswitchable
fluorescent
probes
is
critical
for
many
optical
applications,
but
it
remains
a
challenge
to
rationally
design
these
probes.
Here,
we
report
new
Förster
resonance
energy
transfer-based
dual-color
nanoparticles
(DPF
NPs)
by
taking
advantage
the
distinct
properties
ligand-protected
gold
nanoclusters
(AuNCs).
Detailed
photophysical
studies
revealed
that
ultrasmall-sized
AuNCs
not
only
act
as
FRET
donors
due
their
intrinsic
fluorescence
properties,
also
play
significant
role
in
regulating
photochromic
and
aggregate
spiropyran
through
ligand-spiropyran
interactions.
These
DPF
NPs
exhibit
high
on/off
ratio
(∼90%)
both
green
red
emission,
good
reversibility
during
cycled
photo-stimulation.
Cell
imaging
experiments
showed
could
specifically
accumulate
lipid
droplets,
enable
living
cells.
Moreover,
labeling
mitochondria
with
green-emitting
marker,
demonstrated
can
distinguish
different
targets
based
on
dynamic
static
signals
at
sub-cellular
level
two
emission
channels
reliably.
This
study
provides
strategy
designing
modulating
dyes
nanoclusters,
which
be
generalized
development
other
photoswitch
systems
towards
advanced
applications.
Biosensors,
Journal Year:
2021,
Volume and Issue:
11(12), P. 505 - 505
Published: Dec. 9, 2021
Fluorescence
labelling
is
often
used
for
tracking
nanoparticles,
providing
a
convenient
assay
monitoring
nanoparticle
drug
delivery.
However,
it
difficult
to
be
quantitative,
as
many
factors
affect
the
fluorescence
intensity.
Förster
resonance
energy
transfer
(FRET),
taking
advantage
of
from
donor
fluorophore
an
acceptor
fluorophore,
provides
distance
ruler
probe
NP
This
article
review
different
FRET
approaches
ratiometric
self-assembly
and
formation
their
in
vivo
fate,
integrity
release.
We
anticipate
that
fundamental
understanding
gained
these
studies
will
offer
new
insights
into
design
nanoparticles
with
improved
better-controlled
properties.
Macromolecular Rapid Communications,
Journal Year:
2022,
Volume and Issue:
43(24)
Published: June 11, 2022
Fluorescence
resonance
energy
transfer
(FRET)
is
a
non-invasive
characterization
method
for
studying
molecular
structures
and
dynamics,
providing
high
spatial
resolution
at
nanometer
scale.
Over
the
past
decades,
FRET-based
measurements
are
developed
widely
implemented
in
synthetic
polymer
systems
understanding
detecting
variety
of
nanoscale
phenomena,
enabling
significant
advances
science.
In
this
review,
basic
principles
fluorescence
FRET
briefly
discussed.
Several
representative
research
areas
highlighted,
where
spectroscopy
imaging
can
be
employed
to
reveal
morphology
kinetics.
These
examples
include
micelle
formation
stability,
guest
molecule
release
from
host,
characterizing
supramolecular
assembly,
composite
interfaces,
determining
chain
conformations
their
diffusion
Finally,
perspective
on
opportunities
provided
further
allowing
greater
contributions
exciting
area.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(12), P. 5006 - 5013
Published: March 14, 2024
The
development
of
new
imaging
and
treatment
nanoprodrug
systems
is
highly
demanded
for
diagnosis
therapy
liver
cancer,
a
severe
disease
characterized
by
high
recurrence
rate.
Currently,
available
small
molecule
drugs
are
not
possible
cancer
because
the
fast
diffusion
agents
low
efficacy
in
due
to
poor
water
solubility
significant
toxic
side
effects.
In
this
study,
we
report
tumor
microenvironment
activatable
system
cancer.
This
can
accumulate
site
be
selectively
activated
an
excess
hydrogen
peroxide
(H2O2)
microenvironment,
releasing
near-infrared
solid-state
organic
fluorescent
probe
(HPQCY-1)
phenylboronic
acid-modified
camptothecin
(CPT)
prodrug.
Both
HPQCY-1
CPT
prodrugs
further
sites
achieving
more
precise
situ
(NIR)
fluorescence
while
reducing
effects
on
normal
tissues.
Additionally,
incorporation
hydrophilic
multivalent
chitosan
as
carrier
effectively
improved
system.
research
thus
provides
practical
approach
Aggregate,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 3, 2024
Abstract
Single‐drug
therapies
or
monotherapies
are
often
inadequate,
particularly
in
the
case
of
life‐threatening
diseases
like
cancer.
Consequently,
combination
emerge
as
an
attractive
strategy.
Cancer
nanomedicines
have
many
benefits
addressing
challenges
faced
by
small
molecule
therapeutic
drugs,
such
low
water
solubility
and
bioavailability,
high
toxicity,
etc.
However,
it
remains
a
significant
challenge
encapsulating
two
drugs
nanoparticle.
To
address
this
issue,
computational
methodologies
employed
to
guide
rational
design
synthesis
dual‐drug‐loaded
polymer
nanoparticles
while
achieving
precise
control
over
drug
loading.
Based
on
sequential
nanoprecipitation
technology,
five
factors
identified
that
affect
formulation
candidates
into
dual‐drug
loaded
nanoparticles,
then
screened
176
formulations
under
different
experimental
conditions.
these
data,
machine
learning
methods
applied
pin
down
key
factors.
The
implementation
methodology
holds
potential
significantly
mitigate
complexities
associated
with
co‐assembly
compounds
nanoparticulate
systems
demonstrates
promising
avenue
for
therapy.
This
approach
provides
new
strategy
enabling
streamlined,
high‐throughput
screening
nanoscale
drug‐loaded
entities.
Advanced Science,
Journal Year:
2023,
Volume and Issue:
11(6)
Published: Dec. 28, 2023
Abstract
Deep
NIR
organic
phototheranostic
molecules
generally
have
large
π‐conjugation
structures
and
show
highly
hydrophobic
properties,
thus,
forming
strong
π–π
stacking
in
the
aqueous
medium,
which
will
affect
performance.
Herein,
an
end‐group
strategy
is
developed
to
lift
performance
of
NIR‐II
emitting
photosensitizers.
Extensive
characterizations
reveal
that
hydrogen‐bonding
interactions
hydroxyl
end
group
can
induce
a
more
intense
electronic
coupling
than
chlorination‐mediated
intermolecular
forces.
The
results
disclose
lower
fluorescence
quantum
yield
but
significantly
benefit
photodynamic
therapy
(PDT)
efficiency.
Accordingly,
asymmetrically
substituted
derivative
(BTIC‐
δ
OH‐2Cl)
developed,
shows
balanced
properties
with
excellent
PDT
efficiency
(14.6
folds
ICG)
high
(2.27%).
It
proves
validity
on
controlling
rational
tuning
agents.
