A Bone‐Targeting Hydrogen Sulfide Delivery System for Treatment of Osteoporotic Fracture via Macrophage Reprogramming and Osteoblast‐Osteoclast Coupling DOI Open Access
Yi Qin, He Zhang, Xiaobin Guo

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract The demand for systemic treatment of osteoporotic fractures to reduce recurrence is increasing, but current anti‐osteoporosis medications exhibit unsatisfactory efficacy due adverse events and limited effects on fracture healing. Herein, a bone‐targeting zeolitic imidazolate framework‐8 (ZIF)‐based hydrogen sulfide (H 2 S) delivery system (ZIF‐H S‐SDSSD) designed simultaneously promote healing alleviate osteoporosis. With peptide SDSSD grafted the surface, ZIF‐H S‐SDSSD nanoparticles release H S in bone tissues without affecting serum level, thereby mitigating potential risks systematic delivery. Upon cellular uptake, acidic environment lysosomes drives from encapsulated zinc conjunction with degradation ZIF. synergistic released Zn 2+ macrophage metabolic reprogramming by suppressing succinate accumulation mitochondrial reactive oxygen species (mtROS) production, further regulate osteoblast‐osteoclast coupling. Overall, this strategy holds great promise clinical broadens application nanomedicine therapy orthopedic diseases.

Language: Английский

Dual Roles of Reducing Systems in Protein Persulfidation and Depersulfidation DOI Creative Commons
Zhichao Liu, Nicolas Rouhier, Jérémy Couturier

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 101 - 101

Published: Jan. 16, 2025

The oxidative modification of specific cysteine residues to persulfides is thought be the main way by which hydrogen sulfide (H2S) exerts its biological and signaling functions. Therefore, protein persulfidation represents an important thiol-switching mechanism as other reversible redox post-translational modifications. Considering their reductase activity but also connections with proteins that generate H2S related molecules, glutaredoxin (GRX) thioredoxin (TRX)-reducing systems have potential dual roles in both depersulfidation. In this review, we will first focus on recent advances describing physiological pathways leading before discussing TRX glutathione/GRX-reducing persulfidation/depersulfidation.

Language: Английский

Citations

1
CSE/H2S Signaling Pathways in Enhancing Muscle Function and Insulin Sensitivity During Exercise DOI Open Access
Miaomiao Xu, Xiaoguang Liu,

Danhong Hu

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1741 - 1741

Published: Feb. 18, 2025

Exercise plays a crucial role in maintaining metabolic health, enhancing muscle function, and improving insulin sensitivity, thereby preventing diseases such as type 2 diabetes. Emerging evidence highlights the significance of cystathionine γ-lyase (CSE)/hydrogen sulfide (H2S) signaling pathway pivotal regulator molecular physiological adaptations induced by exercise. This review comprehensively examines biosynthesis metabolism H2S, its distribution different tissues, mechanisms which CSE/H2S influences contraction, repair, protein synthesis. Additionally, it explores how modulates pathways, glucose uptake, lipid metabolism, sensitivity. The potential H2S donors exercise supplements is also discussed, highlighting their ability to improve performance health. Current research advancements, including application multi-omics approaches, are reviewed provide deeper understanding complex networks involved. Furthermore, challenges future directions addressed, emphasizing need for further mechanistic studies clinical applications. underscores therapeutic targeting optimize benefits

Language: Английский

Citations

0
Constructing a biomimetic TiOF2@PCN-222-Fe Z-scheme heterojunction using self-assembled L-cysteine for CO2 visible light photoreduction DOI

Ping Yi,

Chuanjiao Wang,

Chentao Hou

et al.

Materials Horizons, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Biomimetic synthesis via MOF-based catalysts to construct a Z-scheme heterojunction provides opportunities for enhancement of visible light photocatalytic CO 2 reduction efficiency.

Language: Английский

Citations

0
Mining proteomes for zinc finger persulfidation DOI Creative Commons

Haoju Li,

Andrew T. Stoltzfus,

Sarah L. J. Michel

et al.

RSC Chemical Biology, Journal Year: 2024, Volume and Issue: 5(6), P. 572 - 585

Published: Jan. 1, 2024

A meta-analysis of 22 persulfide-specific proteomics datasets reveals widespread persulfidation zinc finger proteins across various species, highlighting the role as an important post-translational modification.

Language: Английский

Citations

3
Bio-Pathological Functions of Posttranslational Modifications of Histological Biomarkers in Breast Cancer DOI Creative Commons

Anca-Narcisa Neagu,

Claudiu-Laurentiu Josan,

Taniya Jayaweera

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(17), P. 4156 - 4156

Published: Sept. 2, 2024

Proteins are the most common types of biomarkers used in breast cancer (BC) theranostics and management. By definition, a biomarker must be relevant, objective, stable, quantifiable biomolecule or other parameter, but proteins known to exhibit variate profound structural functional variation. Thus, proteome is highly dynamic permanently reshaped readapted, according changing microenvironments, maintain local cell tissue homeostasis. It that protein posttranslational modifications (PTMs) can affect all aspects function. In this review, we focused our analysis on different PTMs histological BC. analyzed PTMs, including phosphorylation, acetylation, methylation, ubiquitination, SUMOylation, neddylation, palmitoylation, myristoylation, glycosylation/sialylation/fucosylation transcription factors, proliferation marker Ki-67, plasma membrane proteins, histone modifications. Most these occur presence cellular stress. We emphasized interfere with maintenance, turnover lifespan, nuclear subcellular localization, structure function, stabilization inactivation, initiation silencing genomic non-genomic pathways, transcriptional activities signaling mitosis, proteostasis, cell–cell cell–extracellular matrix (ECM) interactions, trafficking, PPIs. Moreover, orchestrate hallmark pathways dysregulated BC, playing both pro- and/or antitumoral context-specific roles DNA damage, repair stability, inactivation/activation tumor-suppressor genes oncogenes, phenotypic plasticity, epigenetic regulation gene expression non-mutational reprogramming, proliferative signaling, endocytosis, death, TME, invasion metastasis, epithelial–mesenchymal/mesenchymal–epithelial transition (EMT/MET), resistance therapy reversal multidrug resistance. nucleus also at cytoplasmic level induce translocation opposite effects. Analysis allows for discovery validation new mainly early diagnosis, like extracellular vesicle glycosylation, which may considered as potential source circulating biomarkers.

Language: Английский

Citations

2
Synthesis, Characterization, and Reactivity of a Synthetic End-On Cobalt(II) Alkyl Persulfide Complex as a Model Platform for Thiolate Persulfidation DOI

Keyan Li,

Lev N. Zakharov, Michael D. Pluth

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(31), P. 21999 - 22007

Published: July 24, 2024

Persulfides (RSS

Language: Английский

Citations

1
Cysteine-rich zinc finger proteins and the nuclear factor kappa-B pathway DOI Creative Commons

Andrew T. Stoltzfus,

Sarah L. J. Michel

Frontiers in Chemical Biology, Journal Year: 2024, Volume and Issue: 3

Published: Dec. 20, 2024

Inflammation-related disorders, such as autoimmune diseases and cancer, impose a significant global health burden. Zinc finger proteins (ZFs) are ubiquitous metalloproteins which regulate inflammation many biological signaling pathways related to growth, development, immune function. Numerous ZFs involved in the nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) pathway, associating them with inflammation-related that feature chronically elevated pro-inflammatory cytokines. This review highlights predominance NFκB-related summarizes breadth functions these perform. The cysteine-specific post-translational modification (PTM) persulfidation is also discussed context cysteine-rich ZFs, including what known from few available reports on functional implications ZF persulfidation. Persulfidation, mediated by endogenously produced hydrogen sulfide (H 2 S), has recently established role inflammation. work will summarize connections between potential inform development therapies.

Language: Английский

Citations

0
Zinc and RNA Binding Is Linked to the Conformational Flexibility of ZRANB2: A CCCC-Type Zinc Finger Protein DOI
Matthew S. Hursey,

Abigail D. Reitz,

Kyle C. Kihn

et al.

Biochemistry, Journal Year: 2024, Volume and Issue: 64(1), P. 156 - 169

Published: Dec. 16, 2024

Ran-binding domain-containing protein 2 (ZRANB2) is a zinc finger (ZF) that plays key role in alternative splicing. ZRANB2 composed of two ZF domains contain four invariant cysteine residues per domain. binds RNA targets AGGUAA sequence motifs. Three constructs ZRANB2, ZRANB2-ZF1 (first domain), ZRANB2-ZF2 (second and ZRANB2-2D (both domains), were isolated the apo form shown to bind Zn(II) via UV-visible-monitored competitive titrations with Co(II) as spectroscopic probe. Zn binding each construct led adoption limited secondary structure domain, measured by circular dichroism (CD). Hydrogen-deuterium exchange coupled mass spectrometry (HDX-MS) two-domain construct, ZRANB2-2D, revealed both adopt more rigid upon binding. first domain resulted greater decrease conformational dynamics than second TRA2B pre-mRNA, physiological splicing target, was fluorescence anisotropy (FA), high-affinity found require coordination domains. HDX-MS well optimized sequences single double hexamer additional protection from H/D for presence RNA. Here, observed suggesting larger effect on dynamics. A model zinc-mediated proposed.

Language: Английский

Citations

0
A Bone‐Targeting Hydrogen Sulfide Delivery System for Treatment of Osteoporotic Fracture via Macrophage Reprogramming and Osteoblast‐Osteoclast Coupling DOI Open Access
Yi Qin, He Zhang, Xiaobin Guo

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract The demand for systemic treatment of osteoporotic fractures to reduce recurrence is increasing, but current anti‐osteoporosis medications exhibit unsatisfactory efficacy due adverse events and limited effects on fracture healing. Herein, a bone‐targeting zeolitic imidazolate framework‐8 (ZIF)‐based hydrogen sulfide (H 2 S) delivery system (ZIF‐H S‐SDSSD) designed simultaneously promote healing alleviate osteoporosis. With peptide SDSSD grafted the surface, ZIF‐H S‐SDSSD nanoparticles release H S in bone tissues without affecting serum level, thereby mitigating potential risks systematic delivery. Upon cellular uptake, acidic environment lysosomes drives from encapsulated zinc conjunction with degradation ZIF. synergistic released Zn 2+ macrophage metabolic reprogramming by suppressing succinate accumulation mitochondrial reactive oxygen species (mtROS) production, further regulate osteoblast‐osteoclast coupling. Overall, this strategy holds great promise clinical broadens application nanomedicine therapy orthopedic diseases.

Language: Английский

Citations

0