Cell
state
transitions
are
fundamental
in
biology,
determining
how
cells
respond
to
environmental
stimuli
and
adapt
diseases
treatments.
surface-based
sensing
of
geno/phenotypes
is
a
versatile
approach
for
distinguishing
different
cell
types
states.
Array-based
biosensors
can
provide
highly
sensitive
platform
based
on
the
differential
interactions
each
element
with
surface
components.
In
this
work,
modular
polymer-based
supramolecular
multichannel
sensor
array
(FNP
sensor)
was
fabricated
by
encapsulating
hydrophobic
dye
(pyrene)
into
monolayer
positively
charged
fluorescent
polymer
through
flash
nanoprecipitation
(FNP).
We
utilized
one-polymer
discriminate
among
commonly
found
tumors:
4T1
cancer
cells,
NIH/3T3
fibroblast
RAW
264.7
macrophage
cells.
The
also
successfully
characterized
varying
ratios
cancer-associated
fibroblasts
(CAFs)
tumor-associated
macrophages
(TAMs).
This
single
provides
effective
discrimination
high
reproducibility,
providing
high-throughput
tool
diagnostic
screening
states
associated
progression.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(32), P. 13207 - 13216
Published: July 30, 2024
Staphylococcus
aureus
(S.
aureus)
is
abundant
in
nature
and
frequently
leads
to
various
health
issues.
Bacteriophages
as
obligate
intracellular
parasites
of
bacteria
have
the
ability
specifically
identify
infect
S.
aureus,
causing
bacterial
lysis
release
endogenous
catalase
(CAT).
The
released
CAT
triggers
conversion
H2O2
into
O2
H2O,
resulting
a
notable
decrease
UV
absorption
at
570
nm
concurrent
surge
photocurrent.
On
basis
this,
photoelectrochemical/colorimetric
dual-mode
biosensor
for
detection
was
developed.
In
photoelectric
mode,
reactions
involving
enzymes
occur
directly
solution,
requiring
only
simple
drop-coating
TiO2@CdS
onto
indium
tin
oxide
(ITO)
electrode
surface.
There
no
need
immobilizing
additional
biomolecules,
thereby
significantly
minimizing
nonspecific
adsorption
improving
biosensor's
stability
reproducibility.
For
colorimetry,
we
utilized
cost-effective
operationally
approach
based
on
KI
starch.
Remarkably,
this
photoelectrochemical/colorimetry
exhibited
linear
range
102–109
CFU/mL
achieving
limits
7
10
CFU/mL,
respectively.
Herein,
phage
identification
ensures
specific
live
effectively
mitigating
potential
false
signals.
dual-signal
readout
mode
improves
accuracy
reliability.
conclusion,
present
method
offers
numerous
advantages,
including
simplicity,
time-efficiency,
cost-effectiveness,
high
specificity,
therefore
excellent
accuracy.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(36), P. 14490 - 14498
Published: Aug. 26, 2024
Bacterial
infections
have
emerged
as
a
significant
contributor
to
global
mortality
and
morbidity
rates.
Herein,
we
introduce
dual
fluorescence
"turn-on"
supramolecular
sensor
array
composed
of
three
assembled
complexes
(
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 9, 2025
Irritable
bowel
syndrome
(IBS)
is
a
globally
prevalent
functional
gastrointestinal
disorder
frequently
misdiagnosed
due
to
overlapping
symptoms
with
other
diseases.
Currently,
there
are
no
rapid
and
effective
diagnostic
or
therapeutic
approaches
for
IBS.
Despite
this,
low-FODMAP
diets
(LFDs)
have
become
major
dietary
intervention
strategy
symptom
relief.
However,
detecting
FODMAPs
usually
relies
on
chromatographic
techniques,
which
costly
time-consuming,
making
it
difficult
apply
in
real-time
detection.
In
this
study,
we
introduce
the
first
dual-functional
sensor
array
capable
of
rapidly
diagnosing
IBS
identifying
diets.
This
six-element
was
constructed
using
nitrophenylboronic
acid-modified
poly(ethylenimine)
coupled
coumarins
through
dynamic
borate
ester
bonds
across
range
pH
conditions.
Optimized
by
diverse
machine
learning
algorithms,
multilayer
perceptron
(MLP)
algorithm
proving
optimal,
enabled
simultaneous
identification
12
intestinal
bacteria
99.2%
accuracy
detection
mouse
fecal
specimens
varying
degrees
99.8%
within
seconds.
Furthermore,
allowed
various
FODMAP
levels
commercially
purchased,
brand-named,
differently
processed
soy
milk.
The
demonstrates
potential
use
both
clinical
diagnosis
guiding
patients.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 8, 2025
Nonspecific
interactions
are
ubiquitous
and
important
in
biology;
however,
they
rarely
valued
or
employed
the
field
of
clinical
disease
diagnosis.
Relying
on
nonspecific
cross-interactions,
sensor
arrays
have
shown
great
potential
distinguishing
mixtures
nuanced
compounds.
However,
developing
generic
strategies
for
constructing
effective
tailored
to
compositionally
complicated
highly
individualized
biospecimens
remains
challenging.
Here,
we
introduce
a
combinatorial
chemistry-screened
array
strategy,
leveraging
four-component
Ugi
reactions
achieve
rapid
synthesis
hundreds
structurally
diverse
sensing
elements.
Moreover,
can
thus
be
built
by
rapidly
screening
sensors
against
analytes.
Next,
demonstrate
practical
applicability
this
sperm
quality
assessment,
given
current
lack
well-established
detection
techniques.
A
library
192
elements
was
synthesized.
Following
screening,
pruned
14-element
successfully
constructed,
achieving
94.2%
accuracy
between
healthy
individuals
four
types
abnormal
samples
from
patients
within
1
min.
This
universal
strategy
avoids
complex
design
greatly
improves
efficiency
construction,
offering
new
way
designing
arrays.
ACS Sensors,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 13, 2025
Prompt
and
reliable
bacterial
identification
antibiotic
susceptibility
testing
are
vital
for
combating
infections
drug
resistance.
Herein,
we
designed
a
bispecific
metabolic
monitoring
platform
that
targets
enzyme-catalyzed
biochemical
reactions
testing.
Specifically,
two
kinds
of
coreshell-structured
persistent
luminescence
nanoparticles
with
surface-confined
red
green
luminescence,
respectively.
The
were
functionalized
energy
acceptors
can
be
specifically
cleaved
by
enzymes.
amplified
the
Förster
resonance
transfer
(FRET)
efficacy
from
to
surface
acceptors,
even
though
diameter
exceeded
critical
size
FRET,
which
improved
sensitivity
enzyme
monitoring.
Due
differentiated
expression
secretion
enzymes,
different
species
bacteria
produced
discrepant
after
incubation
nanoprobes.
Machine
learning
models
trained
characteristic
patterns
unknown
identification.
was
realized,
overall
accuracy
reached
100%.
Moreover,
machine
model
could
identify
active
inactive
states
treated
antibiotics,
provided
prompt
convenient
method
determine
whether
susceptible
antibiotics.
This
study
provides
robust
monitor
metabolism
offers
promising
strategy
infection
treatment,
communication
monitoring,
pathogenicity
investigation.
In
the
past
decades,
conjugated
polyelectrolytes
(CPEs)
have
become
prominent
in
sensing
applications
due
to
their
unique
properties,
including
strong
and
tunable
light
absorption,
high
sensitivity,
water
solubility,
biocompatibility.
Inspired
by
mammalian
olfactory
gustatory
systems,
CPE-based
sensor
arrays
made
significant
strides
discriminating
structurally
similar
analytes
complex
mixtures
for
various
applications.
This
review
consolidates
recent
advancements
arrays,
highlighting
rational
design,
controllable
fabrication,
effective
data
processing
methods.
It
covers
fundamentals
of
CPE
fluorescence
sensing,
emphasizing
design
strategies
array
units
techniques.
The
broad
applicability
is
demonstrated
across
diverse
domains,
environmental
monitoring
(e.g.,
detecting
metal
ions
explosives),
medical
diagnostics
disease
markers
analyzing
biological
samples),
food
safety
assessing
freshness,
quality,
source
products).
Further,
challenges
future
directions
field
are
discussed
inspire
further
research
development
this
area.
