Omnipolyphilins A and B: Chlorinated Cyclotetrapeptides and Naphtho-α-pyranones from the Plant Nematode-Derived Fungus Polyphilus sieberi

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(13), P. 6998 - 7009

Published: March 20, 2024

Chemical exploration for two isolates of the recently described ascomycete species Polyphilus sieberi, derived from eggs plant parasitic nematode Heterodera filipjevi, afforded identification many compounds that belong to various metabolite families: previously undescribed chlorinated cyclotetrapeptides, omnipolyphilins A (1) and B (2), one new pyranonaphthoquinone, ventiloquinone P (3), a 6,6′-binaphto-α-pyranone dimer, talaroderxine D (4) in addition nine known metabolites (5–13) were isolated this biocontrol candidate. All characterized by comprehensive 1D, 2D NMR, HR-ESI-MS analyses. The absolute configurations cyclotetrapeptides determined combination advanced Marfey's method, ROE correlation aided conformational analysis, TDDFT-ECD calculations, while ECD Mosher's experimental spectra used (3) (4). Among compounds, showed potent antimicrobial activities against Bacillus subtilis Staphylococcus aureus with MIC values 2.1 8.3 μg mL–1, respectively. Additionally, promising inhibitory effects on formation S. biofilms observed up concentration 0.25 mL–1. Moreover, ophiocordylongiiside (10) activity free-living Caenorhabditis elegans.

Language: Английский

---

Taxol is NOT produced sustainably by endophytic fungi ! – A case study for the damage that scientific papermills can cause for the scientific communities

Fungal Biology Reviews, Journal Year: 2024, Volume and Issue: 49, P. 100367 - 100367

Published: May 6, 2024

Over three decades ago, the plant-derived anticancer agent taxol (brand name: paclitaxel) was reported from a fungal endophyte colonizing producing plant. The hope that this finding could ever result in sustainable production process has thus far been disappointed. Modern evidence on evolution of secondary metabolites plants vs. fungi suggests hypothesis (that produce such complex plant metabolites) is invalid. Still, numerous inconclusive original studies -and particular, review papers by non-experts field-are continuously being published claim opposite. current commentary tries to deal with topic, taking findings –OMICS and state-of-the art mycology into account. This can hopefully help stop scientific papermills further spreading fake news were capable taxol.

Language: Английский

---

Transcriptomics-Driven Discovery of New Meroterpenoid Rhynchospenes Involved in the Virulence of the Barley Pathogen Rhynchosporium commune

ACS Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Rhynchosporium commune, the causal agent of barley scald disease, poses a major threat to global production. Despite its significant impact, molecular mechanisms underlying R. commune's infection process remain largely unexplored. To address this, we analyzed differential gene expression data commune WAI453 cultivated under both in planta and vitro conditions, aiming identify secondary metabolite biosynthetic clusters that are potentially involved pathogenicity commune. Our analysis revealed increased polyketide-terpene cluster (the rhy cluster), containing specific myeloblastosis (MYB)-type transcription factor rhyM, during growth. Overexpression rhyM an axenic culture activated cluster, resulting production series new meroterpenoid metabolites, which named rhynchospenes A-E. Their structures were elucidated through combination spectroscopic methods single crystal X-ray diffraction analysis. Infiltration into leaves resulted strong necrosis, with rhynchospene B demonstrating highest phytotoxicity causing necrosis at minimum concentration 50 ppm. Silencing confirmed role as virulence factors disease. The mutant showed significantly reduced compared wild-type strain decreased seedling assays on barley. characterization provided insights metabolites disease development. study also highlights potential use MYB-type overexpression uncovering cryptic SMs host adaptations.

Language: Английский

---

Fungi: Pioneers of chemical creativity – Techniques and strategies to uncover fungal chemistry

IMA Fungus, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Natural product discovery from fungi for drug development and description of novel chemistry has been a tremendous success. This success is expected to accelerate even further, owing the advent sophisticated technical advances that recently led an unparalleled biodiversity in fungal kingdom. review aims give overview on i) important secondary metabolite-derived drugs or leads, ii) discuss analytical strategic framework how natural lead identification transformed earlier days present, iii) knowledge biology facilitates new compounds, iv) point out endeavors understanding metabolite order systematically explore genomes by utilizing synthetic biology. An outlook given, underlining necessity collaborative cooperative scenario harness full potential metabolome.

Language: Английский

---

Modern Analytical Techniques for Quality Control and Chemical Identification of Phytochemicals

Published: March 13, 2025

Language: Английский

---

Reaping the Chemical Diversity of Morinagamyces vermicularis Using Feature-Based Molecular Networking

Journal of Natural Products, Journal Year: 2024, Volume and Issue: 87(9), P. 2335 - 2342

Published: Sept. 16, 2024

Moringadepsin (

Language: Английский

---

A review of the pharmaceutical applications of endophytic fungal secondary metabolites

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 17

Published: Nov. 8, 2024

A major challenge to human health is the emergence of drug-resistant pathogenic strains organisms. Studies have found ecologically friendly, cost-effective, and innocuous alternative sources bioactive compounds capable managing menace. This review x-rays endophytic fungal community pharmaceutical applications their secondary metabolites. Endophytic fungi house biologically active compounds, which makes them a good alternative. Also, intrinsic ability produce such an avalanche could be attributed mutualistic interaction with plant's host. Secondary metabolites harvested from been identified categorised: steroids, xanthones, terpenoids, isocoumarins, phenols, tetralones, benzopyranones, enniatrines. also highlights optimisation strategy, co-culture method, chemical epigenetic remodelling, molecular method as approaches adopted boost production compounds. The numerous were equally presented, include properties, well use in industries.

Language: Английский

---

Tailored culture strategies to promote antimicrobial secondary metabolite production in Diaporthe caliensis: a metabolomic approach

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Dec. 5, 2024

Language: Английский

---