The Impact of Metagenomics on Biocatalysis

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(21)

Published: March 18, 2024

Abstract In the ever‐growing demand for sustainable ways to produce high‐value small molecules, biocatalysis has come forefront of greener routes these chemicals. As such, need constantly find and optimise suitable biocatalysts specific transformations never been greater. Metagenome mining shown rapidly expand toolkit promiscuous enzymes needed new transformations, without requiring protein engineering steps. If is needed, metagenomic candidate can often provide a better starting point than previously discovered enzyme on open database or from literature, instance. this review, we highlight where metagenomics made substantial impact area in recent years. We review discovery unexplored ‘hidden’ sequence space, leading characterisation with enhanced properties that originate natural selection pressures native environments.

Language: Английский

---

Metagenomic exploration of novel β-galactosidases for glycosylation engineering

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132546 - 132546

Published: April 1, 2025

Language: Английский

---

Accelerating enzyme discovery and engineering with high-throughput screening

Natural Product Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Recent progress in the DBTL cycle, including machine learning, facilitated enzyme mining for biocatalysis. Automation and standardization of library construction, coupled to high-throughput screening, further accelerates discovery process.

Language: Английский

---

Broadening The Substrate Scope of Aldolases Through Metagenomic Enzyme Discovery

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Bio‐processes based on enzymatic catalysis play a major role in the development of green, sustainable processes, and discovery new enzymes is key to this approach. In work, we analysed ten metagenomes retrieved 48 genes coding for deoxyribose‐5‐phosphate aldolases (DERAs, EC 4.1.2.4) using sequence‐based These sequences were recombinantly expressed Escherichia coli screened activity towards range aldol additions. Among these, one enzyme, DERA‐61, proved be particularly interesting catalysed addition furfural or benzaldehyde with acetone, butanone cyclobutanone unprecedented activity. The product these reactions, aldols, can find applications as building blocks synthesis biologically active compounds. Screening was carried out identify optimized reaction conditions targeting temperature, pH, salt concentrations. Lastly, kinetics stereochemistry products investigated, revealing that DERA‐61 other metagenomic DERAs have superior stereoselectivity when they are provided non‐natural substrates, compared well‐known DERAs.

Language: Английский

---

Tackling Global Biogeography and Drivers of Soil Microbial Dehalogenation Traits and Taxa: Insights from Metagenomic Profiling based on a Curated Dehalogenase Database

Soil Biology and Biochemistry, Journal Year: 2024, Volume and Issue: 198, P. 109553 - 109553

Published: Aug. 14, 2024

Language: Английский

---