Rationally Designed Cell Membrane Biomimetic Biosensing Platform for the Binding Analysis of Drugs with Intracellular Kinase Domain of Epidermal Growth Factor Receptor DOI
Xia Liu, Quan Feng, Qi Hu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Biosensing technologies have demonstrated significant potential in exploring the binding of drugs to receptor tyrosine kinases (RTKs). As a typical transmembrane receptor, there are still several shortcomings utilization intracellular kinase domain RTKs, primary action site small-molecule inhibitors, resulting insufficient and unclear sites, which impair efficiency accuracy biosensing. Herein, using epidermal growth factor (EGFR) as an example, we reported biosensing platform based on cell membrane camouflage technology for evaluating EGFR. The azide-functionalized membranes modified through glucose metabolism were reverse-coated onto alkyne-functionalized magnetic nanoparticles via bioorthogonal reaction (CMRMNPs), therefore effectively exposing EGFR without damage. To construct platform, fluorescent probe derived from gefitinib pharmacophore (GN probe) was further synthesized incubated with CMRMNPs. This strategy facilitated efficient localization GN within Ultimately, this approach successfully implemented evaluate three inhibitors study provides viable constructing biomimetic biosensors defined orientation offers novel insights methodologies drug regions RTKs.

Language: Английский

Synthesis, properties and emerging applications of multi-boron coordinated chromophores DOI

Chaochao Jin,

Xuan Yang, Wenjing Zhao

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 513, P. 215892 - 215892

Published: May 6, 2024

Language: Английский

Citations

8
Quantitative analysis of disaggregation properties of aggregation‐induced emission luminogens (AIEgens) and off‐the‐shelf dyes DOI Creative Commons

Kyung Tae Hong,

J.Y. Jeon,

Soeun Park

et al.

Bulletin of the Korean Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Abstract The aggregation state of chemosensors significantly influences their photophysical properties, making it critical for applications in sensing and imaging. In this study, we systematically investigated the disaggregation properties 11 representative fluorophores, including widely used dyes such as BODIPY, coumarin, fluorescein, rhodamine B, Nile Red, well AIEgens like TPE TPA. Through a quantitative approach, determined concentration (DC₅₀) various aqueous‐organic co‐solvent mixtures correlated these values with solvent polarity parameters, dielectric constant E T (30). Our analysis uncovered distinct DC₅₀ patterns that act unique fingerprints each compound, reflecting structural characteristics. Notably, structurally similar compounds, BODIPY derivatives dansyl‐based chemosensors, exhibited comparable trends. This study underscores utility versus scatter plots evaluating aggregation‐dependent offers valuable insights into molecular determinants self‐aggregation, enabling more rational chemosensor design.

Language: Английский

Citations

0
Rationally Designed Cell Membrane Biomimetic Biosensing Platform for the Binding Analysis of Drugs with Intracellular Kinase Domain of Epidermal Growth Factor Receptor DOI
Xia Liu, Quan Feng, Qi Hu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Biosensing technologies have demonstrated significant potential in exploring the binding of drugs to receptor tyrosine kinases (RTKs). As a typical transmembrane receptor, there are still several shortcomings utilization intracellular kinase domain RTKs, primary action site small-molecule inhibitors, resulting insufficient and unclear sites, which impair efficiency accuracy biosensing. Herein, using epidermal growth factor (EGFR) as an example, we reported biosensing platform based on cell membrane camouflage technology for evaluating EGFR. The azide-functionalized membranes modified through glucose metabolism were reverse-coated onto alkyne-functionalized magnetic nanoparticles via bioorthogonal reaction (CMRMNPs), therefore effectively exposing EGFR without damage. To construct platform, fluorescent probe derived from gefitinib pharmacophore (GN probe) was further synthesized incubated with CMRMNPs. This strategy facilitated efficient localization GN within Ultimately, this approach successfully implemented evaluate three inhibitors study provides viable constructing biomimetic biosensors defined orientation offers novel insights methodologies drug regions RTKs.

Language: Английский

Citations

0