Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase‐2 Activity via an Energy Diversion Process DOI

Weijing Huang,

Wenhui Zeng, Zheng Huang

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(26)

Published: April 19, 2024

Abstract Ratiometric afterglow luminescent (AGL) probes are attractive for in vivo imaging due to their high sensitivity and signal self‐calibration function. However, there currently few ratiometric AGL available enzymatic activity living organisms. Here, we present an energy diversion (ED) strategy that enables the design of enzyme‐activated probe (RAG‐RGD) imaging. The ED process provides RAG‐RGD with a radiative transition ‘always on’ 520‐nm (AGL 520 ) cascade three‐step transfer (ET) ‘off‐on’ 710‐nm 710 response specific enzyme. Using matrix metalloproteinase‐2 (MMP‐2) as example, shows significant ~11‐fold increase /AGL toward MMP‐2. This can sensitively detect U87MG brain tumors through MMP‐2 activity, signal‐to‐background ratio deep depth. Furthermore, by utilizing effect imaging, demonstrated strong negative correlation between value size orthotopic tumor, enabling accurate monitoring glioma growth vivo. may be applied other sensitive

Language: Английский

Highly Photoreactive Semiconducting Polymers with Cascade Intramolecular Singlet Oxygen and Energy Transfer for Cancer-Specific Afterglow Theranostics DOI
Yiguang Lin, Jingsheng Huang, Jing Liu

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Afterglow luminescence provides ultrasensitive optical detection by minimizing tissue autofluorescence and increasing the signal-to-noise ratio. However, due to lack of suitable unimolecular afterglow scaffolds, current agents are nanocomposites containing multiple components with limited performance have rarely been applied for cancer theranostics. Herein, we report synthesis a series oxathiine-containing donor-acceptor block semiconducting polymers (PDCDs) observation their high photoreactivity strong near-infrared (NIR) luminescence. We reveal that PDCDs absorb NIR light undergo photodynamic process generate singlet oxygen (1O2), which intramolecularly transfers efficiently reacts oxathiine form intermediates low Gibbs free energy changes required this photoreaction. Following intramolecular transfer from donor acceptor block, emission is produced PDCDs. Owing efficient cascade photochemical process, PDCD-based nanoparticles achieve higher brightness longer compared most reported agents, even after ultrashort photoirradiation only 3 s. Furthermore, within PDCD can be inhibited bioconjugation quencher-linked peptide. This allows construction cancer-activatable theranostic probe (CATP) switches on signal function in presence cancer-overexpressed enzyme. Thereby, CATP represents first phototheranostic permits cancer-specific therapy under preclinical settings. In summary, study molecular guideline develop probes photoreactive polymers.

Language: Английский

Citations

0
A highly effective self-supplying photosensitizer drug for deep-tissue metastatic tumours treatment DOI Creative Commons
Zhengze Yu, Hanxiang Li, Mingchao Xia

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Due to the inherent defects of photodynamic therapy (PDT), its application in treatment deep-tissue metastatic tumours remains challenging. To extend applicability PDT, a novel chemiluminescent photosensitizer, Cy7-EOM, was developed by covalently coupling photosensitizer Cy7 with peroxycatechol derivative and encapsulating it within folate-modified disulfide-containing nano-micelles. Upon targeted delivery selective release, positive charged Cy7-EOM would target mitochondria efficiently generate singlet oxygen (1O2) via intramolecular chemiluminescence resonance energy transfer (CRET) endogenous H2O2, directly inducing mitochondrial damage cell apoptosis, realizing an efficient PDT for tumours. Remarkably, covalent linkage between donor acceptor greatly reduces distance, significantly enhancing CRET efficiency. Moreover, tumour-specific decomposition nano-micelles prevents aggregation-induced quenching mitigates diffusion barrier 1O2, while normal tissues integrality shields lethal effects 1O2. This method provides new strategy transforming adjuvant photosensitizers into direct therapeutic drugs, significant potential clinical

Language: Английский

Citations

0
GSH-responsive nanomedicine for disease smart imaging and therapy DOI

Zi Fu,

Di Zhou, Zeyang Liu

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 533, P. 216554 - 216554

Published: Feb. 27, 2025

Language: Английский

Citations

0
Development of time-resolved luminescence measurement instruments for biosensing and bioimaging – An overview DOI
Benjamin Sreenan, Vala Kafil,

Donovan Wells

et al.

Measurement, Journal Year: 2025, Volume and Issue: unknown, P. 117201 - 117201

Published: March 1, 2025

Language: Английский

Citations

0
Ultrabright difuranfluoreno-dithiophen polymers for enhanced afterglow imaging of atherosclerotic plaques DOI

Zhe Li,

Hui Cao,

Youjuan Wang

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(13)

Published: March 26, 2025

Cardiovascular diseases, including stroke driven by atherosclerosis, remain a leading global health concern. Current diagnostic imaging modalities such as magnetic resonance fail to characterize oxidative stress within atherosclerotic plaques. Here, we introduce difuranfluoreno-dithiophen–based polymers designed for afterglow imaging, offering ultrabright luminescence, ultralow-power excitation (0.087 milliwatts per square centimeter), and ultrashort acquisition times (0.01 seconds). Through molecular engineering strategy, have optimized enhanced reactive oxygen species (ROS) generation capability, ROS capturing fluorescence quantum yield, resulting in an increase intensity (~130-fold) compared commonly used 2-methoxy-5-(2′-ethylhexyloxy)-1,4-phenylenevinylene polymer (MEHPPV). Additionally, developed ratiometric nanoparticles doped with stress–responsive molecules, enabling of markers plaque. This approach provides tool cardiovascular diagnostics, which is conducive the auxiliary diagnosis risk stratification atherosclerosis.

Language: Английский

Citations

0
“All-in-One” Photochemical Afterglow Nanoplatform Based on Perovskite Quantum Dots DOI
Jiamiao Yin, Qianwen Zhou, Yanzhong Li

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Photochemical reaction-based afterglow materials offer a promising solution to the tissue autofluorescence issues associated with real-time excitation in traditional fluorescence probes. Conventional photochemical systems typically consist of three components: photosensitizer, an energy cache unit (ECU), and emitter. However, their physical separation leads inefficient transfer. We present strategy for constructing "all-in-one" nanoplatform (AGNP) based on perovskite quantum dots (PQDs) enhance transfer efficiency by minimizing separation. Modified 1-pyrenecarboxylic acid (PCA), CsPbBr3 PQDs can serve as emitter, ECU–phenylacetic (ECU–COOH) host simultaneously. The intensity AGNP shows remarkable 30-fold enhancement compared separated ECU system, attributed decreased distance. also exhibits great versatility, enabling tunable emission across visible region. is further adopted vivo imaging signal-to-noise ratio 41. This work provides idea demonstrates potential background-free bioimaging.

Language: Английский

Citations

0
A dark-state–dominated photochemical upconversion afterglow via triplet energy transfer relay DOI Creative Commons

Hang Yuan,

Kuangshi Sun,

Xianlong Su

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(17)

Published: April 25, 2025

Photochemical afterglow materials have drawn considerable attention due to their attractive luminescent properties and great application potential. Considering the classical photochemical always exhibit poor luminescence, it is urgent gain fundamental understanding of main limiting factors. Here, we identified existence a dark-state triplet in process, an overwhelming percentage ~98.5% was revealed for this non-emissive state. Guided by these observations, proposed activate unprecedented energy transfer relay simultaneously harness singlet energy. Consequently, upconverted material constructed with amazing luminescence performance albeit its moderate fluorescence emission property. The generality strategy evidenced adaptation similar emitters varied wavelengths. optimized enabled time-gated upconversion bioimaging under ultralow-power excitation. This study not only reveals pathways but also paves way rational design bright ultralong lifetime.

Language: Английский

Citations

0
Kinetic Equation Modeling-Guided Luminescence Modulation in Photochemical Afterglow DOI
Yuetian Pei, Yiwei Fan,

Kuangshi Sun

et al.

The Journal of Physical Chemistry Letters, Journal Year: 2025, Volume and Issue: unknown, P. 4884 - 4892

Published: May 9, 2025

Photochemical reaction-based afterglow has been widely applied in information storage, biodetection, and bioimaging. It is achieved through a cascade of photophysical processes chemical reactions. However, comprehensive kinetic study its complex remains limited. In this work, we conducted numerical simulations the entire process based on reaction equations, focusing key identifying rate-determining step. By varying rate constants steps, provided theoretical insights into effectively regulating intensity lifetime. Furthermore, designed synthesized several derivative molecules for experimental validation, achieving optimization both Through integration analysis with develops an in-depth comprehension establishes robust framework molecular design photochemical related systems.

Language: Английский

Citations

0
PEGylated BODIPY Photosensitizer for Type I Dominant Photodynamic Therapy and Afterglow Imaging DOI
Hui Wen, Qihang Wu,

Xiujuan Xiang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(45), P. 61739 - 61750

Published: Oct. 30, 2024

Type I photodynamic therapy (PDT) exhibits outstanding therapeutic effects in hypoxic environments tumors, but the design of type photosensitizers (PSs), especially those with simple structures dramatic properties, remains a challenge. Herein, we report strategy for developing PSs one molecule afterglow luminescence. As proof concept, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) PS (BIP) bearing water-soluble poly(ethylene glycol) (mPEG550) chains is synthesized, and BIP can self-assemble into nanoparticles (BIPNs). Interestingly, BIPNs exhibit an O2•--triggered luminescence, which scarce, BODIPY derivatives. demonstrate dominant PDT at ultralow dose under both normoxic environments, significantly inhibit tumor growth irradiation. This work highlights high-performance luminescence excellent effects, underscoring significant potential versatile clinical theranostics.

Language: Английский

Citations

3
Enabling robust near-infrared afterglow polymers through cascade energy transfer DOI
Wei‐Guang Wang, Shuman Zhang, J.S.T. Huang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 499, P. 155836 - 155836

Published: Sept. 20, 2024

Language: Английский

Citations

2